Physical Sciences and Mathematics Commons^™

Recent Developments In Alternatives To Animal Testing, Katy Taylor

Application of Alternative Methods Collection

Methods that replace techniques that use live animals, or methods of testing substances without live animal use, are known as alternatives, replacements or non-animal methods. Some prefer the term advanced technologies given the fact that they often rely on more sophisticated technology and are more human- relevant than the animal test they replace (see Langley et al., 2015). There have been efforts to replace animal tests since the 1960s. Significant progress initially came in replacing animals used to diagnose human disease; to produce biological drugs (such as vaccines); and to safety test batches of these drugs as they were produced. …

The Changing Paradigm In Preclinical Toxicology: In Vitro And In Silico Methods In Liver Toxicity Evaluations, Fozia Noor

Application of Alternative Methods Collection

In vitro methods, based on human primary cells, cell lines, and genetically modified reporter cell lines, have greatly expanded the scope of in vitro toxicology. Other significant progress in the area of human-induced pluripotent stem cells (hiPSCs) (Asgari et al., 2010; Schwartz et al., 2014; Shinde et al., 2016; Shtrichman, Germanguz and Itskovitz-Eldor, 2013) is allowing the application of patient and disease-specific hiPSCs (Ghodsizadeh et al., 2010; McCracken et al., 2014; Siller et al., 2013). Moreover, the tools of precise genome editing with engineered nucleases, such as the zinc finger nucleases (zfns), the transcription activator-like effecter nucleases (talens) and, more …

How To Evaluate The Science Of Non-Human Animal Use In Biomedical Research And Testing: A Proposed Format For Debate, Ray Greek, Lisa A. Kramer

Validation of Alternative Methods Collection

Over time, the interpretation of science has occasionally been corrupted by vested interest groups, be they financially motivated or ego driven. Scientific consensus and widespread public beliefs usually catch up with the evidence, but this can take a very long time and often costs lives. The use of non-human animals in biomedical research and testing is a scientific endeavor and, as such, can and should be evaluated in light of the best science currently available. But facts that have been accepted in all areas of science are routinely ignored or called into question by well-funded, vested interest groups, compromising the …

How Can The Final Goal Of Completely Replacing Animal Procedures Successfully Be Achieved?, Christiane Baumgartl-Simons, Christiane Hohense

Validation of Alternative Methods Collection

Article 23 of European Union (EU) Directive 86/609/EEC required that Member States promote the development and validation of alternative technologies and stated that the European Commission (EC) “shall report before the end of 1987 on the possibility of modifying tests and guidelines” (European Parliament, 1986, Article 23). This Directive was replaced by Directive 2010/63/EU on the protection of animals used for scientific purposes, which now requires that Member States develop and validate alternative approaches much more precisely and specifies that the ultimate objective is the “full replacement of procedures on live animals for scientific and educational purposes, as soon as …

The Potential Of Organ On Chip Technology For Replacing Animal Testing, Malcolm Wilkinson

Human Clinical Trials Collection

The term organ on a chip is used to describe the latest stage of development of in vitro cell culture technology. Figure 26.1 shows its steady development since the 1960s. Each step forward has improved our ability to model human-clinical response to new drugs or therapies and has enabled safety risk assessment of existing cosmetics, personal care products, or other chemicals in the environment. Scientific evidence that the predictive power of in vitro tests is superior to the use of animals will trigger a major shift in the way that medical research, in many areas, is carried out. In this …

Effectiveness Of Plant Species For Removing Atmospheric Ammonia, Marife B. Anunciado, Sheryll B. Jerez, Hans Williams, Joey Bray, Dean W. Coble, Rena Saito

Faculty Publications

Six plant species of Yaupon, Eastern red cedar, American holly, Arizona cypress, Arborvitae and Roughleaf dogwood were utilized to determine their effectiveness in the removal of atmospheric ammonia. All species were exposed to three ammonia levels (1, 5 and 10 ppm) in an environmental chamber. Foliar ammonia content was quantified using an enzymatic technique. The effects of exposure to ammonia on the physiological responses (e.g. photosynthetic activity, stomatal conductance, and transpiration rate) of plants in ambient condition were also determined using an open design photosynthetic gas exchange system. Foliar ammonia content was significantly different among the six plant species (p<0.0001) with Eastern red cedar exhibiting the highest content. The physiological responses differed significantly depending on the plant species and the ammonia treatment level. The photosynthetic response of plants to the presence of ammonia was mixed. At low exposure level, all species except Arborvitae had decreased photosynthetic activity, reducing by as much as 44.5% for Yaupon. At the highest concentration, however, Yaupon’s photosynthetic activity improved by about 10%. Exposure to ammonia caused increased stomatal conductance and transpiration rate on American holly and Arizona cypress, making them more susceptible to water loss.

Case–Control Research Study Of Auto-Brewery Syndrome, Barbara Jean Cordell, Anup Kanodia, Gregory K. Miller

Faculty Publications

Background: Auto-brewery syndrome (ABS), also known as Gut Fermentation Syndrome and Endogenous Ethanol Fermentation, is afflicting people worldwide, but little is known about ABS patients’ demographics, health history, lifestyle factors, and diet.

Method: We conducted a broad-based case–control survey study on 52 patients known to have a diagnosis of ABS and their household members. The research compares the symptomatic group (N ¼ 28) to the asymptomatic group (N ¼ 18) regarding lifestyle and health, diet, and medical history.

Results: With a response rate of 88% and using rank-sum tests, the data demonstrate that patients with ABS have …

High Frequency Percussive Ventilation For Tumor Motion Immobilization, Ina Marina Sala

Wayne State University Dissertations

This work investigates the use of High Frequency Percussive Ventilation as a technique for respiratory motion mitigation in radiotherapy. This technique was extensively investigated in several prospective and retrospective studies.

In an initial prospective study, we evaluated the feasibility of HFPV and chest-wall motion reduction, by recruiting 15 healthy volunteers to undergo HFPV with three commercially available interfaces. For direct tumor motion immobilization, a second prospective study was performed in which with ten lung cancer patients underwent HFPV while imaged with high frame rate fluoroscopy. Diaphragm motion and image artifacts were quantified in a prospective study of a healthy volunteer …

Usability Engineering Of A Privacy-Aware Compliance Tracking System, Parameswara Reddy Annapureddy

ETD Archive

Software is useful when it is able to provide useful information to the end user with minimum effort. This thesis is about usability improvements to a privacy-aware human motion tracking system for healthcare professionals. The original system has a number of usability issues: (1) Users need to wear a smartwatch, which will be used to connect to the system; (2) Data are stored in XML, comma-separated-value format which is very difficult to analyze; (3) Data are available only at the local computer and there is no easy way to access them remotely via a Web or mobile interface; (4) Analysis …

Untapped Potential Of Clinical Text For Opioid Surveillance, Amy L. Olex, Tamas Gal, Majid Afshar, Dmitriy Dligach, Niranjan Karnik, Travis Oakes, Brihat Sharma, Meng Xie, Bridget T. Mcinnes, Julian Solway, Abel Kho, William Cramer, F. Gerard Moeller

Wright Center for Clinical and Translational Research Works

Accurate surveillance is needed to combat the growing opioid epidemic. To investigate the potential volume of missed opioid overdoses, we compare overdose encounters identified by ICD-10-CM codes and an NLP pipeline from two different medical systems. Our results show that the NLP pipeline identified a larger percentage of OOD encounters than ICD-10-CM codes. Thus, incorporating sophisticated NLP techniques into current diagnostic methods has the potential to improve surveillance on the incidence of opioid overdoses.

Identified Flying Objects: A Multidisciplinary Scientific Approach To The Ufo Phenomenon, Dr. Michael P. Masters

Interdisciplinary Arts and Sciences Books

Identified Flying Objects cautiously examines the premise that ‘UFOs’ & ‘Aliens’ are simply our distant human descendants, returning from the future to study their own hominin evolutionary past. This text challenges readers to consider new possibilities while cultivating conversations about our ever-evolving understanding of time & time travel.

The Relationship Between Rock Climbing And Total Body Strength And Endurance, Madison Gamma, Alexa Bruce, Madison Massey, Ashlee Cordero

The Corinthian

Purpose: The purpose of our study was to assess the relationship between muscular strength and endurance and time to complete a rock wall climb.

Methods: The researchers of this study tested 22 participants ranging from 19-25 years of age. An informed consent and a Physical Activity Readiness Questionnaire (PARQ+) form were completed by each individual before being cleared to participate in this study. Data collection was completed on two separate days. On the first day, participants completed four fitness tests which consisted of a hand grip strength assessment, a 10-repetition maximum (RM) leg press, a flexed arm hang test, and …

Santa Clara Magazine, Volume 61 Number 1, Winter 2019, Santa Clara University

Santa Clara Magazine

14 - IN THE BEGINNING An interview with Santa Clara University President Kevin O’Brien, S.J. on what's next and noticing what we swim in. Matt Morgan

22 - ADAM, EVE, AND THE APPLE If making—and appreciating—art makes us human, what happens when we get help making a masterpiece from something unhuman? Lauren Loftus.

28 - BEDROCK When faced with tough decisions, Santa Clara values stand as good guides, says Leon Panetta ’60, J.D. ’63. Leslie Griffy.

Thirty-Second Annual Symposium Of Trinity College Undergraduate Research, Trinity College, Hartford Connecticut

Science Symposia Abstracts

2019 annual volume of abstracts for science research projects conducted by students at Trinity College.

Research Day Abstracts 2018-2019

Student Research

No abstract provided.

A Complete Approach To Predict Biodistribution Of Nanomaterials Within Animal Species From In-Vitro Data, Edward Price

Electronic Theses and Dissertations

Smart drug-design for antibody and nanomaterial-based therapies allows for optimization of drug efficacy and more efficient early-stage pre-clinical trials. The ideal drug must display maximum efficacy at target tissue sites, but to track and predict distribution to these sites, one must have a mechanistic understanding of the kinetics involved with the individual cells of the tissue itself. This process can be tracked through biological simulations coupled with in-vitro approaches, which result in a rapid and efficient in-depth understanding of drug transport within tissue vasculature and cellular environment. As a result, it becomes possible to predict drug biodistribution within live animal …

Health Risk Tolerance As A Key Determinant Of (Un)Willingness To Behavior Change: Conceptualization And Scale Development, Hyoyeun Jun, Yan Jin

International Crisis and Risk Communication Conference

After the study of testing determinants of risk tolerance affecting information sharing, this study was conducted as a second step to actually develop the scale for risk tolerance. Firstly, this study followed qualitative steps, such as in-depth interview and focus group, to capture how public describes the situation when they are tolerating the risk, when they knew what the recommended behavior is to relieve the risk. Secondly, this study collected 1000 U.S. public sample for the survey questionnaire that are the items generated from the qualitative steps.

Evaluating The Impacts Of Antidepressant Use On The Risk Of Dementia, Ran Duan

Theses and Dissertations--Epidemiology and Biostatistics

Dementia is a clinical syndrome caused by neurodegeneration or cerebrovascular injury. Patients with dementia suffer from deterioration in memory, thinking, behavior and the ability to perform everyday activities. Since there are no cures or disease-modifying therapies for dementia, there is much interest in identifying modifiable risk factors that may help prevent or slow the progression of cognitive decline. Medications are a common focus of this type of research.

Importantly, according to a report from the Centers for Disease Control and Prevention (CDC), 19.1% of the population aged 60 and over report taking antidepressants during 2011-2014, and this number tends to …

Momentum 2019, Stephanie Clarke

Momentum

A journal of undergraduate research

Replacing Animal Tests To Improve Safety For Humans, Kathy Archibald, Robert Coleman, Tamara Drake

Application of Alternative Methods Collection

In this chapter, we propose a new, pragmatic approach that could accelerate the replacement of most, if not all, regulatory animal tests with superior tests based on human biology. We also propose that changes to the requirements for safety testing, issued by the us Food and Drug Administration (fda), must be made in order to enable the use of superior new tests, which are currently disadvantaged by the outdated language of the regulations. But first, it is imperative to establish some level of understanding of the efficacy of existing animal-based methods in order to know whether any possible replacement is …

Pharmacodynamics Of Monoamine Transporter Releasing Agents And Reuptake Inhibitors, Alexa Holloway

Theses and Dissertations

Ligands of the human monoamine transporters encompass a wide range of both illicit and therapeutic drugs that act upon neural circuitry related to reward, motivation, and the processing of salient stimuli. The present study utilizes two methods for analyzing transporter substrates and inhibitors in order to characterize activity and assess potency. The first measures transient changes in intracellular calcium as a surrogate for transporter activity by harnessing the electrical coupling of monoamine transporters and L-type calcium channels. This is used to analyze novel chimera of the strong hDAT inhibitors methylphenidate and ��-PPP in order to assess the contribution of specific …

Risk Attitudes Affect Livestock Biosecurity Decisions With Ramifications For Disease Control In A Simulated Production System, Gabriela Bucini, Scott C. Merrill, Eric Clark, Susan M. Moegenburg, Asim Zia, Christopher J. Koliba, Serge Wiltshire, Luke Trinity, Julia M. Smith

College of Agriculture and Life Sciences Faculty Publications

HOG producers' operational decisions can be informed by an awareness of risks associated with emergent and endemic diseases. Outbreaks of porcine epidemic diarrhea virus (PEDv) have been re-occurring every year since the first onset in 2013 with substantial losses across the hog production supply chain. Interestingly, a decreasing trend in PEDv incidence is visible. We assert that changes in human behaviors may underlie this trend. Disease prevention using biosecurity practices is used to minimize risk of infection but its efficacy is conditional on human behavior and risk attitude. Standard epidemiological models bring important insights into disease dynamics but have limited …

Willingness To Comply With Biosecurity In Livestock Facilities: Evidence From Experimental Simulations, Scott C. Merrill, Susan Moegenburg, Christopher J. Koliba, Asim Zia, Luke Trinity, Eric Clark, Gabriela Bucini, Serge Wiltshire, Timothy Sellnow, Deanna Sellnow, Julia M. Smith

College of Agriculture and Life Sciences Faculty Publications

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Disease in U.S. animal livestock industries annually costs over a billion dollars. Adoption and compliance with biosecurity practices is necessary to successfully reduce the risk of disease introduction or spread. Yet, a variety …

Tolle Lege Works Published In 2019, Mcquade Library

Bibliographies

Compiled Tolle Lege bibliography of works published by members of the Merrimack College Community in 2019. Works include journal articles, books, book chapters, conference presentations and posters, and more.

From The Anthropocene To Mutual Thriving: An Agenda For Higher Education In The Ecozoic, Ivan Vargas Roncancio, Leah Temper, Joshua Sterlin, Nina L. Smolyar, Shaun Sellers, Maya Moore, Rigo Melgar-Melgar

College of Arts and Sciences Faculty Publications

Higher education in the global North, and exported elsewhere, is complicit in driving the planet's socio-ecological crises by teaching how to most effectively marginalize and plunder Earth and human communities. As students and activists within the academic system, we take a firm stand to arrest this cycle, and to redirect education toward teaching how to create conditions for all life to thrive. In this paper, we articulate a research and education agenda for co-constructing knowledge and wisdom, and propose shifts in the 'ologies from the current, destructive modes to intended regenerative counterparts. We offer to shift from an ontology of …

Isolation Of Rabies Virus From The Salivary Glands Of Wild And Domestic Carnivores During A Skunk Rabies Epizootic, Cornell University Jimenez, Terry R. Spraker, Jessica Anderson, Richard Bowen, Amy Gilbert

United States Department of Agriculture Wildlife Services: Staff Publications

Rabies is a fatal zoonotic disease of global importance. Rabies virus is shed in the saliva of infected hosts and is primarily transmitted through bite contact. Canine rabies has been eliminated from the US, but wildlife constitutes more than 90% of the reported cases of animal rabies in the US each year. In the US, several wild carnivore species are reservoirs of distinct variants of rabies virus (RV). After decades of apparent absence, the south-central skunk (SCSK) RV variant was detected in Colorado in 2007 and resulted in a large-scale epizootic in striped skunk (Mephitis mephitis) populations in northern Colorado …

Movement Responses Inform Effectiveness And Consequences Of Baiting Wild Pigs For Population Control, Nathan P. Snow, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) damage agricultural and natural resources throughout their nearly global distribution. Subsequently, population control activities (e.g., trapping, shooting, or toxic baiting) frequently involve the deployment of bait to attract wild pigs. A better understanding of how wild pigs respond to bait sites can help maximize efficiency of baiting programs and identify any potential pitfalls. We examined the movement behaviors of 68 wild pigs during three stages of intensive baiting programs (i.e., 15 days each: prior, during, and post baiting) spread across two distinct study areas in southern and northern Texas, USA. We found that bait sites needed …

Multivariate Statistical Methodologies Used In In-Vitro Raman Spectroscopy: Simulations And Applications For Drug And Nanoparticle Interactions, Mark Edward Keating

Doctoral

Raman spectroscopy is a growing technology in the fields of in-vitro drug and nanoparticle screening. The label free capability provided by vibrational spectroscopy, as well as the ability of the technique to probe the chemical nature of samples, makes it a good candidate for use in these fields. Crucial to the progress of these methods is the development and validation of robust and accurate multivariate statistical analysis protocols. In this thesis, both established and novel methods are examined using both real and simulated datasets. In particular, simulated datasets are used to validate and assess the accuracy of these methods in …

Determining The Presence Of Bpa In Vaping Mouthpieces, Jessica Cook-Snelgrove

Honors Theses

As more and more teenagers and young adults begin to vape, whether it be to quit smoking or to look cool, there is a potential for them to be exposed to bisphenol-A (BPA) through the plastic mouthpieces on the vapes. BPA is a molecule that is added in many plastics to make the plastic harder (Houlihan, Lunder, & Jacob, 2008). Unfortunately, BPA is structurally similar to estrogen as can be seen in Figure 1. The closeness of the structures can cause issues in humans such as cardiovascular disease, diabetes, and reproductive problems (Houlihan, Lunder, & Jacob, 2008). In recent years, …

Keynote Address And Meeting Program, Academy Editors

Journal of the Arkansas Academy of Science

No abstract provided.