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Full-Text Articles in Physical Sciences and Mathematics

Water Quality Dynamics Of Low-Gradient, Headwater Streams In A Timber-Industry Dominated Watershed In Louisiana, Abram Atys Dasilva Jan 2012

Water Quality Dynamics Of Low-Gradient, Headwater Streams In A Timber-Industry Dominated Watershed In Louisiana, Abram Atys Dasilva

LSU Master's Theses

Timber harvesting can degrade the quality of adjacent water bodies, an important concern for Louisiana, nearly 50% of which is forested, and in which the forest industry is the second-largest manufacturing employer. To protect valuable freshwater resources in Louisiana, a manual of best management practices (BMPs) was published in 2000 describing techniques for limiting forestry-caused water quality degradation. While these BMPs are widely implemented, their effectiveness in protecting water quality is largely unknown. To determine the effectiveness of these BMPs, this thesis research conducted three studies to address timber harvest BMP effectiveness on protection of stream dissolved oxygen, metabolism, and …


Detection And Characterization Of Vibrio Vulnificus And Vibrio Parahaemolyticus Isolates: Pentaplex Pcr Assay And Its Application., Nabanita Bhattacharyya Jan 2012

Detection And Characterization Of Vibrio Vulnificus And Vibrio Parahaemolyticus Isolates: Pentaplex Pcr Assay And Its Application., Nabanita Bhattacharyya

LSU Master's Theses

The aim of the study was to develop an effective multiplex PCR protocol for simultaneous, rapid detection and characterization of two potential pathogens, Vibrio vulnificus and Vibrio parahaemolyticus and to enumerate their abundance in the environment. A Pentaplex PCR (pPCR) assay condition was developed with a combination of two species- and three pathogenic- specific PCR primer sets to simultaneously detect and characterize bacterial isolates for virulent/ non- virulent strains of V. parahaemolyticus and V. vulnificus. The pPCR assay was validated by three methods. First pPCR was tested on 300 bacterial isolates comprising of 7 reference strains, 117 V. vulnificus, 30 …


Soil Accretion And Organic Carbon Accumulation In The Tidal Salt Marshes Of The Liaohe Delta, China, Nicholas Lawrence Yuknis Jan 2012

Soil Accretion And Organic Carbon Accumulation In The Tidal Salt Marshes Of The Liaohe Delta, China, Nicholas Lawrence Yuknis

LSU Master's Theses

Wetlands provide a multitude of functions to people and ecosystems and because of this, maintaining or increasing wetland area is important. Wetlands can be significant carbon sinks because of the relatively high primary productivity and slow decomposition of organic matter in these systems and can thus impact the global carbon cycle and potentially climate change. The marshes of the Liaohe Delta are the largest in Liaoning Province; however, very little research has been done in this area to determine the rate of accretion and organic carbon sequestration in the salt marshes there. By using Cs-137 and Pb-210 dating techniques and …


Utilization Of Machined Skin Collimation For Electron Therapy, Ryan Kenneth Posey Jan 2012

Utilization Of Machined Skin Collimation For Electron Therapy, Ryan Kenneth Posey

LSU Master's Theses

Purpose: Electron skin collimation (eSC) sharpens the penumbra, allowing electron radiotherapy of cancers while minimizing dose to adjacent normal tissue, e.g., protecting the eye in treating eyelid and nose cancers. This study assessed the clinical potential for milled brass eSCs to replace manually-fabricated lead or Cerrobend eSCs. Methods: Aim one was to measure the influence of eSC edge angle on the dose distributions, which were measured under edges of lead eSCs for combinations of three bevel (0°,±10°) and five gantry (0°,±3°,±5°) angles for a 6-MeV beam. Aim two was to validate the dimensional conformity of three machined brass eSCs, one …


Spatial Variability In Response Of Deltaic Baldcypress Forests To Hydrology And Climate, Som Bahadur Bohora Jan 2012

Spatial Variability In Response Of Deltaic Baldcypress Forests To Hydrology And Climate, Som Bahadur Bohora

LSU Master's Theses

Changing hydrology and climate have significantly altered ecologically significant cypress-tupelo forests in the deltaic plain of the Mississippi River. Though local responses of these swamps to hydrology are relatively well understood, broad-scale evaluation of ecological response differences of deltaic wetland forests has been limited by the lack of site-specific coupled hydrologic and ecological data. Baldcypress tree-rings from twenty-two sites were used to examine the responses of coastal swamp forests throughout the deltaic plain to environmental variables. The responses of these forests to environmental variables varied spatially; however, broad-scale measures of deltaic hydrology were more correlated with tree radial growth than …


Evaluation Of The Depositional Environment Of The Eagle Ford Formation Using Well Log, Seismic, And Core Data In The Hawkville Trough, Lasalle And Mcmullen Counties, South Texas, Zachary Paul Hendershott Jan 2012

Evaluation Of The Depositional Environment Of The Eagle Ford Formation Using Well Log, Seismic, And Core Data In The Hawkville Trough, Lasalle And Mcmullen Counties, South Texas, Zachary Paul Hendershott

LSU Master's Theses

The Upper Cretaceous Eagle Ford Formation of South Texas records a mixed siliciclastic/carbonate depositional environment across the Late Cretaceous Platform of the Gulf of Mexico. During the Late Cretaceous, LaSalle and McMullen Counties was part of the Hawkville Trough, a wedged shaped region between the Edwards and Sligo carbonate reefs. Well logs from 21 wells and seismic data were used to construct structure and isopach maps of the Eagle Ford Formation throughout the Hawkville Trough. Only the unconformable bottom (Buda-Eagle Ford) and top (Eagle Ford-Austin Chalk) boundaries plus the conformable boundary between the upper and lower Eagle Ford can be …


Sediment Flux & Fate For A Large-Scale Diversion: The 2011 Mississippi River Flood, The Bonnet Carré Spillway, And The Implications For Coastal Restoration In South Louisiana, Jeffrey Bryant Fabre Jan 2012

Sediment Flux & Fate For A Large-Scale Diversion: The 2011 Mississippi River Flood, The Bonnet Carré Spillway, And The Implications For Coastal Restoration In South Louisiana, Jeffrey Bryant Fabre

LSU Master's Theses

Dams and hardened levees built by European settlers in the last two centuries have degraded or stopped the cyclic sedimentation patterns that built the Mississippi River Delta Plain over the past 7,000-8,000 years. Man-made diversions of sediment-laden river water along the lower Mississippi, operated during high water periods, are being considered as a primary mechanism for combating the land loss induced by rising sea-level and these modern controls. To date, no study has documented the sediment-delivery potential of a large man-made river diversion during a single high water event. In the spring of 2011, historic flooding on the Mississippi River …


The Feasibility Of Using Megavoltage Ct For The Treatment Planning Of Hdr Cervical Brachytherapy With Shielded Tandem And Ovoid Applicators, Jeffrey Roger Kemp Jan 2012

The Feasibility Of Using Megavoltage Ct For The Treatment Planning Of Hdr Cervical Brachytherapy With Shielded Tandem And Ovoid Applicators, Jeffrey Roger Kemp

LSU Master's Theses

Purpose/Objectives: A drawbackof tandem and ovoid (T&O) ICBT is exposure of the posterior bladder and anterior rectal walls to relatively high isodoses. To mitigate this effect, intra-ovoid shielding may be used to reducedose to these OARs. However, metal artifactspresent in images acquired via kVCT make anatomy segmentation and catheter localization difficult for the purpose of 3D treatment planning. We present a method that combines MVCT-based imaging and applicator modeling to increase the quality of 3D treatment plans for shielded T&O ICBT. Materials/Methods: Using Oncentra’s TPS, 9 participants from multiple institutions performed organ segmentation and catheter reconstruction for KVCT and MVCT …


The Ecological Impact Of The Deepwater Horizon Oil Spill On Vibrio Parahaemolyticus Type Iii Secretion System And The Vibrio Community, Erica L. Stephens Jan 2012

The Ecological Impact Of The Deepwater Horizon Oil Spill On Vibrio Parahaemolyticus Type Iii Secretion System And The Vibrio Community, Erica L. Stephens

LSU Master's Theses

Vibrio spp are gram-negative, halophilic bacteria that naturally occur in both shallow coastal waters and parts of the deepest oceans and are a known human pathogen. There have not been extensive studies that analyze an oil spill as an environmental stressor to vibrios. If there were to be an increase in the pathogenicity of vibrios it would be a cause for human health concern. The purpose of the two studies presented was to determine changes in the pathogenicity of V. parahaemolyticus pathogenicity and the vibrio community, with respect to V. cholera, V. parahaemolyticus, and V. vulnificus. The results showed that …


Effect Of Hurricane Forward Speed And Approach Angle On Coastal Storm Surge, Chenguang Zhang Jan 2012

Effect Of Hurricane Forward Speed And Approach Angle On Coastal Storm Surge, Chenguang Zhang

LSU Master's Theses

Hurricane induced storm surges can be destructive and can pose great threats to coastal communities. There are many factors affecting storm surges, including the hurricane’s intensity and movement, the bathymetry and coastline of affected area, rainfall, tide, and river stage. This thesis focuses on the effects of hurricane approach angle (θ) and forward speed (U), which together describe the hurricane’s motion. The problem is approached through several numerical experiments with increasing complexity. First are some process studies of long wave propagations near the coast, followed by examination of the generation of long waves by a traveling wind field. The central …


An Extensible And Scalable Pilot-Mapreduce Framework For Data Intensive Applications On Distributed Cyberinfrastructure, Pradeep Kumar Mantha Jan 2012

An Extensible And Scalable Pilot-Mapreduce Framework For Data Intensive Applications On Distributed Cyberinfrastructure, Pradeep Kumar Mantha

LSU Master's Theses

The volume and complexity of data that must be analyzed in scientific applications is increasing exponentially. Often, this data is distributed; thus, the ability to analyze data by localizing it will yield limited returns. Therefore, an efficient processing of large distributed datasets is required, whilst ideally not introducing fundamentally new programming models or methods. For example, extending MapReduce - a proven effective programming model for processing large datasets, to work more effectively on distributed data and on different infrastructure (such as non-Hadoop, general-purpose clusters) is desirable. We posit that this can be achieved with an effective and efficient runtime environment …


The Effects Of Regulatory Uncertainty On The U.S. Acid Rain Program, Narendra Paramanand Jan 2012

The Effects Of Regulatory Uncertainty On The U.S. Acid Rain Program, Narendra Paramanand

LSU Master's Theses

Title IV of the Clean Air Act Amendments of 1990 (CAAA 1990) created the first large scale cap-and-trade program as a means to control acid rain in the United States. The program regulated the emission of sulfur dioxide (SO2) and nitrous oxide (NOx) in the atmosphere—the precursor to acid rain (formerly acid deposition). Economists have long argued for the use of market-based incentive approach as oppose to the traditional command-and-control methods for its ability to be efficient and cost-effective. Title IV went into full effect in 1995 and has been lauded among one of the most successful regulation as it …


Ecology Of Rusty Blackbirds Wintering In Louisiana: Seasonal Trends, Flock Composition And Habitat Associations, Emma Elizabeth Deleon Jan 2012

Ecology Of Rusty Blackbirds Wintering In Louisiana: Seasonal Trends, Flock Composition And Habitat Associations, Emma Elizabeth Deleon

LSU Master's Theses

With an estimated population loss of at least 5% annually and a 100-year history of decline, Rusty Blackbirds (Euphagus carolinus) are one of the fastest declining bird species in North America. Determining cause of decline is important, both for conserving the species and for identifying threats to the wooded wetland ecosystems they use on their boreal breeding-grounds and their wintering-grounds in the southeastern United States. One hypothesis is that loss of wintering ground habitat, possibly in conjunction with competition or disease, is causing Rusty Blackbird decline. To determine contribution of wintering ground conditions, it is important to understand the behaviors …


Comprehensive Planning And Resilience: A Study Of Louisiana Parishes After Hurricane Katrina, Mary Paille Jan 2012

Comprehensive Planning And Resilience: A Study Of Louisiana Parishes After Hurricane Katrina, Mary Paille

LSU Master's Theses

When hurricanes Katrina and Rita hit in 2005, widespread devastation was felt in over half of the parishes in the state. More than 200,000 homes were damaged and more than 1500 people lost their lives. During this transitionary period, communities were vulnerable and looked for rebuilding leadership. As part of a post-catastrophe resilience movement, the Louisiana Recovery Authority formulated a 50-year regional plan for recovering south Louisiana called Louisiana Speaks. This planning process opened up an opportunity to reach those communities that otherwise may not have considered planning or how it could help them prepare for future events. This places …


The Distribution And Diversity Of Functional Gene Pathways Controlling Sulfur Speciation In Lower Kane Cave, Wyoming, Audrey Tarlton Paterson Jan 2012

The Distribution And Diversity Of Functional Gene Pathways Controlling Sulfur Speciation In Lower Kane Cave, Wyoming, Audrey Tarlton Paterson

LSU Master's Theses

Defining the linkages between microbial metabolic activity and environmental geochemistry can be used to understand how carbonate dissolution in sulfidic karst systems proceeds. In Lower Kane Cave, Wyoming, sulfidic springs support taxonomically distinct microbial communities that are also associated with putative sulfur metabolisms (e.g., oxidation of reduced sulfur compounds, sulfate reduction, and sulfur disproportionation) that influence carbonate dissolution. The distribution of diverse microbial groups in other terrestrial subsurface environments is not well understood, making Lower Kane Cave ideal for study. Molecular genetics techniques were used to uncover diversity of 16S rRNA genes and bacterial soxB and aprA functional genes. Aqueous …


Writing In Geometry With The Common Core State Standards: Developing Mathematical Thinkers, Yvonne Mariki Chimwaza Jan 2012

Writing In Geometry With The Common Core State Standards: Developing Mathematical Thinkers, Yvonne Mariki Chimwaza

LSU Master's Theses

The newly released Common Core State Standards (CCSS) were adopted with the goal in mind that in the future our students will leave high school ready and better prepared for college and careers. In particular, the CCSS insists that a faithful implementation of the eight Standard of Mathematical Practices will lead to a generation of mathematical thinkers who have learned how to read, write, model, reason, and solve problems in mathematical terms. Unfortunately, at present, my students and others do not know how to write and reason mathematically. By way of this thesis, I searched for ways to help forty-five …


Evaluation Of Pleistocene To Holocene (Mis 5 To 1) Climatic Changes In Southwestern Black Sea: A Palynological Study Of Dsdp Site 380, Shannon Ferguson Jan 2012

Evaluation Of Pleistocene To Holocene (Mis 5 To 1) Climatic Changes In Southwestern Black Sea: A Palynological Study Of Dsdp Site 380, Shannon Ferguson

LSU Master's Theses

Site 380 of the Deep Sea Drilling Program [DSDP] is located in the southwestern region of the Black Sea (42°05.94’, 29°36.82’E). The location of this site is important due to its proximity to the narrow Bosphorus strait, the only present-day connection between the Black Sea and the Aegean Sea. Drops in eustatic sea level during extensive Pleistocene glacial episodes disconnected the Black Sea from the Aegean Sea at the Bosphorus strait, creating a change from brackish to freshwater within the Black Sea during these intervals. Organic-walled phytoplankton (dinoflagellate cysts, acritarchs, and fresh-water algae) are investigated to better constrain these glacio-eustatic …


Effects Of Varying Land Use On Headwater Stream Fish Assemblages And In-Stream Habitats In Southwestern Louisiana, Alexandra Marie Fitzgerald Jan 2012

Effects Of Varying Land Use On Headwater Stream Fish Assemblages And In-Stream Habitats In Southwestern Louisiana, Alexandra Marie Fitzgerald

LSU Master's Theses

Although watershed land use effects on in-stream fish habitat and fish-macrohabitat associations have been widely studied in the past, low-gradient, coastal Louisiana streams have been poorly described in the literature. In this thesis, I report the results of a two-year study exploring relationships among regional land use, in-stream physical habitat, and headwater stream fish assemblages. In chapter two, I examined in-stream habitat variables such as depth, flow, and substrate combined with three-pass electrofishing depletion estimates at thirteen 100-m stream sites. I used a combination of principle component analysis and structural equation modeling to determine if trends were present in the …


Determining Impact: Using Formative Evaluation In A Professional Development Program For Teachers Of Mathematics And Science, Tiah B. Alphonso Jan 2012

Determining Impact: Using Formative Evaluation In A Professional Development Program For Teachers Of Mathematics And Science, Tiah B. Alphonso

LSU Master's Theses

The purpose of this study was to evaluate a professional development (PD) program for middle and high school teachers of mathematics and science which is funded by a $5 million National Science Foundation grant. The evaluation was internal and formative in nature and took place in two separate phases. The focus of the evaluation was not only on program improvement but also to extend the body of existing knowledge in the area of teacher professional development. Both the needs of project stakeholders and the findings of previous research in the areas of professional development and program evaluation were drawn on …


Ensemble Methods For Malware Diagnosis Based On One-Class Svms, Xing An Jan 2012

Ensemble Methods For Malware Diagnosis Based On One-Class Svms, Xing An

LSU Master's Theses

Malware diagnosis is one of today’s most popular topics of machine learning. Instead of simply applying all the classical classification algorithms to the problem and claim the highest accuracy as the result of prediction, which is the typical approach adopted by studies of this kind, we stick to the Support Vector Machine (SVM) classifier and based on our observation of some principles of learning, characteristics of statistics and the behavior of SVM, we employed a number of the potential preprocessing or ensemble methods including rescaling, bagging and clustering that may enhance the performance to the classical algorithm. We implemented the …


Technology In The Classroom: Effect Of Student Blogging On Learning Gains In A High School Classroom, Mandy Lynn Lebourgeois Jan 2012

Technology In The Classroom: Effect Of Student Blogging On Learning Gains In A High School Classroom, Mandy Lynn Lebourgeois

LSU Master's Theses

This study explores the benefits of student use of web logs (blogs) in a high school Biology classroom. Students were assigned to blog by answering questions on topics from the Miller and Levine (2010) Biology textbook, which correlated to the Louisiana Grade-Level Expectations (GLEs), benchmarks that should be familiar to students at the end of a course. Raw gains (from pretest to posttest) were compared for the study group of 124 ninth and tenth grade students to determine if blogging increased student learning gains by increasing student accountability. The Louisiana Enhanced Assessment of Grade-Level Expectations (EAGLE) test bank was used …


A 2-D, Variable-Density Numerical Model Of Subsurface Fluid Flow Through The Edwards Aquifer, New Braunfels, Tx: Mechanisms Inhibiting Flow Across The Freshwater/Saline-Water Interface, Scott Ryan Tipple Jan 2012

A 2-D, Variable-Density Numerical Model Of Subsurface Fluid Flow Through The Edwards Aquifer, New Braunfels, Tx: Mechanisms Inhibiting Flow Across The Freshwater/Saline-Water Interface, Scott Ryan Tipple

LSU Master's Theses

The Edwards aquifer in south-central Texas, U.S., composed of faulted carbonate bedrock, contains freshwater and saline water. In aquifers that are used as a source of drinking water and that contain fresh and saline waters, saline-water intrusion can result in degradation of water quality. Yet, in the Edwards aquifer, limited saline-water intrusion has occurred. The questions addressed are “Why is the saline-water intrusion less than expected,” and “Is there a trigger that will result in saline-water intrusion into the freshwater reservoir?” Three hypotheses were tested. One: an extremely saline water density might prevent mixing across the interface. Two: faults could …


The Effects Of Teaching Style On Student Learning Of Dna, Rebecca Branton Jan 2012

The Effects Of Teaching Style On Student Learning Of Dna, Rebecca Branton

LSU Master's Theses

New methods and practices are constantly being introduced in education due to new research that emerges as a push for student achievement increases. We as teachers must adapt to these new ideas to ensure student success. This study was completed to determine if inquiry-based teaching methods would be more beneficial in learning Biology concepts than traditional lecture instruction. The students were divided into two groups, which differed based solely on their instructional format. The activities involved in this study included inquiry-based activities and traditional activities, i.e. PowerPoints, guided notes, experiments and worksheets. The participants in this study, students in a …


The Effects Of Oyster Harvest On Resident Oyster Reef Communities And Reef Structure In Coastal Louisiana Proper, Steven Lee Beck Jan 2012

The Effects Of Oyster Harvest On Resident Oyster Reef Communities And Reef Structure In Coastal Louisiana Proper, Steven Lee Beck

LSU Master's Theses

Harvest of the eastern oyster (Crassostrea virginica) is a primary contributor to oyster reef habitat disturbance in the northern Gulf of Mexico. The impacts of oyster dredging on reef substrate and resident fauna have not been thoroughly examined on the extensive sub-tidal oyster reefs of Louisiana. Several reef structure and resident community metrics were compared on unharvested and harvested reefs during the spring, summer, and fall of 2010. Unharvested reefs had higher amounts of oyster clusters, solid reef substrate, and more large oysters, while harvested reefs had higher amounts of loose shell, mixed shell/mud substrate, and elevated chlorophyll-a levels. Overall, …


Minimizing Epicormic Branch Formation On Louisiana Leading Commercial Bottomland Red Oaks, Denton William Culpepper Jan 2012

Minimizing Epicormic Branch Formation On Louisiana Leading Commercial Bottomland Red Oaks, Denton William Culpepper

LSU Master's Theses

The goal of this study was to isolate biological, natural, and silvicultural factors that encourage the production of epicormic branches on bottomland red oak saw-timber trees and to use this information to help landowners and forest managers reduce epicormic branches in stands dominated by bottomland red oaks. Quercus nigra, Q. pagoda, Q. phellos, and Q. texana, the most common commercial bottomland red oaks in Louisiana, were evaluated in this study. Both qualitative and quantitative variables were assessed in this study. The following attributes were collected from each sample tree and primary competitor: site class, basal area, crown class DBH, presence …


The New Stochastic Integral And Anticipating Stochastic Differential Equations, Benedykt Szozda Jan 2012

The New Stochastic Integral And Anticipating Stochastic Differential Equations, Benedykt Szozda

LSU Doctoral Dissertations

In this work, we develop further the theory of stochastic integration of adapted and instantly independent stochastic processes started by Wided Ayed and Hui-Hsiung Kuo in [1,2]. We provide a first counterpart to the Itô isometry that accounts for both adapted and instantly independent processes. We also present several Itô formulas for the new stochastic integral. Finally, we apply the new Itô formula to solve a linear stochastic differential equations with anticipating initial conditions.


Sources Of Salinization Of The Baton Rouge Aquifer System: Southeastern Louisiana, Callie Elizabeth Anderson Jan 2012

Sources Of Salinization Of The Baton Rouge Aquifer System: Southeastern Louisiana, Callie Elizabeth Anderson

LSU Master's Theses

A major environmental and economic problem confronting the Baton Rouge area, southeastern Louisiana, is the progressive salinization of the Baton Rouge aquifer system, an important source of fresh municipal and industrial groundwater. The Baton Rouge fault marks a boundary between fresh groundwaters to the north and predominately brackish waters to the south. Determining the permeability architecture of the fault is an essential component of modeling groundwater flow and saline contamination north of the fault. Understanding the sources and pathways of the saline waters that are migrating into the freshwater sands north of the fault is thus essential in helping to …


Habitat- And Region-Specific Reproductive Biology Of Female Red Snapper (Lutjanus Campechanus) In The Gulf Of Mexico, Dannielle Helen Kulaw Jan 2012

Habitat- And Region-Specific Reproductive Biology Of Female Red Snapper (Lutjanus Campechanus) In The Gulf Of Mexico, Dannielle Helen Kulaw

LSU Master's Theses

This study compares reproductive biology estimates of female red snapper among three habitat types (natural shelf-edge banks, standing petroleum platforms and toppled petroleum platforms) and among six regions in the Gulf of Mexico (central Florida, northwest Florida, Alabama, Louisiana, north Texas and south Texas). In both studies, batch fecundity and spawning frequency increased with length, weight and age, and batch fecundity was best correlated with maternal length. Gulf-wide, 75% maturity was achieved by age-3 and 100% maturity was reached by age-8. Sizes- and ages-at-maturity differed among habitat types and regions. Females from natural habitat reached 50% maturity the slowest (age-5, …


Improving Middle School Math Achievement Using A Web-Based Program And Extended Written Tasks, Sarah Claire Dyer Jan 2012

Improving Middle School Math Achievement Using A Web-Based Program And Extended Written Tasks, Sarah Claire Dyer

LSU Master's Theses

This thesis outlines a dual-intensity approach using a web-based program, MyMathLab, for procedural fluency and, in parallel, extended written tasks for helping students improve their reasoning skills, to learn to use multiple representations, and securing mathematical knowledge. The new Common Core State Standards have increased expectations and achievement goals at all grade levels, the required changes being most significant at earlier grade levels (in elementary and middle schools). It is my assertion that a combined approach, one that encompasses both procedure-oriented practice for fluency and extended written tasks designed to stretch thinking and reasoning is needed to meet these goals.


Investigations Into The Role Of Humic Acid In Biomembrane Permeability And The Effects Of Naturally Formed Gold And Silver Nanoparticles On These Interactions, Loice Marklyne Ojwang Jan 2012

Investigations Into The Role Of Humic Acid In Biomembrane Permeability And The Effects Of Naturally Formed Gold And Silver Nanoparticles On These Interactions, Loice Marklyne Ojwang

LSU Doctoral Dissertations

The mechanism and kinetics of the interaction of humic acids (HAs) with a 1-palmitoyl-2-oleoyl-Sn-glycero-3-phosphatidylcholine (POPC) large unilamellar vesicle (LUV) model biomembrane system were studied by fluorescence spectroscopy. All three HAs studied induced a perturbation to the biomembrane bilayer structure at pH 4.8. Concentration dependence studies revealed that biomembrane perturbation increased with increasing HA concentrations for Suwannee River HA (SWHA) from 0 to 20 mg C/L. For both Leonardite HA (LAHA) and Florida Peat HA (FPHA) aggregation influenced biomembrane perturbation at concentrations above 5 and 7.5 mg C/L, respectively. Temperature studies over the environmentally relevant ranges of 10 to 30 °C …