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Full-Text Articles in Physical Sciences and Mathematics

Introduction Of Vegetative And Reproductive Characters Into Trifolium Repens By Interspecific Hybridisation, A H. Marshall, T P.T Michaelson-Yeates, M Meredith, D H. Hides Mar 2024

Introduction Of Vegetative And Reproductive Characters Into Trifolium Repens By Interspecific Hybridisation, A H. Marshall, T P.T Michaelson-Yeates, M Meredith, D H. Hides

IGC Proceedings (1993-2023)

Interspecific hybridization between white clover (Trifolium repens L.) and ball clover (T.nigrescens) and Caucasian clover (T.ambiguum) is a means of improving the reproductive growth and persistency of T.repens. F1 hybrids between T.repens and T.nigrescens were produced without ovule culture and confirmed as true hybrids by chromosome counts, isoenzyme analysis and leaf markers. Their growth habit was generally intermediate between the two parents. After backcrossing to T.repens the BC1 and BC2 hybrids had more of the characteristics associated with T.repens though they retained some of the greater reproductive growth of …


Colchicine-Doubling Of Germinating Seedlings Of Interspecific Wildrye Hybrids, T A. Jones, D C. Nielson, H Jaussi Mar 2024

Colchicine-Doubling Of Germinating Seedlings Of Interspecific Wildrye Hybrids, T A. Jones, D C. Nielson, H Jaussi

IGC Proceedings (1993-2023)

Colchicine has usually been applied to sterile clones of interspecific grass hybrids to restore fertility. However, when hybrids are partially fertile, colchicine can be applied to germinating seedlings. Four populations of Basin wildrye (Leymus cinereus) X beardless wildrye (L. triticoides) were treated with a 0.2% colchicine solution to double chromosome number from 4x=28 to 8x=56. Doubling percentage was 28, 33, 42, and 44% of all root-tips examined for the four populations. When plants without doubled sectors were discarded, doubling percentage increased to 56, 68, 78, and 78%. Plants with doubled sectors have been placed in crossing blocks …


Germplasm Enhancement Of Annual Forage Legumes Suitable For Use In Cold Highland Environments, H K. Firincioglu, N Karagullu, S Unal, A M. Abd-El Moneim Feb 2024

Germplasm Enhancement Of Annual Forage Legumes Suitable For Use In Cold Highland Environments, H K. Firincioglu, N Karagullu, S Unal, A M. Abd-El Moneim

IGC Proceedings (1993-2023)

The Central Highlands (CH) of Turkey are characterized by cold winters and dry summers. Under these extreme air temperatures, forage crops are still underexploited as a part of crop rotations. Half of the total vetch hectarage in Turkey is in the CH, and farmers still use the local cultivars that have good adaptability but low yield potential. Therefore, with the aim of identifying and improving the annual forage legume species for the cold and dry environment of the CH, research work was carried out during the 1993/94 and 1994/ 95 crop seasons. Initial results showed that Hungarian vetch (Vicia …


Use Of The Grazing Animal In Forage Breeding, J H. Bouton, C S. Hoveland, R N. Gates Feb 2024

Use Of The Grazing Animal In Forage Breeding, J H. Bouton, C S. Hoveland, R N. Gates

IGC Proceedings (1993-2023)

The objective of these studies was to determine, in the context of a forage breeding program, the effect of cattle grazing on survival of alfalfa (Medicago sativa L.), red clover (Trifolium pratense L.), and tall fescue (Festuca arundinacea Schreb.). Different cultivars of each species were compared in grazed and ungrazed areas. Results consistently demonstrated that grazing reduced plant stands when compared to ungrazed conditions for each species tested. In alfalfa, however, cultivars developed for grazing tolerance showed much better grazing survival. Infection with the fungal endophyte, Acremonium coenophialum Morgan-Jones & Gams, likewise substantially increased grazing survival in …


Study Of Genotype X Environment Interaction In Alfalfa Forage Yield, S N. Acharya, G C. Kozub, H Najda, A Aasen Feb 2024

Study Of Genotype X Environment Interaction In Alfalfa Forage Yield, S N. Acharya, G C. Kozub, H Najda, A Aasen

IGC Proceedings (1993-2023)

The response of alfalfa (Medicago sativa L.) forage yield to eight Alberta test sites was studied for the 1990 and 1991 production years. Cluster analysis was used to group locations and cultivars. Analyses of variances indicated genotype x environment (location) interaction for the first cut yield, the total yield and the difference between first and second cut yields. The Brooks, Bow Island (irrigation), Bow Island (dryland) and Provost locations always clustered together indicating that three of these four test sites may be eliminated without sacrificing reliability.


Selection For Self-Fertility And Self-Fertility In Alfalfa As A Tool For Breeding Strategy Assessment, D Rosellini, F Veronesi, F Lorenzetti Feb 2024

Selection For Self-Fertility And Self-Fertility In Alfalfa As A Tool For Breeding Strategy Assessment, D Rosellini, F Veronesi, F Lorenzetti

IGC Proceedings (1993-2023)

Cultivar development in alfalfa relies on heterosis, that has been mainly exploited by intercrossing selected clones from diverse gemplasm sources; an alternative way was proposed, based on developing double cross cultivars obtained by crossing clones selected for self-sterility, propagated by somatic seed. A different breeding strategy was recently emphasized based on population improvement through inbreeding and recurrent selection; in this case, self-sterility would be selected against. If self-sterility is determined by genetic load, the second strategy should be preferred, and selecting for self-fertility may bring about positive correlated responses for forage yield. Divergent selection for self-fertility was applied in a …


Morphological And Rapd Variations And Regenerants Derived From Cell Suspension Culture Of Pangolagrass, C S. Chen, S M. Wang, Y K. Cheng Feb 2024

Morphological And Rapd Variations And Regenerants Derived From Cell Suspension Culture Of Pangolagrass, C S. Chen, S M. Wang, Y K. Cheng

IGC Proceedings (1993-2023)

Pangolagrass cv. A254, a sterile strain, is one of Taiwan's major forage sources but recently has faced serious disease problems. Tissue culture was expected to generate variations in the breeding program of pangolagrass. The purpose of this study is to examine the somaclonal variation of regenerated plants by means of phenotypic characters survey and RAPD analysis. After long-term suspension culture and EMS mutagenesis, many regenerated plants with morphological variations have been found. Among the regenerants, 10 clones with obviously different characteristics were selected for further studies. Nine of them showed significant differences in morphological trains as compared to A254. By …


Phylogenetics Of Apomictic Common Dallisgrass (Paspalum Dilatatum), B L. Burson, M A. Hussey Feb 2024

Phylogenetics Of Apomictic Common Dallisgrass (Paspalum Dilatatum), B L. Burson, M A. Hussey

IGC Proceedings (1993-2023)

Common dallisgrass, Paspalum dilatatum Poir., an important warm season forage grass, is an obligate apomict with 50 chromosomes which associate as 20 bivalents and 10 univalents during meiosis. Because efforts to improve the grass have not been successful, a phylogenetic investigation was initiated to identify the progenitors of common dallisgrass in an effort to circumvent the apomictic barrier to improvement. The genomic composition has been determined for four dallisgrass biotypes: yellow-anthered (2n=4x=40) IIJJ; common (2n=5x=50) IIJJX; Uruguayan (2n=6x=60) IIJJXX; and Uruguaiana (2n=6x=60) IIJJXX. While the source of the X genome is unknown, the genes controlling apomixis are on at least …


Increased Longevity Of Red Clover Through Selection For Persistence And Disease Resistance, R R. Smith Feb 2024

Increased Longevity Of Red Clover Through Selection For Persistence And Disease Resistance, R R. Smith

IGC Proceedings (1993-2023)

Red clover (Trifolium pratense L.), an excellent perennial forage legume, has been considered for many years to be a short-lived, two to three years, forage. Selection for resistance to foliar and root rot diseases and for longevity of stand over the past four decades has improved the persistence of the currently available cultivars. The present studies were conducted to evaluate the progress from selection for longevity and disease resistance. Selection for stand longevity and improved disease resistance has reliably extended the production of red clover into the third and fourth year. Selection for healthy plants in 3- or …


Backcrosses Of Diploid Lolium-Festuca Hybrids To Festuca, W Nitzche Feb 2024

Backcrosses Of Diploid Lolium-Festuca Hybrids To Festuca, W Nitzche

IGC Proceedings (1993-2023)

Fertile diploid hybrids of Lolium x Festuca -backcrosses were produced by colchicine-treatment without chromosome doubling. The hybrids have new combinations of their characters, not known in the natural species.


Selection For Herbage Yield And Flowering In Ladino White Clover, J A. Garcia Feb 2024

Selection For Herbage Yield And Flowering In Ladino White Clover, J A. Garcia

IGC Proceedings (1993-2023)

A geno-phenotypic recurrent selection program is being carried out on ladino white clover (Trifolium repens L.) aiming to combine good DM and seed yields. Selection criteria includes plant density, vigor, winter growth, flowering and diseases resistance. The performance of selections in terms of DM yields and seed production was evaluated in separate trials. Two cycles of selection increased flowering by 36% and seed yield by 59%. Preliminary data on herbage yields shows a very good performance of the selected material and suggests the feasibility of combining high DM and seed yields.


Selection For Field Survival Increases Freezing Tolerance In Festulolium, M D. Casler, P G. Pitts, P C. Bilkey, C A. Rose-Fricker Feb 2024

Selection For Field Survival Increases Freezing Tolerance In Festulolium, M D. Casler, P G. Pitts, P C. Bilkey, C A. Rose-Fricker

IGC Proceedings (1993-2023)

Festulolium (Festulolium braunii K.A.) is marginally adapted to the north central and northeastern USA and southern Canada. The purpose of this study was to evaluate four festulolium populations selected for field survival under harsh winter conditions for their freezing tolerance in controlled environments. Progenies of all four populations showed some improvement in freezing tolerance compared to their parents. Improvements were dependent on the temperature at which measurements were made and varied among germplasms. Improvements were manifested in both decreased plant mortality and decreased injury to surviving plants. Genetic variation for freezing tolerance appears to be a viable mechanism …


Fitness Of Apomictic And Sexual Buffelgrass Germplasm, M A. Hussey, Yue-Wen Wang, B L. Burson Feb 2024

Fitness Of Apomictic And Sexual Buffelgrass Germplasm, M A. Hussey, Yue-Wen Wang, B L. Burson

IGC Proceedings (1993-2023)

Breeding apomictic grasses is based on either the direct selection of apomictic landraces or the hybridization of sexual and apomictic types and selection of apomictic F1 hybrids. Little is known about the potential of sexual hybrids as cultivars in apomictic species. To determine if method of reproduction (MOR) was related to plant vigor, parental, S1, and F1 hybrids of buffelgrass differing in method of reproduction (apomictic or sexual) were established in a common garden in 1994 and 1995. In both years severe inbreeding depression was observed in the S1 progeny as they were shorter, produced less biomass, and had low …


Selection For Nitrogen Use Efficiency In Perennial Ryegrass Using Hydroponics, E N. Van Loo, A.J. P. Van Wijk, O Dolstra, H.J. P. Marvin, C.H. A. Snijders Feb 2024

Selection For Nitrogen Use Efficiency In Perennial Ryegrass Using Hydroponics, E N. Van Loo, A.J. P. Van Wijk, O Dolstra, H.J. P. Marvin, C.H. A. Snijders

IGC Proceedings (1993-2023)

A selection method was tested for improving nitrogen use efficiency of perennial ryegrass (Lolium perenne L.) using a hydroponics system that controls the plant nitrogen concentration. Divergent selection for plant production, leaf area increase and dry matter distribution in six segregating populations under limiting nitrogen supply resulted in differences between offspring of upward and downward selections larger than 20 % in most characteristics. Moderately high realized heritabilities were found for some characteristics (up to 0.88). It is concluded that early selection for nitrogen use efficiency is feasible using this hydroponics system.


Development Of A Multicross Cultivars Of Stylosanthes Spp., B Grof, C D. Fernandes, C B. Almeida, A V. Dos Santos Feb 2024

Development Of A Multicross Cultivars Of Stylosanthes Spp., B Grof, C D. Fernandes, C B. Almeida, A V. Dos Santos

IGC Proceedings (1993-2023)

A synthetic population, referred to as multicross, has been developed from an interspecific mixture of Stylosanthes capitata and S.macrocephala germplasm. This basic population evolved in a farming situation subject to uncontrolled, continuous, heavy grazing. Natural selection and systematically programmed intercrossing among anthracnose-resistant Venezuelan and Brazilian genotypes of S.capitata have played major roles in the subsequent development of this heterogeneous population. Eight anthracnose-resistant Venezuelan and two Brazilian accessions of S.capitata were introgressed into the basic population. Genetic diversity was further increased by adding seed of four selected accessions of S. macrocephala to the synthetic which had already included …


Selection Of Stylosanthes Guianensis For The Cerrados Of Brazil, B Grof, C D. Fernandes, A V. Dos Santos, C B. Almeida Feb 2024

Selection Of Stylosanthes Guianensis For The Cerrados Of Brazil, B Grof, C D. Fernandes, A V. Dos Santos, C B. Almeida

IGC Proceedings (1993-2023)

Progenies of intervarietal hybrid stylos (Stylosanthes guianensis var.vulgaris x S.guianensis var.pauciflora) with mid-season maturity date (early-and mid-June), high dry matter and seed yields have been identified at the National Beef Cattle Research Center (CNPGC), Campo Grande, MS, Brazil. Dry matter yields of the best selections were comparable to those of the late-flowering control, cv. Mineirão. Several mid-season hybrids produced 150 to 200 kg/ha of seed-inpod and a single plant selection yielded in excess of 300 kg/ha. The high degree of resistance to anthracnose observed in the advanced generations is attributed to the immense genetic diversity of these …


Development Of Red Clover With High Levels Of Resistance To Root-Knot Nematodes, K H. Quesenberry, R A. Dunn, D E. Moon Feb 2024

Development Of Red Clover With High Levels Of Resistance To Root-Knot Nematodes, K H. Quesenberry, R A. Dunn, D E. Moon

IGC Proceedings (1993-2023)

Red clover (Trifolium pratense L.), is grown in many areas of the world, but production in subtropical regions may be limited by susceptibility to root-knot nematodes (Meloidogyne spp.) (RKN). Selection for early vigor in RKN infested soils resulted in moderate RKN tolerance in ‘Cherokee’. However, improved RKN resistance in red clover was needed. Seven additional cycles of greenhouse selection using Cherokee as a base population developed a population (FLMR7) with high RKN resistance. When FLMR7 was infested with M. arenaria, M. hapla, M. incognita, or M. javanica, numbers of galls and egg masses …


Evaluating White Clover For Resistance To Cylindrocladium Root Rot, D S. Wofford, A Amaya Feb 2024

Evaluating White Clover For Resistance To Cylindrocladium Root Rot, D S. Wofford, A Amaya

IGC Proceedings (1993-2023)

A rapid screening technique was developed for use in evaluating white clover (Trifolium repens L.) for resistance to Cylindrocladium root rot incited by Cylindrocladium crotalariae. Culture plates of the fungus were evaluated for number of infective propagules after four weeks of growth on potato dextrose agar, and no differences were detected among plates. Seedlings of ‘Osceola’ white clover were exposed in the greenhouse to four levels of inoculum over a six-week period, with resistance based on percent survival of uninoculated controls. It was shown that suspending 1 culture plate in 100ml of water and rating plants four weeks …


Characterization Of Chilling Sensitivity Of Tropical And Temperate Grasses, M Ebina, K Okumura, H Matsu-Ura, Y Tsurumi Feb 2024

Characterization Of Chilling Sensitivity Of Tropical And Temperate Grasses, M Ebina, K Okumura, H Matsu-Ura, Y Tsurumi

IGC Proceedings (1993-2023)

C16-C18 fatty acid composition of some lipids in several species of temperate and tropical grasses were measured. The fatty acid composition of phosphatidylglycerol (PG) and sulfoquinovosyl diglyceride (SQDG) indicated significant differences between temperate and tropical grasses. Especially the differences of the fatty acid composition of PG were remarkable. The unsaturated fatty acid content of PG in tropical grasses was lower than the contents in temperate grasses. These differences constantly appeared through the year. These differences were caused by the low content of polyunsaturated fatty acids, or the low contents of C18 fatty acids.


Characterization Of Somatic Hybrids Between Festuca Arundinacea And Lolium Multiflorum, M Fujimori, T Takamizo, K Suginobu Feb 2024

Characterization Of Somatic Hybrids Between Festuca Arundinacea And Lolium Multiflorum, M Fujimori, T Takamizo, K Suginobu

IGC Proceedings (1993-2023)

Metabolically inactivated tall fescue (Festuca arundinacea Schreb.) protoplasts and X-ray irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts were electrofused resulting in the regeneration of twenty-six plantlets. Southern blot analysis using three mitochondrial probes and random amplified polymorphic DNA (RAPD) analysis revealed that the regenerants were intergeneric somatic hybrids. All regenerated plants were male sterile and some of them had stamens that exhibited the pistillode appearance.


Selecting Lucerne (Medicago Sativa Complex) For Traits Conferring Adaptation To Grazing, E Piano, L Pecetti, M Romani Feb 2024

Selecting Lucerne (Medicago Sativa Complex) For Traits Conferring Adaptation To Grazing, E Piano, L Pecetti, M Romani

IGC Proceedings (1993-2023)

A germplasm collection of the Medicago sativa complex was evaluated to select genotypes possessing traits possibly conferring adaptation to grazing. Deep-crowned plants, either creeping-rooted or rhizomatous, were found with different frequencies, in all “taxonomic” groups examined. Although belonging to subsp. sativa, “mielga” wild populations from Spain were closer to subsp. falcata for morpho-physiological traits. Deep-crowned plants could be categorised into four top-growth models, to which corresponded a fairly distinct underground morphology: three were rhizomatous and one creeping-rooted. Cultivars selected for creeping-rootedness did not outyield rhizomatous populations of subsp. x varia which, in turn, had better spreading ability. In a …


Selection For Seed Size And Coleoptile Length In Timothy (Phleum Pratense L.), Hans Arne Jonsson Feb 2024

Selection For Seed Size And Coleoptile Length In Timothy (Phleum Pratense L.), Hans Arne Jonsson

IGC Proceedings (1993-2023)

The intention of this study was to show the effect of selection for seed weight and coleoptile length on morphology and agronomically important characters in timothy (Phleum pratense L.). Two cycles of selection increased the seed weight as well as the length of coleoptile and root. The emergence from deep sowing in sand and in the field were insignificantly increased, whereas the percentage stand and the dry matter yield were decreased, albeit insignificantly. Inbreeding and linkage effects were considered possible causes for this.


Differential Energy Allocation Among 15 New Guineagrass (Panicum Maximum Jacq.) Hybrids, J A. Usberti Jr, R Usberti, R S. Paterniani Feb 2024

Differential Energy Allocation Among 15 New Guineagrass (Panicum Maximum Jacq.) Hybrids, J A. Usberti Jr, R Usberti, R S. Paterniani

IGC Proceedings (1993-2023)

The main aim of this research work was to determine trends of energy allocation among newly developed guineagrass (Panicum maximum Jacq) hybrids, ranging from very-early to late-flowering genotypes. Besides the flowering cycle, eight phenological and two seed quality traits were scored in a greenhouse randomized complete block experiment including plant height (PH), reproductive tiller number/overall tiller number (RTN/OTN), panicle number/reproductive tiller (PN/RT), leaf length (LL), leaf width (LW), panicle length (PL), fresh weight (FW), dry weight (DW), number of seeds/gram (NS/G) and seed sample physical purity (SPP). Very-early and early-flowering hybrids consistently showed the highest correlations values between flowering …


Improvement Of Forage Quality By Means Of Biotechnology: Stable Transformation Of Warm-Season Grasses By Particle Bombardment, C Yuge, R Akashi, O Kawamura Feb 2024

Improvement Of Forage Quality By Means Of Biotechnology: Stable Transformation Of Warm-Season Grasses By Particle Bombardment, C Yuge, R Akashi, O Kawamura

IGC Proceedings (1993-2023)

We have used a simple and inexpensive, self-built particle acceleration apparatus for direct delivery of DNA to cultured cells of warm-season grasses. High levels of transient expression of the b-glucuronidase gene were obtained following bombardment of embryogenic suspension cells and calli of bahiagrass (Paspalum notatum Flügge) and dallisgrass (Paspalum dilatatum Poir). Furthermore, stable transformed calli of both species have been obtained using this simple particle gun.


Transformation In Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense Rna, R Akashi, T Uchiyama, Y Sakatani, O Kawamura Feb 2024

Transformation In Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense Rna, R Akashi, T Uchiyama, Y Sakatani, O Kawamura

IGC Proceedings (1993-2023)

We have developed a rapid and reproducible transformation system for bird’s-foot trefoil (Lotus corniculatus L.) by using Agrobacterium-mediated T-DNA transfer and the incorporation of the antisense gene for cinnamyl alcohol dehydrogenase (CAD) from Aralia cordata into Lotus for lignin reduction. The presence of the transferred antisense gene in regenerated plants has been confirmed by PCR analysis.


Possibility Of Selection For Mineral Concentration In Orchardgrass By X-Ray Microanalysis, S Saiga, Y Nishimura, K Izumi Feb 2024

Possibility Of Selection For Mineral Concentration In Orchardgrass By X-Ray Microanalysis, S Saiga, Y Nishimura, K Izumi

IGC Proceedings (1993-2023)

To assess the ability of X-ray microanalysis for screening breeding populations of orchardgrass in mineral concentration, correlations between chemical and X-ray analysis were investigated. Six orchardgrass plants with high- and low-Mg concentrations were examined for three harvest dates in 1995. The samples were separated into leaf blade, leaf sheath, culm and head. Mg concentration in leaf and head were higher than in sheath and in culm. P concentration was highest in the head, and lowest in the sheath. Correlations between chemical and X-ray analysis were r=0.692***, 0.526***, 0.252* and 0.562*** for Mg, Ca, K and P, respectively. Correlation coefficients between …


Aluminum Tolerance Qtl In Diploid Alfalfa, M K. Sledge, J H. Bouton, J Tamulonis, W A. Parrot, G Kochert Feb 2024

Aluminum Tolerance Qtl In Diploid Alfalfa, M K. Sledge, J H. Bouton, J Tamulonis, W A. Parrot, G Kochert

IGC Proceedings (1993-2023)

Aluminum (Al) toxicity associated with acid soils greatly inhibits alfalfa (Medicago sativa L.) productivity throughout much of the world’s major grassland areas. In this paper, we report the identification of quantitative trait loci (QTL) controlling aluminum tolerance in diploid alfalfa (Medicago sativa L). An in vitro callus growth bioassay was used to select aluminum tolerant and aluminum sensitive parents, and to screen an F2 population for aluminum tolerance. Fifty-eight cDNA probes were mapped to nine linkage groups, and the F2 genotypic classes were contrasted with means from the callus growth bioassay using ANOVA. We also used Mapmaker-QTL to …


Selecting Acid-Soil Tolerant White Clover, P W. Voigt, D R. Morris Feb 2024

Selecting Acid-Soil Tolerant White Clover, P W. Voigt, D R. Morris

IGC Proceedings (1993-2023)

A two-stage, tandem, recurrent-selection program is proposed to improve acid-soil tolerance of white clover, Trifolium repens L. In stage one, we evaluate root growth of very young seedlings, grown in an highly-acid Al toxic soil. Although the relationship between the acid-soil response of very young seedlings and that of mature white clover is unknown, the failure of white clover to become established on a highly-acid, Al-toxic soil suggests that establishment is a critical stage of plant development on such soils. In stage two we reevaluate genotypes selected in stage one, in a small-pot study at two lime levels, to separate …


Lodging Resistance Evaluation In Lolium Multiflorum, Katsuo Egara, Kazuhiro Tase, Makoto Kobayashi, Hiroki Fujii Feb 2024

Lodging Resistance Evaluation In Lolium Multiflorum, Katsuo Egara, Kazuhiro Tase, Makoto Kobayashi, Hiroki Fujii

IGC Proceedings (1993-2023)

The objective of this study was to develop a simple technique to evaluate lodging resistance in Italian ryegrass (Lolium multiflorum) for genetic improvement. Significant relationships were recognized between the pushing resistance index and actual lodging degree (ALD), and between the breaking resistance index and ALD, at the 0.1 and 5 percent levels, respectively.


Mechanistic Modelling Of Grassland Energy Balance, G D. Buchan, B Main, B A. Mckenzie Feb 2024

Mechanistic Modelling Of Grassland Energy Balance, G D. Buchan, B Main, B A. Mckenzie

IGC Proceedings (1993-2023)

No abstract provided.