Physical Sciences and Mathematics Commons^™

Use Of Flow Cytometer In Breeding Festuca X Lolium Hybrids, M C. Kerlan, M Ghesquiere

IGC Proceedings (1993-2023)

Ploidy in various generic Lolium - Festuca progenies were assessed by flow cytometry and compared to conventional chromosome counting. In non-segregating triploid and tetraploid progenies, the cytometer estimated so accurately the level of plant ploidy that chromosomes counting would be no use. In segregant progenies, all the tetraploid plants according to the cytometer had effectively 28 chromosomes. Among the triploid and pentaploid plants detected by the cytometer, 40% of the plants were tetraploid. They represented only 29% of the progeny which must be counted. The flow cytometry can be used in routine interspecific breeding programmes. Conventional chromosome counting should be …

Persistency In Lolium X Festuca Hybrid Derivatives And Its Relationships With Flowering Traits, M Ghesquiere, C Mousset

IGC Proceedings (1993-2023)

Using amphiploid and backcrossed derivatives of Italian ryegrass x tall fescue hybrids, the paper emphasizes the interest of introgression vs amphiploidization in breeding for specific traits such as persistency or seed production from tall fescue or ryegrass resp. Persistency in amphiploid and Lolium-introgressed progenies were assessed in nursery together with variation of flowering traits. Persistency was found lower, on average, in introgressed progenies than in hybrid progenies but with enlarged variability within progeny suggesting possible advantageous rearrangements of fescue chromosomes. Although significantly associated, persistency in both populations was only very little affected by the variations in flowering traits such …

Field Evaluation Of Crown Rust In Annual Ryegrass Populations, G M. Prine

IGC Proceedings (1993-2023)

Crown rust (Puccinia coronata [Pers.] Cda.) is the most prevalent disease found on annual ryegrass (Lolium multiflorum L.) of the southeastern USA. Determination of crown rust resistance is difficult because of the erratic nature of infections at most locations. As crown rust is present at Gainesville, FL each year, a rust nursery was designed to evaluate rust resistance and susceptibility of annual ryegrass. Evaluation consists of periodic rust ratings of the seed crop and regrowth of part of each plot after cutting. Nursery results indicate the buildup of rust, including at the date of 90% heading (start of …

Selection Of Interspecific Hybrids Of Brachiaria-A Tropical Forage Grass, C B. Do Valle

IGC Proceedings (1993-2023)

The aim of the breeding program at the National Beef Cattle Research Center (CNPGC) of the Brazilian Corporation for Agricultural Research (EMBRAPA), is to produce vigorous apomictic hybrids with wide edaphic adaptation, good production and nutritive value combined with spittlebug (Homoptera:Cercopidea) resistance. Fiftysix superior hybrids were selected based on visual estimates of vigor, leafiness, tolerance to drought and natural spittlebug infestation in the field. These were characterized for mode of reproduction, spittlebug reaction under controlled conditions and for morphological diversity using nine selected traits. Multivariate analysis classified the population into seven groups where the hybrids were clearly intermediate in plant …

Mitochondrial Genome Polymorphism In Lolium Perenne, Y Shimamoto, Y Tominaga, M Sato, T Mikami

IGC Proceedings (1993-2023)

The restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA (mtDNA) of perennial ryegrass (Lolium perenne L.) were investigated to elucidate the genetic relatedness among the 128 cultivars including diploid and tetraploid. Many patterns of RFLPs were observed and allowed assigning of the cultivars into the main eight haplotypes of mitochondrial genome relatedness. The American cultivars were classified into haplotype I and VIII which were remote at the mitochondrial genome from each other, the European ones were distributed to all haplotypes and the tetraploid ones were mostly assigned into the haplotype V. The assessment of mtDNA RFLPs may be a …

Inbreeding Of Some Populations In The Genus Agrostis L., Zorica Tomic, Gordana Surlan Momirovic, Dusica Delic, S Ostojic

IGC Proceedings (1993-2023)

This work presents some properties on produced inbred lines of the 5 best populations of 4 species of Agrostis for breeding purposes. The cultivars of forage grasses should meet a given aim for selection, which is the increasing dry matter and quality. The populations used in this research were the best ones for morphological and productive properties from a previous study. By the process of selfing there have been produced S1 offsprings which appeared to be quite superior in relation to open pollinated offspring from the mother plants. The obtained results were processed by multiple range regression analyses. According to …

The Effect Of Abscisic Acid On The Freezing Tolerance In Lolium Temulentum L., K Tase, H Fujii

IGC Proceedings (1993-2023)

This study was conducted to clarify the effect of abscisic acid (ABA) on the development of freezing tolerance in Lolium temulentum L. We compared the changes of freezing tolerance and endogenous ABA content by the ABA treatment with those by the hardening treatment. Exogenous ABA applications (7.5 x 10-5 M) increased freezing tolerance, but did not completely substitute for the hardening process. This treatment resulted in great increase in endogenous ABA content, as compared with the control or the hardening treatment. Since the relationship between endogenous ABA content and freezing tolerance was not parallel, it is considered that freezing tolerance …

Introduction Of Vegetative And Reproductive Characters Into Trifolium Repens By Interspecific Hybridisation, A H. Marshall, T P.T Michaelson-Yeates, M Meredith, D H. Hides

IGC Proceedings (1993-2023)

Interspecific hybridization between white clover (Trifolium repens L.) and ball clover (T.nigrescens) and Caucasian clover (T.ambiguum) is a means of improving the reproductive growth and persistency of T.repens. F1 hybrids between T.repens and T.nigrescens were produced without ovule culture and confirmed as true hybrids by chromosome counts, isoenzyme analysis and leaf markers. Their growth habit was generally intermediate between the two parents. After backcrossing to T.repens the BC1 and BC2 hybrids had more of the characteristics associated with T.repens though they retained some of the greater reproductive growth of …

Colchicine-Doubling Of Germinating Seedlings Of Interspecific Wildrye Hybrids, T A. Jones, D C. Nielson, H Jaussi

IGC Proceedings (1993-2023)

Colchicine has usually been applied to sterile clones of interspecific grass hybrids to restore fertility. However, when hybrids are partially fertile, colchicine can be applied to germinating seedlings. Four populations of Basin wildrye (Leymus cinereus) X beardless wildrye (L. triticoides) were treated with a 0.2% colchicine solution to double chromosome number from 4x=28 to 8x=56. Doubling percentage was 28, 33, 42, and 44% of all root-tips examined for the four populations. When plants without doubled sectors were discarded, doubling percentage increased to 56, 68, 78, and 78%. Plants with doubled sectors have been placed in crossing blocks …

03-04-2024 Orsp Newsletter, Liz Williamson

ORSP Newsletter

ORSP On-demand Training for PIs, Liz Williamson spotlight, Grant for Studying Clean Energy Production Awarded, NIH Simplified Peer Review Framework.

Western Science And Eastern Zen To Seek The Origin Of Truth: Philosophical Background Of Scale Modeling, Kozo Saito

Progress in Scale Modeling, an International Journal

This article was written to introduce philosophical background of scale modeling, where Zen philosophy was applied to overcome the limitation of logical thinking and hypotheses-driven deductive science. Three specific reasons are as follows. The first is related to the law approach in scale modeling; it uses the kufu principle, originated in Zen Buddhism, together with the other three scientific methods: experimental, theoretical, and computational. The second reason is because scale modeling seeks relativistic understanding by attempting to realize similarity; the concept is closer to Eastern philosophy rather than absolute understanding cultivated by deductive science. The third is in the educational …

How Can Generative Ai (Genai) Enhance Or Hinder Qualitative Studies? A Critical Appraisal From South Asia, Nepal, Niroj Dahal

The Qualitative Report

Qualitative researchers can benefit from using generative artificial intelligence (GenAI), such as different versions of ChatGPT—GPT-3.5 or GPT-4, Google Bard—now renamed as a Gemini, and Bing Chat—now renamed as a Copilot, in their studies. The scientific community has used artificial intelligence (AI) tools in various ways. However, using GenAI has generated concerns regarding potential research unreliability, bias, and unethical outcomes in GenAI-generated research results. Considering these concerns, the purpose of this commentary is to review the current use of GenAI in qualitative research, including its strengths, limitations, and ethical dilemmas from the perspective of critical appraisal from South Asia, Nepal. …

Development Of An Integrated Workflow For Nucleosome Modeling And Simulations, Ran Sun

Doctoral Dissertations

Nucleosomes are the building blocks of eukaryotic genomes and thus fundamental to to all genetic processes. Any protein or drug that binds DNA must either cooperate or compete with nucleosomes. Given that a nucleosome contains 147 base pairs of DNA, there are approximately 4^147 or 10^88 possible sequences for a single nucleosome. Exhaustive studies are not possible. However, genome wide association studies can identify individual nucleosomes of interest to a specific mechanism, and today's supercomputers enable comparative simulation studies of 10s to 100s of nucleosomes. The goal of this thesis is to develop and present and end-to-end workflow that serves …

Plants Reduced Nitrous Oxide Emissions From A Northern Great Plains Saline/Sodic Soil, Sharon A. Clay, Thandiwe Nleya, David E. Clay, Deepak Joshi, Dwarika Bhattarai, Shin-Yi Marzano, Bhanu Prakash Petla

Agronomy, Horticulture and Plant Science Faculty Publications

The slowly establishing salt-tolerant perennial grasses reduced nitrous oxide (N2 O- N) emissions from saline/sodic soil compared to barren areas. Other salt-tolerant species may accelerate vegetative establishment and reduce N 2 O-N emissions. In a greenhouse study, barley (Hordeum vulgare L.), Florida broadleaf mustard (Brassica juncea L.), and Kernza intermediate wheatgrass [Thinopyrum intermedium (Host) Barkworth & D. R. Dewey] were grown for 63 days to compare shoot biomass and chemical composition, N 2 O-N emissions, and the soil microbiome between saline/sodic and productive (non-salt impacted) soils. Emissions were measured six times daily from 1 to 22 and 42 to 63 …

Comstock Point, Lubec, Maine - A Natural And Photographic History, Willaim H. Schlesinger, Lisa M. Dellwo

Documents from Environmental Organizations

A natural and photographic history of our lands compiled by Lisa Dellwo and Bill Schlesinger, with the help of many friends and neighbors.

Procedures For Rangeland Condition Assessment In The Kimberley 2022, Department Of Primary Industries And Regional Development

Natural resources published reports

This report defines the procedures used in 2022 by the Department of Primary Industries and Regional Development (DPIRD) to complete rangeland condition assessment (RCA) in the Kimberley region which has grass-based pastures. For the rest of the pastoral estate, DPIRD uses the Procedures for RCA in the Pilbara and southern rangelands which have predominantly shrub pastures.

RCA is the process DPIRD uses to make systematic, lease-level assessment of rangeland. Rangeland condition considers both the condition of pastures (as defined in the Pasture condition guide for the Kimberley, Ryan et al. 2013) and soils as determined by erosion, using the …

Field Research Report: Results From The Enreec Vri Field For The 2021, 2022, And 2023 Crop Seasons, Derek M. Heeren, Ali T. Mohammed, Eric Wilkening, Christopher M. U. Neale, Alan L. Boldt, Ankit Chandra, Precious Nneka Amori, Ivo Z. Goncalves, Yeyin Shi, Guillermo R. Balboa

Department of Biological Systems Engineering: Conference Presentations and White Papers

Long-term irrigation management research has been conducted from 2014 to 2023 for corn and soybean at the Eastern Nebraska Research, Extension, and Education Center (ENREEC) Variable Rate Irrigation (VRI) Field located in subhumid east-central Nebraska (in the Lower Platte North Natural Resources District). The objective of this report was to present the overall results from the VRI Field for 2021 to 2023. Across the three growing seasons, there were the following irrigation treatments: Best Management Practice (BMP), 50% BMP, 125% BMP, rainfed, Spatial ET Modeling Interface (SETMI), SDD1, SDD2, machine-learning-based Cyber-Physical System (CPS), a student team recommended rate, and industry …

Soil Nitrate Prediction Using Ftir-Atr, Vis-Nir, And Raman Spectroscopy, Sabiha Ferdous

Department of Biological Systems Engineering: Dissertations and Theses

Fourier transform infrared spectroscopy (FTIR) combined with Attenuated total reflectance (ATR), Visible-Near Infrared spectroscopy (Vis-NIR), and Raman spectroscopy (RS) are non-destructive techniques for rapid determination of nitrogen compounds in soil. Leveraging FTIR-ATR and Vis-NIR spectra using partial least squares regression (PLSR) modeling, the study aims to predict soil nitrate content and explored the feasibility of Raman spectroscopy to detect nitrate (NO₃^-), nitrite (NO₂^-), and ammonium (NH₄⁺) in soil. Soil samples were collected from four different fields, dried, sieved (2mm), and then used for collecting spectra (FTIR-ATR and Vis-NIR). Laboratory analysis was …

Pollinator Communities And Their Ecosystem Services At Conservation Grasslands And Adjacent Croplands, Araceli Gomez Villegas

Department of Entomology: Dissertations, Theses, and Student Research

Pollinators are intrinsically linked to the success of unmanaged and managed ecosystems by providing pollination services that aid in the reproduction of wildflowers and many crops. Land use change, habitat loss, fragmentation, and related landscape-level phenomena (for example, increased pesticide exposure) threaten pollinators and have been associated with population declines. In the Midwestern region of the United States, land conversion of native prairies and grasslands to row-crop agriculture has been one of the largest contributors to pollinator habitat loss. Conservation programs, such as the Conservation Reserve Program, have worked towards removing environmentally sensitive lands from agriculture production and enrolling them …

Wayne E. Sabbe Arkansas Soil Fertility Studies 2023, Nathan A. Slaton

Arkansas Agricultural Experiment Station Research Series

Rapid technological changes in crop management and production require that the research efforts be presented in an expeditious manner. The contributions of soil fertility and fertilizers are major production factors in all Arkansas crops. The studies described within will allow producers to compare their practices with the university’s research efforts. Additionally, soil-test data and fertilizer sales are presented to allow comparisons among years, crops, and other areas within Arkansas.

Method For Calibrating An Arduino-Based Soil Moisture Sensor Using Van Genuchten’S Equation, Victorino A. Bato, John Osbert Alindogan

The Philippine Agricultural Scientist

Instructions on building a do-it-yourself soil moisture sensor using Arduino microcontrollers abound on the Internet. These do-it-yourself soil moisture sensors are favorite projects by both plant and microcontroller enthusiasts because the parts are affordable and the end product is functional. In the absence of proper calibration, these soil moisture sensors cannot generate usable information on the status of plant-available-water in the soil. This research demonstrates the calibration of an Arduino-based soil moisture sensor using the soil moisture characteristic curve of a clay loam soil. Van Genuchten’s model was used to generate an equation and various parameters specific to the soil. …

Supply Is Not Limulus: Research Review Of Horseshoe Crab Conservation In The Face Of Intense Pharmaceutical Demand, Zoya Galeev

University Honors Theses

Horseshoe crabs are being used by the pharmaceutical industry to conduct endotoxin tests using LAL derived from the organism’s blood to ensure safe medical practice. Their annual collection and bleeding, while not always leading to mortality, affects horseshoe crab behavior and health. This research seeks to understand how the American horseshoe crab, L. polyphemus, is being used by pharmaceutical agencies and the implications that their harvesting has on the industry and the conservation of the species. Studies were collected from the past decade across two databases, Web of Science (WOS) and PubMed, to assess present conservation techniques to reduce …

Automated Tree Mortality Detection Using Ubiquitously Available Public Data, Michael T. Huggins

Master's Theses

Understanding the dynamic interplay between fire severity, topography, and tree mortality, is crucial for predicting future forest dynamics and enhancing resilience against climate change-induced wildfire regimes. This thesis develops a multi-sensor approach for automated estimation of tree mortality, then applies it to examine trends in tree mortality over a six-year period across a fire affected study site in the Trinity River basin in Northern California. The Random Forest model uses publicly available USGS 3D Elevation Program Lidar (3DEP) and NAIP imagery as inputs and is likely to be easily adaptable to other landscapes. The model had a Receiver Operating Characteristic …

A 2,000-Year Record Of Eelgrass (Zostera Marina L.) Colonization Shows Substantial Gains In Blue Carbon Storage And Nutrient Retention, Martin Dahl, Martin Gullström, Irene Bernabeu, Oscar Serrano, Carmen Leiva-Dueñas, Hans W. Linderholm, Maria E. Asplund, Mats Björk, Tinghai Ou, J. Robin Svensson, Elinor Andrén, Thomas Andrén, Sanne Bergman, Sara Braun, Anneli Eklöf, Zilvinas Ežerinskis, Andrius Garbaras, Petter Hällberg, Elin Löfgren, Malin E. Kylander, Pere Masqué, Justina Šapolaitė, Rienk Smittenberg, Miguel A. Mateo

Research outputs 2022 to 2026

Assessing historical environmental conditions linked to habitat colonization is important for understanding long-term resilience and improving conservation and restoration efforts. Such information is lacking for the seagrass Zostera marina, an important foundation species across cold-temperate coastal areas of the Northern Hemisphere. Here, we reconstructed environmental conditions during the last 14,000 years from sediment cores in two eelgrass (Z. marina) meadows along the Swedish west coast, with the main aims to identify the time frame of seagrass colonization and describe subsequent biogeochemical changes following establishment. Based on vegetation proxies (lipid biomarkers), eelgrass colonization occurred about 2,000 years ago after geomorphological changes …

Environmental Dna Metabarcoding Of Pan Trap Water To Monitor Arthropod-Plant Interactions, Joshua H. Kestel, David L. Field, Philip W. Bateman, Nicole E. White, Karen L. Bell, Paul Nevill

Research outputs 2022 to 2026

Globally, the diversity of arthropods and the plants upon which they rely are under increasing pressure due to a combination of biotic and abiotic anthropogenic stressors. Unfortunately, conventional survey methods used to monitor ecosystems are often challenging to conduct on large scales. Pan traps are a commonly used pollinator survey method and environmental DNA (eDNA) metabarcoding of pan trap water may offer a high-throughput alternative to aid in the detection of both arthropods and the plant resources they rely on. Here, we examined if eDNA metabarcoding can be used to identify arthropods and plant species from pan trap water, and …

Antioxidant Properties Of Kombucha Beverage Infused With Ganoderma Lucidum And Green Tea From Camellia Sinensis (L.) Kuntze With Several Fermentation Times, Rizki Rabeca Elfirta, Pamungkas Rizki Ferdian, Iwan Saskiawan, Tri Hadi Handayani, Kayla Faza Gustafri Mandalika, Rini Riffiani, Kasirah Kasirah, Ukhradiya Magharaniq Safira Purwanto

Karbala International Journal of Modern Science

Kombucha is a functional beverage produced through the fermentation of infused tea or Camelia sinensis (L.) Kuntze (CS) by a symbiotic culture of bacteria and yeast. Apparently, the substrate of kombucha can be substituted to enhance its functional properties. Ganoderma lucidum (GL) is a potential substrate reported to have health benefits. This study aims to evaluate antioxidant properties by comparing different formulations and fermentation times. The formulations prepared in the present study varied in the compositions of GL and CS substrates, namely F1 (100% GL), F2 (75% GL: 25% CS), F3 (50% GL: 50% CS), F4 (25% GL: 75% CS), …

Germplasm Enhancement Of Annual Forage Legumes Suitable For Use In Cold Highland Environments, H K. Firincioglu, N Karagullu, S Unal, A M. Abd-El Moneim

IGC Proceedings (1993-2023)

The Central Highlands (CH) of Turkey are characterized by cold winters and dry summers. Under these extreme air temperatures, forage crops are still underexploited as a part of crop rotations. Half of the total vetch hectarage in Turkey is in the CH, and farmers still use the local cultivars that have good adaptability but low yield potential. Therefore, with the aim of identifying and improving the annual forage legume species for the cold and dry environment of the CH, research work was carried out during the 1993/94 and 1994/ 95 crop seasons. Initial results showed that Hungarian vetch (Vicia …

Use Of The Grazing Animal In Forage Breeding, J H. Bouton, C S. Hoveland, R N. Gates

IGC Proceedings (1993-2023)

The objective of these studies was to determine, in the context of a forage breeding program, the effect of cattle grazing on survival of alfalfa (Medicago sativa L.), red clover (Trifolium pratense L.), and tall fescue (Festuca arundinacea Schreb.). Different cultivars of each species were compared in grazed and ungrazed areas. Results consistently demonstrated that grazing reduced plant stands when compared to ungrazed conditions for each species tested. In alfalfa, however, cultivars developed for grazing tolerance showed much better grazing survival. Infection with the fungal endophyte, Acremonium coenophialum Morgan-Jones & Gams, likewise substantially increased grazing survival in …

Study Of Genotype X Environment Interaction In Alfalfa Forage Yield, S N. Acharya, G C. Kozub, H Najda, A Aasen

IGC Proceedings (1993-2023)

The response of alfalfa (Medicago sativa L.) forage yield to eight Alberta test sites was studied for the 1990 and 1991 production years. Cluster analysis was used to group locations and cultivars. Analyses of variances indicated genotype x environment (location) interaction for the first cut yield, the total yield and the difference between first and second cut yields. The Brooks, Bow Island (irrigation), Bow Island (dryland) and Provost locations always clustered together indicating that three of these four test sites may be eliminated without sacrificing reliability.

Selection For Self-Fertility And Self-Fertility In Alfalfa As A Tool For Breeding Strategy Assessment, D Rosellini, F Veronesi, F Lorenzetti

IGC Proceedings (1993-2023)

Cultivar development in alfalfa relies on heterosis, that has been mainly exploited by intercrossing selected clones from diverse gemplasm sources; an alternative way was proposed, based on developing double cross cultivars obtained by crossing clones selected for self-sterility, propagated by somatic seed. A different breeding strategy was recently emphasized based on population improvement through inbreeding and recurrent selection; in this case, self-sterility would be selected against. If self-sterility is determined by genetic load, the second strategy should be preferred, and selecting for self-fertility may bring about positive correlated responses for forage yield. Divergent selection for self-fertility was applied in a …