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Full-Text Articles in Physical Sciences and Mathematics

6 Tips Mudah Pengobatan Daging Tumbuh Di Penis Paling Aman, Kutil Kelamin Ampuh Jun 2019

6 Tips Mudah Pengobatan Daging Tumbuh Di Penis Paling Aman, Kutil Kelamin Ampuh

kutil kelamin Ampuh

6 Tips Mudah Pengobatan Daging Tumbuh Di Penis Paling Aman-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belum mengetahui tentang tumbuh daging dikemaluan.Daging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil biasanya …


5 Cara Mudah Merontokan Daging Tumbuh Di Miss V Paling Manjur, Kutil Kelamin Ampuh Jun 2019

5 Cara Mudah Merontokan Daging Tumbuh Di Miss V Paling Manjur, Kutil Kelamin Ampuh

kutil kelamin Ampuh

5 Cara Mudah Merontokan Daging Tumbuh Di Miss V Paling Manjur-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belum mengetahui tentang tumbuh daging dikemaluan.Daging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil …


5 Cara Mudah Menyembuhkan Daging Tumbuh Di Miss V Terbukti Manjur, Kutil Kelamin Ampuh Jun 2019

5 Cara Mudah Menyembuhkan Daging Tumbuh Di Miss V Terbukti Manjur, Kutil Kelamin Ampuh

kutil kelamin Ampuh

5 Cara Mudah Menyembuhkan Daging Tumbuh Di Miss V Terbukti Manjur-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belum mengetahui tentang tumbuh daging dikemaluan.Daging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil …


The French Resolution, Jeremiah Farrell, Karen Farrell Jun 2019

The French Resolution, Jeremiah Farrell, Karen Farrell

Jeremiah Farrell

Jeremiah's puzzle "The French Resolution", which was exchanged at the 2017 International Puzzle Party in Paris, France. 100 puzzle designers create 100 copies of their puzzle and pass it out at the party and exchange them. This puzzle is also manufactured by Kadon Enterprises, Inc. as "The French Resolution".


You Like Japan, Jeremiah Farrell, Kate Jones Jun 2019

You Like Japan, Jeremiah Farrell, Kate Jones

Jeremiah Farrell

Jeremiah's puzzle "You Like Japan", which was exchanged at the 2016 International Puzzle Party in Kyoto, Japan, alongside Kate Jone's "You Like Japan" 12x12 Challenge. 100 puzzle designers create 100 copies of their puzzle and pass it out at the party and exchange them. This puzzle is manufacured by Kadon Enterprises, Inc. as "You Like Japan".


6 Cara Mudah Merontokan Benjolan Kasar Di Vagina Paling Manjur, Kutil Kelamin Ampuh Jun 2019

6 Cara Mudah Merontokan Benjolan Kasar Di Vagina Paling Manjur, Kutil Kelamin Ampuh

kutil kelamin Ampuh

6 Cara Mudah Merontokan Benjolan Kasar Di Vagina Paling Manjur-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belummengetahui tentang tumbuh daging dikemaluan.aging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil biasanya tumbuh …


6 Cara Mudah Penanganan Benjolan Kasar Di Vagina Paling Ampuh, Kutil Kelamin Ampuh Jun 2019

6 Cara Mudah Penanganan Benjolan Kasar Di Vagina Paling Ampuh, Kutil Kelamin Ampuh

kutil kelamin Ampuh

6 Cara Mudah Penanganan Benjolan Kasar Di Vagina Paling Ampuh-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belummengetahui tentang tumbuh daging dikemaluan.aging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil biasanya tumbuh …


5 Tips Mudah Penanganan Benjolan Kasar Di Vagina Paling Ampuh, Kutil Kelamin Ampuh Jun 2019

5 Tips Mudah Penanganan Benjolan Kasar Di Vagina Paling Ampuh, Kutil Kelamin Ampuh

kutil kelamin Ampuh

5 Tips Mudah Penanganan Benjolan Kasar Di Vagina Paling Ampuh-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belummengetahui tentang tumbuh daging dikemaluan.aging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil biasanya tumbuh …


7 Cara Mudah Menyembuhkan Daging Tumbuh Di Penis Terbukti Manjur, Kutil Kelamin Ampuh Jun 2019

7 Cara Mudah Menyembuhkan Daging Tumbuh Di Penis Terbukti Manjur, Kutil Kelamin Ampuh

kutil kelamin Ampuh

7 Cara Mudah Menyembuhkan Daging Tumbuh Di Penis Terbukti Manjur-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belum mengetahui tentang tumbuh daging dikemaluan.Daging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil biasanya …


5 Cara Mudah Mengatasi Daging Tumbuh Di Miss V Tanpa Oprasi, Kutil Kelamin Ampuh Jun 2019

5 Cara Mudah Mengatasi Daging Tumbuh Di Miss V Tanpa Oprasi, Kutil Kelamin Ampuh

kutil kelamin Ampuh

5 Cara Mudah Mengatasi Daging Tumbuh Di Miss V Tanpa Oprasi-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belum mengetahui tentang tumbuh daging dikemaluan.Daging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil …


History Of Chiropractic Care, Nerti Gedra Jun 2019

History Of Chiropractic Care, Nerti Gedra

nerti gedra

The practice of keeping the body healthy by sustaining an appropriately aligned spinal column may have its roots in the ancient times where Greeks and Chinese wrote about spinal Tulsa Chiropractor manipulations to ease body pain. Nevertheless, it was only after Daniel David Palmer of Iowa learned about spine adjustments that modern-day chiropractic care was born.

In 1895, D.D. Palmer did the first ever chiropractic adjustment on Harvey Lillard who became partially deaf following a "pop" in his back while working in a confined space. Upon examination, Palmer found a sore lump he thought was from a spinal misalignment that …


Phase Retrieval Using A Genetic Algorithm On The Systematic Image-Based Optical Alignment Test Bed, Jaime R. Taylor Jun 2019

Phase Retrieval Using A Genetic Algorithm On The Systematic Image-Based Optical Alignment Test Bed, Jaime R. Taylor

Dr. Jaime R. Taylor

NASA’s Marshall Space Flight Center’s Systematic Image-Based Optical Alignment (SIBOA) Testbed was developed to test phase retrieval algorithms and hardware techniques. Individuals working with the facility developed the idea of implementing phase retrieval by breaking the determination of the tip/tilt of each mirror apart from the piston motion (or translation) of each mirror. Presented in this report is an algorithm that determines the optimal phase correction associated only with the piston motion of the mirrors.


New Tests Of General Relativity, Quentin Bailey Jun 2019

New Tests Of General Relativity, Quentin Bailey

Quentin Bailey

The last decade has seen a rapid increase in the number of precision tests of relativity. This research has been motivated by the intriguing possibility that tiny deviations from relativity might arise in the underlying theory that is widely believed to successfully mesh General Relativity (GR) with quantum physics. Many of these tests have been analyzed within an effective field theory framework which generically describes possible deviations from exact relativity and contains some traditional test frameworks as limiting cases. One part of the activity has been a resurgence of interest in tests of relativity in the Minkowski-spacetime context, where Lorentz …


What Do We Know About Lorentz Symmetry?, Q. G. Bailey Jun 2019

What Do We Know About Lorentz Symmetry?, Q. G. Bailey

Quentin Bailey

Precision tests of Lorentz symmetry have become increasingly of interest to the broader gravitational and high-energy physics communities. In this talk, recent work on violations of local Lorentz invariance in gravity is discussed, including recent analysis constraining Lorentz violation in a variety of gravitational tests. The arena of short-range tests of gravity is highlighted, demonstrating that such tests are sensitive to a broad class of unexplored signals that depend on sidereal time and the geometry of the experiment.


Tests Of Lorentz Symmetry In The Gravitational Sector, Aurélien Hees, Quentin G. Bailey, Adrien Bourgoin, Hélène Pihan-Le Bars, Christine Guerlin, Christophe Le Poncin-Lafitte Jun 2019

Tests Of Lorentz Symmetry In The Gravitational Sector, Aurélien Hees, Quentin G. Bailey, Adrien Bourgoin, Hélène Pihan-Le Bars, Christine Guerlin, Christophe Le Poncin-Lafitte

Quentin Bailey

Lorentz symmetry is one of the pillars of both General Relativity and the Standard Model of particle physics. Motivated by ideas about quantum gravity, unification theories and violations of CPT symmetry, a significant effort has been put the last decades into testing Lorentz symmetry. This review focuses on Lorentz symmetry tests performed in the gravitational sector. We briefly review the basics of the pure gravitational sector of the Standard-Model Extension (SME) framework, a formalism developed in order to systematically parametrize hypothetical violations of the Lorentz invariance. Furthermore, we discuss the latest constraints obtained within this formalism including analyses of the …


Prospects For Sme Tests With Experiments At Syrte And Lkb, C. Guerlin, H. Pihan-Le Bars, Q. G. Bailey, P. Wolf Jun 2019

Prospects For Sme Tests With Experiments At Syrte And Lkb, C. Guerlin, H. Pihan-Le Bars, Q. G. Bailey, P. Wolf

Quentin Bailey

Preliminary work has been done in order to assess the perspectives of metrology and fundamental physics atomic experiments at SYRTE and LKB in the search for physics beyond the Standard Model and General Relativity. The first studies we identified are currently ongoing with the Microscope mission and with a Cs fountain clock. The latter brings significant improvement on the proton-sector coefficient cTT down to the 10−17 GeV level.


Improved Tests Of Lorentz Invariance In The Matter Sector Using Atomic Clocks, H. Pihan-Le Bars, C. Guerlin, Q. G. Bailey, S. Bize, P. Wolf Jun 2019

Improved Tests Of Lorentz Invariance In The Matter Sector Using Atomic Clocks, H. Pihan-Le Bars, C. Guerlin, Q. G. Bailey, S. Bize, P. Wolf

Quentin Bailey

For the purpose of searching for Lorentz-invariance violation in the minimal Standard-Model Extension, we perfom a reanalysis of data obtained from the 133Cs fountain clock operating at SYRTE. The previous study led to new limits on eight components of the ˜cµν tensor, which quantifies the anisotropy of the proton’s kinetic energy. We recently derived an advanced model for the frequency shift of hyperfine Zeeman transition due to Lorentz violation and became able to constrain the ninth component, the isotropic coefficient c˜TT, which is the least well-constrained coefficient of ˜cµν. This model is based on a second-order boost Lorentz transformation from …


Lorentz-Violating Electromagnetostatics, Quentin G. Bailey Jun 2019

Lorentz-Violating Electromagnetostatics, Quentin G. Bailey

Quentin Bailey

In this talk, the stationary limit of Lorentz-violating electrodynamics is discussed. As illustrated by some simple examples, the general solution includes unconventional mixing of electrostatic and magnetostatic effects. I discuss a high-sensitivity null-type measurement, exploiting Lorentz-violating electromagnetostatic effects, that could improve existing limits on parity-odd coefficients for Lorentz violation in the photon sector.


Constraints On Sme Coefficients From Lunar Laser Ranging, Very Long Baseline Interferometry, And Asteroid Orbital Dynamics, C. Le Poncin-Lafitte, A. Bourgoin, A. Hees, S. Bouquillon, S. Lambert, Q. G. Bailey, Et Al. Jun 2019

Constraints On Sme Coefficients From Lunar Laser Ranging, Very Long Baseline Interferometry, And Asteroid Orbital Dynamics, C. Le Poncin-Lafitte, A. Bourgoin, A. Hees, S. Bouquillon, S. Lambert, Q. G. Bailey, Et Al.

Quentin Bailey

Lorentz symmetry violations can be parametrized by an effective field theory framework that contains both General Relativity and the Standard Model of particle physics, called the Standard-Model Extension or SME. We consider in this work only the pure gravitational sector of the minimal SME. We present new constraints on the SME coefficients obtained from lunar laser ranging, very long baseline interferometry, and planetary motions.


Gravity Sector Of The Sme, Q. G. Bailey Jun 2019

Gravity Sector Of The Sme, Q. G. Bailey

Quentin Bailey

In this talk, the gravity sector of the effective field theory description of local Lorentz violation is discussed, including minimal and nonminimal curvature couplings. Also, recent experimental and observational analyses including solar-system ephemeris and short-range gravity tests are reviewed.


Intriguing Facts I Bet You Never Knew About Keto Tone France, John Torey Jun 2019

Intriguing Facts I Bet You Never Knew About Keto Tone France, John Torey

John Torey

Eating healthy is the bottom line if you want to feel good and look fine!

We comprehend what it resembles to attempt to shed pounds without a great deal of results. It can feel like you're never going to go anyplace, and you're never going to get the accomplishment you merit. Also, the most noticeably awful part is that it makes you feel insufficient.

Since, you could attempt each activity plan, each eating routine – and not get the outcomes you need. All in all, shouldn't something be said about the enhancements out there? Is it accurate to say that …


New Technique Of Stereolithography To Local Curing In Thermosensitive Resins Using Co(2) Laser, A. L.J. Munhoz, P. R.S. Wagner, M. C.F. Ierardi, A. E. Kiel, M. A.F. Scarparo, S. D. Allen Jun 2019

New Technique Of Stereolithography To Local Curing In Thermosensitive Resins Using Co(2) Laser, A. L.J. Munhoz, P. R.S. Wagner, M. C.F. Ierardi, A. E. Kiel, M. A.F. Scarparo, S. D. Allen

Susan D. Allen

A theoretical and experimental study of thermosensitive resins used in thermal stereolithography is presented. The process of local curing through the application of infrared radiation, which has proved to be useful in a new technique for the making of prototypes by means of selective heating with C02 laser (10.6um), is studied. The ideal composition of the thermosensitive resins has proved to be 10 parts epoxy, 1.4 part diethylene triamine (the curing agent) and 0.7 part silica powder. A physical theoretical model is applied for control of the parameters which influence the confinement of the curing in the irradiated bulk. A …


Penis Dan Anus Di Tumbuhi Bintik Kasar Seperti Kutil Apa Obatnya, Alif Nur Jun 2019

Penis Dan Anus Di Tumbuhi Bintik Kasar Seperti Kutil Apa Obatnya, Alif Nur

Alif Nur

penis dan anus di tumbuhi bintik kasar seperti kutil apa obatnya     Keseriusan tak hanya membutuhkan kefokusan dan kerja namun juga harus cerdas dalam menganalisa dan mengetahui apa yang akan ia lakukan dan kerjakan. Mereka yang berhasil sebenarnya mereka tak hanya cerdas dan berkopeten tinggi kebanyakan dari mereka yang sukses karena mereka tau bagaimana pait dan sakitnya terjatuh berkali kali sehinga membuat mereka lebih berhati hati dan selalu memperhitungkan langka baik mereka. untuk mengatasi bintik kecil kasar mirip kutil di penis 


5 Cara Mudah Menyembuhkan Daging Tumbuh Di Vagina Terbukti Manjur, Kutil Kelamin Ampuh Jun 2019

5 Cara Mudah Menyembuhkan Daging Tumbuh Di Vagina Terbukti Manjur, Kutil Kelamin Ampuh

kutil kelamin Ampuh

5 Cara Mudah Menyembuhkan Daging Tumbuh Di Vagina Terbukti Manjur-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belummengetahui tentang tumbuh daging dikemaluan.aging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil biasanya tumbuh …


6 Tips Mudah Mengatasi Daging Tumbuh Di Penis Tanpa Oprasi, Kutil Kelamin Ampuh Jun 2019

6 Tips Mudah Mengatasi Daging Tumbuh Di Penis Tanpa Oprasi, Kutil Kelamin Ampuh

kutil kelamin Ampuh

6 Tips Mudah Mengatasi Daging Tumbuh Di Penis Tanpa Oprasi-Mungkin Anda sedang mencari cara yang mudah untuk mengobati daging tumbuh di kemaluan.banyak orang yang belum mengetahui tentang tumbuh daging dikemaluan.Daging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, kutil biasanya …


6 Tips Mudah Mengobati Benjolan Kasar Di Miss V Terbukti Ampuh, Kutil Kelamin Ampuh Jun 2019

6 Tips Mudah Mengobati Benjolan Kasar Di Miss V Terbukti Ampuh, Kutil Kelamin Ampuh

kutil kelamin Ampuh

6 Tips Mudah Mengobati Benjolan Kasar Di Miss V Terbukti Ampuh-Apakah anda seseorang yang sedang mencari tips mudah untuk mengobati daging tumbuh di miss v yang sangat mengganggu aktivitas anda.Daging tumbuh di kemaluan merupakan penyakit dari kutil kelamin.mungkin anda adalah salah satunya yang terkena kutil kelamin.kutil kelamin adalah salah satu penyakit dengan gejala yang paling umum muncul akibat infeksi menular seksual. Kondiloma akuminata umumnya muncul akibat infeksi HPV (human papillomavirus) tertentu, yaitu HPV 6 dan  11.Kondiloma akuminata berbentuk benjolan daging kecil berwarna merah atau bergerombol banyak yang tampak seperti kembang kol yang tumbuh di sekitar kelamin. Dalam banyak kasus, …


Parallel Genome-Wide Expression Profiling Of Host And Pathogen During Soybean Cyst Nematode Infection Of Soybean, Nagabhushana Ithal, Justin Recknor, Dan Nettleton, Leonard Hearne, Tom Maier, Thomas J. Baum, Melissa G. Mitchum Jun 2019

Parallel Genome-Wide Expression Profiling Of Host And Pathogen During Soybean Cyst Nematode Infection Of Soybean, Nagabhushana Ithal, Justin Recknor, Dan Nettleton, Leonard Hearne, Tom Maier, Thomas J. Baum, Melissa G. Mitchum

Dan Nettleton

Global analysis of gene expression changes in soybean (Glycine max) and Heterodera glycines (soybean cyst nematode [SCN]) during the course of infection in a compatible interaction was performed using the Affymetrix GeneChip soybean genome array. Among 35,611 soybean transcripts monitored, we identified 429 genes that showed statistically significant differential expression between uninfected and nematode-infected root tissues. These included genes encoding enzymes involved in primary metabolism; biosynthesis of phenolic compounds, lignin, and flavonoids; genes related to stress and defense responses; cell wall modification; cellular signaling; and transcriptional regulation. Among 7,431 SCN transcripts monitored, 1,850 genes showed statistically significant differential …


Sequence Mining And Transcript Profiling To Explore Cyst Nematode Parasitism, Axel A. Elling, Makedonka Mitreva, Xiaowu Gai, John Martin, Justin Recknor, Eric L. Davis, Richard S. Hussey, Dan Nettleton, James P. Mccarter, Thomas J. Baum Jun 2019

Sequence Mining And Transcript Profiling To Explore Cyst Nematode Parasitism, Axel A. Elling, Makedonka Mitreva, Xiaowu Gai, John Martin, Justin Recknor, Eric L. Davis, Richard S. Hussey, Dan Nettleton, James P. Mccarter, Thomas J. Baum

Dan Nettleton

Background: Cyst nematodes are devastating plant parasites that become sedentary within plant roots and induce the transformation of normal plant cells into elaborate feeding cells with the help of secreted effectors, the parasitism proteins. These proteins are the translation products of parasitism genes and are secreted molecular tools that allow cyst nematodes to infect plants.

Results: We present here the expression patterns of all previously described parasitism genes of the soybean cyst nematode, Heterodera glycines, in all major life stages except the adult male. These insights were gained by analyzing our gene expression dataset from experiments using the Affymetrix Soybean …


Mu Transposon Insertion Sites And Meiotic Recombination Events Co-Localize With Epigenetic Marks For Open Chromatin Across The Maize Genome, Sanzhen Liu, Cheng-Ting Yeh, Tieming Ji, Kai Ying, Haiyan Wu, Ho Man Tang, Yan Fu, Daniel S. Nettleton, Patrick S. Schnable Jun 2019

Mu Transposon Insertion Sites And Meiotic Recombination Events Co-Localize With Epigenetic Marks For Open Chromatin Across The Maize Genome, Sanzhen Liu, Cheng-Ting Yeh, Tieming Ji, Kai Ying, Haiyan Wu, Ho Man Tang, Yan Fu, Daniel S. Nettleton, Patrick S. Schnable

Dan Nettleton

The Mu transposon system of maize is highly active, with each of the ∼50–100 copies transposing on average once each generation. The approximately one dozen distinct Mutransposons contain highly similar ∼215 bp terminal inverted repeats (TIRs) and generate 9-bp target site duplications (TSDs) upon insertion. Using a novel genome walking strategy that uses these conserved TIRs as primer binding sites, Mu insertion sites were amplified from Mu stocks and sequenced via 454 technology. 94% of ∼965,000 reads carried Mu TIRs, demonstrating the specificity of this strategy. Among these TIRs, 21 novel Mu TIRs were discovered, revealing additional complexity of …


Identifying Differentially Expressed Genes In Unreplicated Multiple-Treatment Microarray Timecourse Experiments, Rhonda Decook, Dan Nettleton, Carol Foster, Eve S. Wurtele Jun 2019

Identifying Differentially Expressed Genes In Unreplicated Multiple-Treatment Microarray Timecourse Experiments, Rhonda Decook, Dan Nettleton, Carol Foster, Eve S. Wurtele

Dan Nettleton

Microarray technology has become widespread as a means to investigate gene function and metabolic pathways in an organism. A common experiment involves probing, at each of several time points, the gene expression of experimental units subjected to different treatments. Due to the high cost of microarrays, such experiments may be performed without replication and therefore provide a gene expression measurement of only one experimental unit for each combination of treatment and time point. Though an experiment with replication would provide more powerful conclusions, it is still possible to identify differentially expressed genes and to estimate the number of false positives …