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Full-Text Articles in Physical Sciences and Mathematics

Contributions Of The United States Department Of Agriculture Natural Resources Conservation Service To Conserving Grasslands On Private Lands In The United States, L. Peter Heard Mar 2023

Contributions Of The United States Department Of Agriculture Natural Resources Conservation Service To Conserving Grasslands On Private Lands In The United States, L. Peter Heard

IGC Proceedings (1993-2023)

The future of biodiversity in the USA is tied inseparably to activities taking place on private lands. Agriculture is by far the most important user of these lands, with about 50% or 900M acres managed as private cropland, grassland or rangeland. Decisions made by America’s farmers and ranchers directly affect grasslands and their impact on food supply, biodiversity, soil protection and water quality. Agricultural programs and policies in the USA have had a large influence on the choices available to farmers and ranchers in land management. Since the 1930s, USDA’s Natural Resource Conservation Service (NRCS) has been working with farmers, …


Effect Of Mixed And Sequential Grazing By Cattle And Sheep Of Upland Permanent Pasture On Liveweight Gain, M. D. Fraser, J. E. Vale, J. G. Evans Mar 2023

Effect Of Mixed And Sequential Grazing By Cattle And Sheep Of Upland Permanent Pasture On Liveweight Gain, M. D. Fraser, J. E. Vale, J. G. Evans

IGC Proceedings (1993-2023)

Previous work has shown benefits of sequential grazing by cattle and sheep, with superior liveweight gains being recorded for lambs grazing swards previously grazed by cattle. A preliminary study of the effects of mixed sheep and cattle grazing also suggested that more extensively grazed swards offer scope for complementary grazing between sheep and cattle. The aim of this experiment was to directly compare these two approaches of integrating the grazing of cattle and sheep.


Effect On Sward Botanical Composition Of Mixed And Sequential Grazing By Cattle And Sheep Of Upland Permanent Pasture In The Uk, J. E. Vale, M. D. Fraser, J. G. Evans Mar 2023

Effect On Sward Botanical Composition Of Mixed And Sequential Grazing By Cattle And Sheep Of Upland Permanent Pasture In The Uk, J. E. Vale, M. D. Fraser, J. G. Evans

IGC Proceedings (1993-2023)

Previous work has shown benefits of sequential grazing by cattle and sheep, with superior liveweight gains being recorded for lambs grazing swards previously grazed by cattle. A preliminary study of the effects of mixed sheep and cattle grazing also suggested that more extensively grazed swards offer scope for complementary grazing between sheep and cattle. The aim of this experiment was to directly compare these two approaches of integrating the grazing of cattle and sheep.


Effects Of Breed And Stage Of Growing Season On The Metabolic Profile Of Sheep Grazing Moorland, V. J. Theobald, M. D. Fraser, J. M. Moorby Mar 2023

Effects Of Breed And Stage Of Growing Season On The Metabolic Profile Of Sheep Grazing Moorland, V. J. Theobald, M. D. Fraser, J. M. Moorby

IGC Proceedings (1993-2023)

Previous studies have shown that the diet of sheep grazing heather moorland is affected by season (Grant et al., 1987) and the proportion of Calluna vulgaris cover (Osoro et al., 2000). In order to investigate the impact of these factors on the associated nutrient supply of animals grazing heathland, blood samples were taken to monitor the metabolic status of different breeds of sheep at different stages of the growing season when grazing sites with different proportions of heather cover.


Characterising The Fermentation Capabilities Of Gut Microbial Populations From Different Breeds Of Cattle And Sheep Grazing Heathland, D. R. Davies, M. D. Fraser, V. J. Theobald, E. L. Bakewell Mar 2023

Characterising The Fermentation Capabilities Of Gut Microbial Populations From Different Breeds Of Cattle And Sheep Grazing Heathland, D. R. Davies, M. D. Fraser, V. J. Theobald, E. L. Bakewell

IGC Proceedings (1993-2023)

Previous studies have demonstrated differences in the diet composition of sheep and cattle when grazing heather moorland, and such differences may in turn lead to differences in rumen fermentation characteristics and associated adaptation to diet. To investigate this further an in vitro gas production experiment was conducted using inocula from different breeds of cattle and sheep grazing heathland.


The Performance Of Cattle On Lowland Species-Rich Neutral Grassland At Three Contrasting Grazing Pressures, B. A. Griffith, J. R.B. Tallowin Mar 2023

The Performance Of Cattle On Lowland Species-Rich Neutral Grassland At Three Contrasting Grazing Pressures, B. A. Griffith, J. R.B. Tallowin

IGC Proceedings (1993-2023)

Grazing is an essential management practice for maintaining the nature conservation value of lowland semi-natural neutral grassland to control succession and create different faunal habitats via structural heterogeneity within the pasture (Duffey et al., 1974). However, there is a paucity of information on what would constitute a sustainable grazing intensity that will deliver the wildlife objectives and what the consequences of this management would be on growth rate of livestock and overall pasture output. An experiment was designed to quantify the ecological and agronomic consequences of imposing different grazing intensities on species-rich neutral grassland. The results will provide sward-based …


Nutritional Value Of Pasture Forage For Sheep In Krkonoše National Park, P. Homolka Mar 2023

Nutritional Value Of Pasture Forage For Sheep In Krkonoše National Park, P. Homolka

IGC Proceedings (1993-2023)

This study describes the nutritional value of pastures in extreme mountain conditions in Krkonoše Mountains National Park. The Park performs important ecological and environmental functions. Extensive sheep grazing serves in the preservation of rare, protected and endangered species of plants (including endemics) and in the restoration of the biodiversity of meadows. The objective of this experiment was to estimate the production of plant biomass in these pastures and its digestibility by sheep.


Ruminal Proteolysis In Forages With Distinct Endopeptidases Activities, G. Pichard, C. Tapia, R. Larrain Feb 2023

Ruminal Proteolysis In Forages With Distinct Endopeptidases Activities, G. Pichard, C. Tapia, R. Larrain

IGC Proceedings (1993-2023)

Improving livestock efficiency in utilisation of nitrogen resources continues to be a major environmental and economic objective. Zhu et al. (1999) have shown that plant endopeptidases are activated as a response to cutting stress. Previous work in our laboratory explored over 300 entries of forage genotypes and found a broad diversity in enzymatic activity by means of hydrolysis in gelatine and direct autolysis assays in forage tissues. The objective of this work was to assess if the species previously identified as having high or low endopeptidase activity, would behave consistently when exposed to ruminal microbial proteolysis.


Effects Of Particle Size In Forage Samples For Protein Breakdown Studies, G. Pichard, C. Tapia Feb 2023

Effects Of Particle Size In Forage Samples For Protein Breakdown Studies, G. Pichard, C. Tapia

IGC Proceedings (1993-2023)

Coupling ruminal processes of hydrolysis and synthesis continues to be a research issue where more progress is needed. This requires the development of good protein assessment methods, particularly when representing the breakdown processes that occur in fresh pastures eaten by herbivores. Laboratory analyses need to deal with small and homogeneous samples, but the mechanical reduction of particle size may not reflect the actual digestion kinetics occurring when the original fresh forage is consumed. Such physical traits may alter the release of non-structural compounds and the penetration of microbial enzymes (Boudon et al., 2002). The objective of this work was …


Prediction Of Indigestible Ndf Content Of Grass And Legume Silages By Nirs, L. Nyholm, M. Rinne, M. Hellämäki, P. Huhtanen, J. Nousiainen Feb 2023

Prediction Of Indigestible Ndf Content Of Grass And Legume Silages By Nirs, L. Nyholm, M. Rinne, M. Hellämäki, P. Huhtanen, J. Nousiainen

IGC Proceedings (1993-2023)

The future feed evaluation systems based on mechanistic digestion models require reliable estimates of forage digestible and indigestible NDF content (DNDF and INDF). The objective of this study was to examine the potential of near infrared reflectance spectroscopy (NIRS) in predicting INDF content of grass and legume silages.


A New System For The Evaluation Of The Fermentation Quality Of Silages, K. Weiß, E. Kaiser Feb 2023

A New System For The Evaluation Of The Fermentation Quality Of Silages, K. Weiß, E. Kaiser

IGC Proceedings (1993-2023)

Depending on the content of nitrate in green forage, the pattern of fermentation products in silages differ significantly (Weiß & Kaiser, 2001). The systems, which are now common in practice for evaluating the quality of silage fermentation, characterise fermentation quality incorrectly because the evaluation is influenced by the chemical composition of green forage. The aim of this work was to derive an evaluation system for fermentation quality, which is independent from the chemical composition of green forage.


Analysis Of Silage Fermentation Characteristics Using Transflectance Measurements By Near Infrared Spectroscopy, A. Martínez, A. Soldado, R. Garcia, D. Sánchez, B. De La Roza-Delgado Feb 2023

Analysis Of Silage Fermentation Characteristics Using Transflectance Measurements By Near Infrared Spectroscopy, A. Martínez, A. Soldado, R. Garcia, D. Sánchez, B. De La Roza-Delgado

IGC Proceedings (1993-2023)

The fermentation end products as lactic acid, volatile fatty acids and ammonia-nitrogen, are important indicators of the efficiency of silage fermentation and are closely related to nutritive value of them (Jaster, 1995). Drying is problematic in the case of silage as many fermentation products are volatile and may get lost during the drying process. This may be a reason why NIR technology is being little used for the evaluation of silage fermentation characteristics. The feasibility of using near infrared transflectance spectroscopy to evaluate the content in fermentation end products of grass and maize fresh silage was investigated in this study.


Development Of A Method For The Fast And Complete Assessment Of Quality Characteristics In Undried Grass Silages By Means Of An Nir-Diode Array Spectrometer, H. Gibaud, C. Paul, J. M. Greef, B. Ruser Feb 2023

Development Of A Method For The Fast And Complete Assessment Of Quality Characteristics In Undried Grass Silages By Means Of An Nir-Diode Array Spectrometer, H. Gibaud, C. Paul, J. M. Greef, B. Ruser

IGC Proceedings (1993-2023)

Traditionally, the determination of grass silage is very time consuming and needs a lot of manpower and chemicals. The advantages of conventional laboratory NIRS instruments are well known but their disadvantage lies in their lacking suitability for on-farm use. A new type of spectrometer based on diode arrays may be used for this purpose. However, these new instruments still need to be calibrated for an accurate estimate of the fermentative and nutritive value of wet and unchopped grass silage.


A Simple Method For The Correction Of Fermentation Losses Measured In Laboratory Silos, F. Weissbach Feb 2023

A Simple Method For The Correction Of Fermentation Losses Measured In Laboratory Silos, F. Weissbach

IGC Proceedings (1993-2023)

Dry matter (DM) losses caused by formation of gaseous fermentation products can be measured by different methods. The most common method (A) is measuring the difference between the DM input and output of a silo. Other methods are based on the measurement of the fermentation gases which spontaneously leave the silo, either directly by collecting them (B) in a special absorbent like KOH or, much more easily, by weighing the filled silo at the beginning and the end of the fermentation process (C). The figures obtained by B and C are substantially smaller than those by A. This difference represents …


Prediction Of Red Clover Content In Mixed Swards By Near-Infrared Reflectance Spectroscopy, B. Deprez, D. Stilmant, C. Clément, C. Decamps, A. Peeters Feb 2023

Prediction Of Red Clover Content In Mixed Swards By Near-Infrared Reflectance Spectroscopy, B. Deprez, D. Stilmant, C. Clément, C. Decamps, A. Peeters

IGC Proceedings (1993-2023)

Because of the legume fixation capacity, their high protein content, digestibility and intake characteristics, more and more attention is paid to grassland clover content. In field experiments, clover content must often be determined, for example to quantify nitrogen flux or the best practices to manage such species (Stilmant et al., 2004). However hand sorting of clover and grass, even if accurate, is time-consuming and has a high labour cost. In comparison, accuracy of visual estimation of clover content, directly in the field, varies according to training and experience. Near-infrared reflectance spectroscopy (NIRS) has been proposed as a method for …


A Comparison Of Perennial Ryegrass Cultivars Differing In Heading Date And Grass Ploidy For Grazing Dairy Cows At Two Different Stocking Rates, M. O'Donovan, G. Hurley, L. Delaby, G. Stakelum Feb 2023

A Comparison Of Perennial Ryegrass Cultivars Differing In Heading Date And Grass Ploidy For Grazing Dairy Cows At Two Different Stocking Rates, M. O'Donovan, G. Hurley, L. Delaby, G. Stakelum

IGC Proceedings (1993-2023)

Animal productivity is the ultimate performance indicator of any new grass cultivar. Direct assessment is complex and expensive. Recent research has identified a number of important sward factors that influence intake and production. The most important factor appears to be green leaf mass. The objective of this two year study was to investigate the effects on milk yield, composition and grass intake of grass cultivars with contrasting heading dates (HD) and grass ploidies (PL) when grazed at different stocking rates.


Intake And Milk Production Of Lactating Dairy Cows Grazing Diverse Forage Mixtures Over Two Grazing Seasons, K. Soder, M. A. Sanderson, J. L. Stack, L. Muller Feb 2023

Intake And Milk Production Of Lactating Dairy Cows Grazing Diverse Forage Mixtures Over Two Grazing Seasons, K. Soder, M. A. Sanderson, J. L. Stack, L. Muller

IGC Proceedings (1993-2023)

Voluntary intake and stocking rate are key determinants of animal performance on pasture. Greater plant diversity in grassland plant communities has been linked to increased primary production, greater stability in response to disturbance, and reduced weed pressure. Thus, increasing plant diversity may be one approach to improving animal productivity. An experiment was conducted to determine the effects of forage diversity on intake and milk production of lactating dairy cows over two grazing seasons.


In Situ Rumen Degradability Of Perennial Ryegrass Cultivars Differing In Ploidy And Heading Date In Ireland, V. Olsson, J. J. Murphy, F. P. O'Mara, M. O'Donovan, F. J. Mulligan Feb 2023

In Situ Rumen Degradability Of Perennial Ryegrass Cultivars Differing In Ploidy And Heading Date In Ireland, V. Olsson, J. J. Murphy, F. P. O'Mara, M. O'Donovan, F. J. Mulligan

IGC Proceedings (1993-2023)

Grazed grass is the predominant feed in Irish dairy and beef cattle production systems. Knowledge of the degradability characteristics of protein in Irish forages is necessary for the establishment of protein values (PDIE and PDIN values) for these. This knowledge would also facilitate more accurate formulation of supplements for grass diets with the potential to reduce nitrogen (N) excretion.


Grazing Behaviour Of Beef Steers Grazing Kentucky 31 Endophyte Infected Tall Fescue, Q4508-Ar542 Novel Endophyte Tall Fescue, And Lakota Prairie Grass, H. T. Boland, G. Scaglia, J. P. Fontenot, A. O. Abaye, R. Smith Feb 2023

Grazing Behaviour Of Beef Steers Grazing Kentucky 31 Endophyte Infected Tall Fescue, Q4508-Ar542 Novel Endophyte Tall Fescue, And Lakota Prairie Grass, H. T. Boland, G. Scaglia, J. P. Fontenot, A. O. Abaye, R. Smith

IGC Proceedings (1993-2023)

Tall fescue is the most dominant grass used for pasture in the U.S. covering over 14 million ha. As a result, fescue toxicosis is a major concern among producers, especially during the summer months when the symptoms, such as reduced weight gains, are most pronounced. Producers need alternative forages for grazing cattle that do not have the negative effects associated with endophyte infected tall fescue. The objective of this experiment was to determine the grazing behaviour of cattle on Kentucky 31 endophyte infected (E+) tall fescue (Festuca arundinacea Schreb.), Q4508-AR542 (Q) novel endophyte tall fescue, and Lakota (L) …


Caucasian Clover Is More Productive Than White Clover In Temperate Pastures, A. D. Black, D. J. Moot, R. J. Lucas Feb 2023

Caucasian Clover Is More Productive Than White Clover In Temperate Pastures, A. D. Black, D. J. Moot, R. J. Lucas

IGC Proceedings (1993-2023)

White clover (wc) (Trifolium repens) is present and is often the dominant legume in the >11 m ha of grassland in New Zealand (NZ). However, wc has limitations and normally contributes less than 20% of total annual pasture dry matter (DM) production. The use of a wider range of legume species is one way to increase legume percentage in wc/grass pastures. Caucasian (Cc) or Kura clover (Trifolium ambiguum) is a persistent legume which is slower to establish than wc but can increase total legume production (Cc plus volunteer wc) and hence N2 fixation and animal productivity. …


Response Of Warm-Season Grass Pasture To Grazing Period And Recovery Period Lengths, B. E. Anderson, W. H. Schacht Feb 2023

Response Of Warm-Season Grass Pasture To Grazing Period And Recovery Period Lengths, B. E. Anderson, W. H. Schacht

IGC Proceedings (1993-2023)

Grazing period and recovery period lengths are key variables influencing grassland production and composition. Systems with short grazing periods and lengthy recovery periods require numerous pastures. Relatively high facility and management costs associated with multiple-pasture systems can be justified only if plant response is favorable and/or if livestock production is improved. This study determined the effects of 4 different combinations of grazing period/recovery period lengths on percentage basal cover (PBC) and relative species composition (RSC) of seeded, warm-season grass pasture.


Yield Components In A Signal Grass-Clitoria Mixture Grazed At Different Herbage Allowance, R. Jiménez-Guillen, S. Rojas-Hernández, J. Olivares-Pérez, A. Martínez-Hernández, J. Pérez-Pérez Feb 2023

Yield Components In A Signal Grass-Clitoria Mixture Grazed At Different Herbage Allowance, R. Jiménez-Guillen, S. Rojas-Hernández, J. Olivares-Pérez, A. Martínez-Hernández, J. Pérez-Pérez

IGC Proceedings (1993-2023)

A Signal grass-Clitoria mixture provides good quality forage in the dry tropic of southern Mexico. However, its response in leaf and stem yields to grazing at different daily herbage allowances is not well documented. The objective of this study was to determine available and residual leaf and stem yields in a Signal grass (Brachiaria decumbens)-Clitoria (Clitoria ternatea) mixture grazed at different daily herbage allowance.


Renovation-Year Forage Quality Of Grass Pastures Sod-Drilled With Kura Clover, P. R. Peterson, P. Seguin, G. Laberge, C. C. Sheaffer Feb 2023

Renovation-Year Forage Quality Of Grass Pastures Sod-Drilled With Kura Clover, P. R. Peterson, P. Seguin, G. Laberge, C. C. Sheaffer

IGC Proceedings (1993-2023)

Including legumes can enhance yield, quality, and animal performance potential of grass pastures. Kura clover is an exceptionally winter hardy forage legume with high forage quality (Taylor & Smith, 1998). However, its seedling vigor is poor. Herbicide sod suppression prior to sod drilling enabled kura clover to establish in the north central USA and eastern Canada (Cuomo et al., 2001; Laberge et al., 2005), but its percentage of renovation-year forage yield was less than for sod-seeded red or white clover. The objective of this study was to determine the influence of herbicide suppression and clover species on renovation-year …


Farm Performance From Holstein-Friesian Cows Of Three Genetic Strains On Grazed Pasture, K. A. Macdonald, B. S. Thorrold, C. B. Glassey, J. A. S. Lancaster, G. A. Verkerk, J. E. Pryce, C. W. Holmes Feb 2023

Farm Performance From Holstein-Friesian Cows Of Three Genetic Strains On Grazed Pasture, K. A. Macdonald, B. S. Thorrold, C. B. Glassey, J. A. S. Lancaster, G. A. Verkerk, J. E. Pryce, C. W. Holmes

IGC Proceedings (1993-2023)

Dairy selection objectives and farm production systems in USA and Europe are different from those in New Zealand (NZ). The use of overseas semen in NZ in the last 20 years has changed the genetics of the former NZ Holstein-Friesian (HF) strain. This trial was designed to demonstrate the genetic progress in the NZ HF dairy herd in the last 25 years and how high production potential North American HF cows perform under pasture-based feeding systems.


Forage Yield And Quality Of Signal Grass-Clitoria Mixture Grazed At Different Frequencies, R. Jiménez-Guillen, J. Olivares-Pérez, S. Rojas-Hernández, A. Martínez-Hernández Feb 2023

Forage Yield And Quality Of Signal Grass-Clitoria Mixture Grazed At Different Frequencies, R. Jiménez-Guillen, J. Olivares-Pérez, S. Rojas-Hernández, A. Martínez-Hernández

IGC Proceedings (1993-2023)

A Signal grass-Clitoria mixture provides good quality forage in the dry tropic of southern Mexico. Grazing frequency is a management tool that determines yield, botanical components and quality of pastures. The objective of this study was to determine forage yield, quality and botanical components in a Signal grass (Brachiaria decumbens)-Clitoria (Clitoria ternatea) mixture when grazed at different frequencies.


Genetic Alternatives For Dairy Producers Who Practise Grazing, H. D. Norman, J. R. Wright, R. L. Powell Feb 2023

Genetic Alternatives For Dairy Producers Who Practise Grazing, H. D. Norman, J. R. Wright, R. L. Powell

IGC Proceedings (1993-2023)

The decline in cow fertility has had a negative impact on all dairy producers, especially those that practise seasonal calving with pasture-based dairying. One alternative that is being tried in the United States (US) by a few graziers is to use bulls from New Zealand (NZ) because NZ producers have practised seasonal calving for some time. However, genotype-environment interaction is a concern; genetic correlations that were derived by the International Bull Evaluation Service (2004) between bull rankings from different countries were often lower for NZ than for other countries. The objective of this study was to compare the performance of …


Suitability Of Small And Large Size Dairy Cows In A Pasture-Based Production System, M. Steiger Burgos, R. Petermann, P. Hofstetter, P. Thomet, S. Kohler, A. Munger, J. W. Blum, P. Kunz Feb 2023

Suitability Of Small And Large Size Dairy Cows In A Pasture-Based Production System, M. Steiger Burgos, R. Petermann, P. Hofstetter, P. Thomet, S. Kohler, A. Munger, J. W. Blum, P. Kunz

IGC Proceedings (1993-2023)

Pasture-based dairy production with greatly reduced supplemental feeding and block-calving in spring is increasingly applied in Switzerland. The prevalent cow type has been selected mainly for high individual production in a barn feeding system with balanced diet. This cow type has continuously increased in size over the last 30 years. The question arises whether this type is suitable for the new system, and particularly if cow size is a critical factor. Theoretically a large, heavy type of cow has a higher intake capacity, while the nutrient requirements for a small, light type are easier to satisfy.


Effect Of Stocking Rate And Grazing System On Fine And Superfine Merino Wool Production And Quality On Native Swards Of Uruguay, I. De Barbieri, F. Montossi, E. J. Berretta, A. Dighiero, A. Mederos Feb 2023

Effect Of Stocking Rate And Grazing System On Fine And Superfine Merino Wool Production And Quality On Native Swards Of Uruguay, I. De Barbieri, F. Montossi, E. J. Berretta, A. Dighiero, A. Mederos

IGC Proceedings (1993-2023)

Modern textile tendencies show that consumers prefer light, soft, resistant, natural, and comfortable clothes, for which fine and superfine wools are in great demand, particularly at the high value markets (Whiteley, 2003). The main objective of the present study was to define sustainable stocking rates and grazing systems on native swards for fine and superfine wool production in the Basaltic region of Uruguay.


The Effect Of Stocking Rate And Lamb Grazing System On Sward Performance Of Trifolium Repens And Lotus Corniculatus In Uruguay, F. Montossi, R. San Julián, M. Nolla, M. Camesasca, F. Preve Feb 2023

The Effect Of Stocking Rate And Lamb Grazing System On Sward Performance Of Trifolium Repens And Lotus Corniculatus In Uruguay, F. Montossi, R. San Julián, M. Nolla, M. Camesasca, F. Preve

IGC Proceedings (1993-2023)

Lambs have a great potential to diversify and stimulate meat and wool production and economical returns within the industry. The main objective of this study was to evaluate different feeding and management alternatives for the production of high quality wool and meat as well as their effects on sward characteristics in the Basaltic region of Uruguay.


Cattle And Sheep Mixed Grazing 1: Species Equivalence, R. D. Améndola-Massiotti, S. J. C. González-Montagna, P. A. Martínez-Hernández Feb 2023

Cattle And Sheep Mixed Grazing 1: Species Equivalence, R. D. Améndola-Massiotti, S. J. C. González-Montagna, P. A. Martínez-Hernández

IGC Proceedings (1993-2023)

The effects of mixed grazing of cattle and sheep depend on stocking rate (SR) and species ratio (Nicol, 1997). Calculations of SR and species ratio require the use of species equivalence. Equivalents are often estimated in terms of intake requirements calculated on the basis of LW0.75. Freer (1981) stated that LW0.9 would be more appropriate for comparisons of intake requirements for maintenance of sheep and cattle. Nonetheless, Nolan & Connolly (1977) stated that the equivalence is system-specific and depends on the species being considered. The objective of this experiment was to estimate species equivalence for the evaluation …