Physical Sciences and Mathematics Commons^™

(Ultra-Rapid) Fluorescent Labelling Of Proteins, Cynthia Elaine Wilson

Honors Theses

The goal at the outset of this research project was to prepare fluorescent labeled bovine plasma albumin. A reprint of "Ultra-Rapid Fluorescent Labelling of Proteins" by Dr. Henry Rinderknecht, Director of California Corporation for Biochemical Research, Los Angeles, California, was secured to serve as reference material for the project.

The Thermodynamics Of Transition Metal Chelation In Dilute Aqueous Solution.|Ni.|Pthe Thermodynamics Of Interaction Of Serinate Ion With Divalent Co, Ni, Cu, Zn, And Cd Ions In Aqueous Solution.|Nii.|Pthe Calorimetric Standardization Of Dilute Ferrous Ion Solutions For Use In Determining 2,2'-Bipyridine, Joseph Willard Richards

Theses and Dissertations

Calorimetrically determined ΔH° values are given for the stepwise formation of chelate species between serinate ion and the divalent metal ions of Co, Ni, Cu, Zn, and Cd. These values of ΔH° are compared with those reported in the literature and were combined with values of ΔG° (log K) found in the literature to calculate values of ΔS°. These values of ΔG° and ΔS° are listed also. A new method of standardizing dilute ferrous ion solutions is reported. This procedure makes use of the thermometric titration technique to detect the equivalence point for the reaction of K_2Cr_2O_7 with ferrous ion. …

The Synthesis And Reactions Of Isatoic P-Linear Bis Anhydride, Willis G. Frick

Chemistry Honors Papers

This 41 page thesis examines methods of synthesizing isatoic anhydrides.

Knudsen- And Torsion- Effusion Measurements Of The Vapor Pressure Of Manganese(Ii) Fluoride, Walter Clayton Hitchingham

Masters Theses

No abstract provided.

A Study Of V(Iii) - V(Iv) And Ti(Iii) - Ti (Iv) Isotopic Exchange Reactions In Acid Solutions, Charles J. Orth

Chemistry and Chemical Biology ETDs

The subject of this dissertation is primarily the mechanisms and kinetics ot V(III) - V(IV) and Ti(III) - Ti(IV) electron exchange reactions in homogeneous solutions. The electron exchange process is a form of oxidation-reduction or electron transfer reaction where there is no net chemical change, i.e., the concentrations of the chemical species remain unchanged. In such cases there is a continuous exchange of electrons between a reduced and an oxidized form of the same element, such as between Fe(II) - Fe(III), V(III) - V(IV) or Tl(I) - Tl(III). The first two pairs involve one-electron changes while the thallium pair involves …

Electrochemical Measurements In Molten Fluorides, Howard W. Jenkins

Doctoral Dissertations

The nickel (II) / nickel couple, contained in a boron nitrade compartment, was shown to be a useful reference electrode in fluoride melts. From emf measurements no nickel (II) / nickel concentration cells, the nickel (II) / nickel couple was shown to obey the Nernst equation in molten LiF-NaF-KF (46.5-11.5-42.0 mole per cent) and LiF-BeF₂-ZrF₄ (65.6-29.4-5.0 mole per cent). It was found that in some cases the reference electrode had a significant junction potential across the boron nitride wall.

Kinetics of the charge transfer reaction Ni(II) + 2e = Ni were investigated by the voltage-step method. Apparent …

Chemical Ploughing, Geoffrey A. Pearce

Journal of the Department of Agriculture, Western Australia, Series 4

PROGRESS in the field of chemical weed control has been spectacular in recent years. One field that is attracting interest is the idea of replacing cultivation with chemical sprays.

Reactions Of Difunctional Esters With Benzyl 2-Amino-4,6-0- Benzylidene-2-Deoxy-D-Glucopyranosides, Fred Robert Seymour

University of the Pacific Theses and Dissertations

This research is primarily interested in investigating the reactions of benzyl 2-amino-4,6-0-benzylidene-2- deoxy-D-glucopyranoside (I) with various esters. The advantage offered by this sugar is that an alcohol and an amine group are in close proximity, allowing studies of intermolecular vs. intramolecular reactions and observation on anchimeric assistance in intramolecular reactions.

How Important Is Sulphur For Pastures?, R N. Glencross, W. J. Cox

Journal of the Department of Agriculture, Western Australia, Series 4

With the introduction of low sulphur compound and concentrated fertilisers, a new look is required at the sulphur status of West Australian soils, and the sulphur requirement of pasture.

In this article the functions of sulphur, deficiency symptoms and potential problem soils are described.

Current recommendations and plans for future research work are outlined.

The Control Of Weeds In Cereals, Geoffrey A. Pearce

Journal of the Department of Agriculture, Western Australia, Series 4

PRACTICALLY all broadleaved weeds found in cereals can now be killed with herbicides and with most, the farmer has a choice of chemicals.

Two more new products are now available and these have several advantages over other herbicides.

Kinetics Of The Decomposition Of The Cupric Ion Sulfhydryl Bond In Beef Plasma Albumin, Gary Rice

Honors Theses

The purpose of this work was to study the kinetics of the binding of cupric ion to beef plasma albumin, in particular the decomposition of the Cu++--BPA complex which absorbs light at 375 millimicrons.

The work of Klotz, Urquhart, Klotz, and Ayers has shown that the characteristic absorption band at 375 mu produced by the binding of cupric ion to beef plasma albumin is due to a copper- sulfhydryl bond. They have further demonstrated that the disappearance of this absorption band with time is accompanied by irreversible changes in the structure of the protein. The intensity of the initial absorption …

Ionium Age Determination, Leon Johnson

Honors Theses

Natural radioactive decay provides a means of determining the age of geological specimens. It is found that the number of atoms of a radioactive isotope disintegrating per unit time is linearly proportional to the existing number of atoms and independent of exterior parameters.

Modification Of The Devonshire Formula For The Thermal Accommodation Coefficient Of Helium On Tungsten, D. Vincent Roach, Robert E. Harris

Chemistry Faculty Research & Creative Works

The thermal accommodation coefficients (a.c.) of 3He and of 4He on clean tungsten were calculated using the Devonshire formula for temperatures between 70 and 600°K using "realistic" Morse potential parameters. The calculated a.c. values for 3He and 4He and the calculated ratios of the 4He to 3He values are compared with experimental data. The agreement is satisfactory if allowance is made for the error in the transition probability calculation used in the Devonshire formula.

Liquid Scintillators.The Decomposition Of 2, 5-Diphenyloxazole In Ultraviolet Light. A Thin Layer Chromatography Stud, John W. Hustler

Chemistry and Chemical Biology ETDs

A study has been initiated in order to understand more completely the manner in which a model organic scintil­lator decomposes in ultraviolet light. The compound, 2,5-diphenyloxazole (PPO), was dissolved in toluene in quartz vials and irradiated with ultraviolet light for various intervals of time. Both. vacuum-dried and untreated samples of PPO \·1ere subjected to UV excitation in sodium-dried and water-saturated solutions of toluene. The effect of passing oxygen and nitrogen through the solutions was also studied with the confirmation that dissolved oxygen and water accelerated the decomposition leading to the formation above the meniscus of the vial of a white …

Chemical Firebreaks, Geoffrey A. Pearce

Journal of the Department of Agriculture, Western Australia, Series 4

MANY public bodies are making regular use of herbicides to create firebreaks. In Western Australia more than $200,000 is being spent on chemicals each year to protect property from fire.

In many situations where the buildings and property are of great value, the cost of the chemical becomes insignificant.

Michael Reactions Part Iv Syntheses With Carboalkoxycarbenes, Frederick Ralph Carman

Masters Theses

"A preliminary investigation has been made involving syntheses with dicarboalkoxycarbenes for the purpose of developing new heterocyclic and alicyclic synthese employing functionalized carbenes. The ultimate goals were the development of a stereospecific synthesis of trans fused and angularly substituted ring systems suitable for conversion to natural products and the development of useful synthetic paths to the azabullvalene system. Although these ultimate goals were not realized, new and improved syntheses of alkyl substituted anisoles and diazomalonic esters, including the first reported synthesis of di-tert-butyl-diazomalonate were accomplished. The addition of dicarboalkoxycarbenes to di-, tri- and tetra- substituted ethylenes, tropilidene and 7-aza-tropilidenes by …

An Attempt To Determine Molybdenum By Atomic Absorption Spectrophotometry, Gary Rice

Honors Theses

An attempt was made to work out a method for routinely determining molybdenum by atomic absorption spectrophotometry. A stock standard was prepared containing 100 ppm molybdenum as MO4. Sensitivity of the spectrophotometer was found to be zero for aqueous solutions in the 1-10 ppm range. A pH-dependence study of extraction of the molybdate with DDC and MIBK gave best results at about pH 4.

Noble Gases: A Record Of The Early Solar System, E. C. Alexander

Doctoral Dissertations

"Analyses of the noble gases in samples of iron meteorites are presented which indicate the following: 1. The inclusions of iron meteorites contain noble gases with a wide range of isotopic compositions. 2. The noble gas concentration and isotopic anomalies arc often grossly inhomogeneously distributed within a given inclusion and vary from inclusion to inclusion in a meteorite. 3. Silicate inclusions appear to be the most promising samples for I-Xe and Pu-Xe dating. 4. Graphite and troilite in addition to the silicate inclusion contain significant radiogenic ¹²⁹Xe anomalies. 5. The total I-Xe (and in the case of Toluca silicates total …

The Preparation Of Coordination Compounds Of Rhodium (Iii) And Glutamic Acid And Substituted Glutamic Acids, Herman William Kalberer

University of the Pacific Theses and Dissertations

The father of coordination chemistry was Alfred Werner (1866- 1919). Werner's theory was largely responsible for the renewed interest in, and rapid growth of, inorganic chemistry around the turn of the century. He postulated that there were two types of valence, primary and secondary, which correspond, in modern terminology, to oxidation state and coordination number. The primary valences must be either negative ions, neutral molecules or, occasionally even, positive ions (11). He also postulated that the secondary valences are directed in space about the central ion, not only in the solid state, but also when the complex is in solution. …

Progress In Weed Control, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

In May, 1967, the "Journal of Agriculture" printed a special issue on weed control, with the emphasis on chemicals for weed control in cereal crops.

This month, just two years later, enough new information has accumulated to warrant publication of another "theme" issue, this time with important articles on weed control in pastures, chemical firebreaks and chemical ploughing, as well as weed control in linseed and cereal crops.

The Use Of Pickled Wheat In Poultry Feeding, P Smetana

Journal of the Department of Agriculture, Western Australia, Series 4

FEEDING fungicides to poultry can result in harmful effects, to the birds themselves, and to their human consumers.

To prevent such effects it is advisable that wheat pickled with fungicides should not be included in poultry rations.

Semiempirical Determination Of The Hydrogen Bond Energy For Water Clusters In The Vapor Phase. I. General Theory And Application To The Dimer, Richard W. Bolandee, James L. Kassner, Joseph T. Zung

Physics Faculty Research & Creative Works

A simple semiempirical method is given for determining the hydrogen bond energy for water clusters in the vapor phase. This method is based on a general statistical-mechanical theory of clustering. The partition function for a system of clusters is used to determine the equilibrium distribution of clusters. In conjunction with available thermodynamic and spectroscopic data, the cluster equilibrium constants can be used to calculate the cluster potential energy and the hydrogen bond energy. Results for the water dimer agree quite well with other reported values obtained either by quantum-mechanical calculations or approximate thermodynamic estimates. A correct temperature dependence of the …

Studies In Syntheses Of Non-Isoprenoid Sesquiterpenes, Raojibhai Javerbhai Patel

Masters Theses

The purpose of this study was to find the shortest route to prepare the 1,9-dimethyl decalone system I, a key intermediate in the synthesis of non-isoprenoid sesquiterpenes. The decalone system I was prepared in good yield by dehydrating the hydroxyketone II with 50% H₂SO₄. The dehydration of hydroxyketone II was also affected by heating in the presence of iodine crystals to yield 80% of the decalone system I.

The basic kinds of approaches under investigation were the Diels-Alder reaction and the Robinson annellation. The Diels-Alder reaction between the conjugated diene, 1-acetoxybutadiene (III) and 2,3-dimethylcyclohexenone (IV) under …

Complexes Of Phosphine Ligands With The Main Transition Elements, Richard R. Clikeman '69

Honors Projects

It has been long known that ammonia as well as many primary, secondary and tertiary amines, acts as a ligand forming complexes with the main transition elements.

The Vapor-Liquid And Liquid-Liquid Phase Equilibria For The Partially Miscible System Methanol-Cyclohexane, Richard A. Christman

Masters Theses

By use of techniques of previous workers for determining liquid-liquid equilibrium and through the use of a circulation still, the complete phase diagram for the partially miscible system, methanol-cyclohexane, was determined. Tile accuracy of earlier liquid-liquid phase data was not improved upon although it was closely approximated. The critical solution temperature was found to be 46.4 degrees C., which is within the range of temperatures reported in earlier measurements.

Activity coefficients of methanol and cyclohexane were calculated from the phase boundaries of the boiling point diagram determined in the present investigation. These activity coefficients were in agreement with the Gibbs-Duhem …

Formation Cross Sections For Rb86 From Rb87 And Sr87 With Intermediate Energy Protons, Rae Worley Sawyer

Dissertations, Theses, and Masters Projects

No abstract provided.

The Preparation And Determination Of Some Of The Properties Of The Dicarboxylic Amino Acid Chelates Of Platinum(Ii) And Palladium(Ii), Gordon Harold Williams

University of the Pacific Theses and Dissertations

It is surprising that no one has considered that coordination might have some effect on the strength of the acid group. It is the intent of this study to fill this gap in the knowledge of amino-acid chemistry.

At about the time this study began on the effect of chelation on the strength of the uncomplexed acid group, the next two higher homologs of these dicarboxylic amino acids became commercially available. It was, therefore, decided that the problem of this research should be expanded to include all four of the acidic amino acids.

The research problem was also defined to …

Palladium Complexes Of Imino Acids, Kurt Alfredo Freund

University of the Pacific Theses and Dissertations

The nature of this study involves the preparation of coordination compounds of palladium (II) and three imino acids, namely pipecolinic acid, pyrrolidine-20Carbozylic acid and azetidine carboxylic acid.

The Incorporation Of Oxygen-18 Into Oxindole Acid By Cells Of Hygrophorous Conicus, William Green Allen

Masters Theses

"To understand the mechanism of a biological oxidation in which oxygen is incorporated into a substrate, it is necessary to determine the origin of the incorporated oxygen. The objective of this investigation was to determine the origin of the oxygen atom incorporated into indoleacetic acid (IAA) by the IAA oxidase of Hygrophorous conicus through the use of oxygen-18. To determine the source of the oxygen atom incorporated into IAA it is necessary to incubate H. conicus cells with tryptamine or IAA in the presence of ¹⁸O₂ and H₂¹⁶O in one case and ¹⁶O₂ and H₂¹⁸O in another. An examination of …

Thermochemical Investigations Of The Methanol-Isopropanol System, Elmer Lee Taylor

Masters Theses

"Heats of solution and partial molar excess volumes at infinite dilution were determined for n-butanol, acetone, chloroform, and water in pure methanol, pure isopropanol, and several mixtures of the two at 25.0⁰C. The partial molar excess enthalpies of methanol and isopropanol were also determined and were combined to obtain integral heats of mixing. All heat data were obtained using a calorimeter of the heat-leak design, containing approximately 300 ml of solvent. Sample sizes ranged for 0.1 to 8 ml. Individual heat measurements were reproducible to 0.05 calories and reported values are considered to be accurate to 1% + 0.5 calorie …