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2006

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Articles 3631 - 3660 of 5872

Full-Text Articles in Physical Sciences and Mathematics

Water-Quality And -Quantity Data For Abandoned Underground Coal Mines In Eastern Kentucky, Steven E. Webb, Dennis H. Cumbie, James S. Dinger, Leslie K. Russo Jan 2006

Water-Quality And -Quantity Data For Abandoned Underground Coal Mines In Eastern Kentucky, Steven E. Webb, Dennis H. Cumbie, James S. Dinger, Leslie K. Russo

Information Circular--KGS

Water-quality and -quantity analyses were performed between 1997 and 2003 by the Kentucky Geological Survey under contract from the Kentucky River Authority and the Kentucky Infrastructure Authority to study abandoned underground coal mines as possible water supplies for communities in the Eastern Kentucky Coal Field. The steep terrain of the coal field limits surface-water supplies, and groundwater systems are difficult to locate and often have too low a yield to provide community water supplies. KGS has been working with the Kentucky River Authority, the Kentucky Infrastructure Authority, and local government officials to search for water supplies in abandoned underground coal …

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Oxygen Depletion In The Gulf Of Mexico Adjacent To The Mississippi River, Nancy N. Rabalais, R. Eugene Turner Jan 2006

Oxygen Depletion In The Gulf Of Mexico Adjacent To The Mississippi River, Nancy N. Rabalais, R. Eugene Turner

Faculty Publications

The seasonal formation of a bottom water layer severely depleted in dissolved oxygen has become a perennial occurrence on the Louisiana continental shelf adjacent to the Mississippi River system. Dramatic changes have occurred in this coastal ecosystem in the last half of the 20th century as the loads of dissolved inorganic nitrogen tripled. There are increases in primary production, shifts in phytoplankton community composition, changes in trophic interactions, and worsening severity of hypoxia. The hypoxic conditions (dissolved oxygen less than 2 mg l-1) cover up to 22,000 km2 of the seabed in mid-summer. Dissolved oxygen concentrations seldom decrease to anoxia, …

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On Self-Adjoint And J-Self-Adjoint Dirac-Type Operators: A Case Study, Stephen L. Clark, Fritz Gesztesy Jan 2006

On Self-Adjoint And J-Self-Adjoint Dirac-Type Operators: A Case Study, Stephen L. Clark, Fritz Gesztesy

Mathematics and Statistics Faculty Research & Creative Works

We provide a comparative treatment of some aspects of spectral theory for self-adjoint and non-self-adjoint (but J-self-adjoint) Dirac-type operators connected with the defocusing and focusing nonlinear Schrödinger equation, of relevance to nonlinear optics. In addition to a study of Dirac and Hamiltonian systems, we also introduce the concept of Weyl-Titchmarsh half-line m-coefficients (and 2 × 2 matrix-valued M-matrices) in the non-self-adjoint context and derive some of their basic properties. We conclude with an illustrative example showing that crossing spectral arcs in the non-self-adjoint context imply the blowup of the norm of spectral projections in the limit where the crossing point …

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Multiple Lebesgue Integration On Time Scales, Gusein Sh. Guseinov, Martin Bohner Jan 2006

Multiple Lebesgue Integration On Time Scales, Gusein Sh. Guseinov, Martin Bohner

Mathematics and Statistics Faculty Research & Creative Works

We study the process of multiple Lebesgue integration on time scales. The relationship of the Riemann and the Lebesgue multiple integrals is investigated.

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Singular Second-Order Multipoint Dynamic Boundary Value Problems With Mixed Derivatives, Hua Luo, Martin Bohner Jan 2006

Singular Second-Order Multipoint Dynamic Boundary Value Problems With Mixed Derivatives, Hua Luo, Martin Bohner

Mathematics and Statistics Faculty Research & Creative Works

We study a certain singular second-order m-point boundary value problem on a time scale and establish the existence of a solution. The proof of our main result is based upon the Leray-Schauder continuation theorem.

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Boundedness In Functional Dynamic Equations On Time Scales, Elvan Akin, Youssef N. Raffoul Jan 2006

Boundedness In Functional Dynamic Equations On Time Scales, Elvan Akin, Youssef N. Raffoul

Mathematics and Statistics Faculty Research & Creative Works

Using nonnegative definite Lyapunov functionals, we prove general theorems for the boundedness of all solutions of a functional dynamic equation on time scales. We apply our obtained results to linear and nonlinear Volterra integro-dynamic equations on time scales by displaying suitable Lyapunov functionals.

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Modeling Of Bioinspired Sensors For Flow Separation Detection For Micro Air Vehicles, Belinda A. Batten, John R. Singler, Benjamin T. Dickinson Jan 2006

Modeling Of Bioinspired Sensors For Flow Separation Detection For Micro Air Vehicles, Belinda A. Batten, John R. Singler, Benjamin T. Dickinson

Mathematics and Statistics Faculty Research & Creative Works

Autonomous micro air vehicle (MAV) flight faces inherent stability challenges. One challenge is controlling flow separation over the airfoil and an autonomous control system for MAV flight may be enhanced with closed loop separation control. In this work, we focus on modeling biologically inspired hair cell sensors for future flow control applications. We model the sensor output and present examples and numerical results.

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Feedback Control Of Low Dimensional Models Of Transition To Turbulence, John A. Burns, John R. Singler Jan 2006

Feedback Control Of Low Dimensional Models Of Transition To Turbulence, John A. Burns, John R. Singler

Mathematics and Statistics Faculty Research & Creative Works

The problem of controlling or delaying transition to turbulence in shear flows has been the subject of numerous papers over the past twenty years. This period has seen the development of several low dimensional models for parallel shear flows in an attempt to explain the failure of classical linear hydrodynamic stability theory to correctly predict transition. In recent years, ideas from robust control theory have been employed to attack this problem. In this paper we use these models to develop a scenario for transition that employs both classical bifurcation theory and robust control theory. In addition, we present numerical results …

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The Poweratlas: A Power And Sample Size Atlas For Microarray Experimental Design And Research, Grier P. Page, Jode W. Edwards, Gary L. Gadbury, Prashanth Yelisetti, Jelai Wang, Prinal Trivedi, David B. Allison Jan 2006

The Poweratlas: A Power And Sample Size Atlas For Microarray Experimental Design And Research, Grier P. Page, Jode W. Edwards, Gary L. Gadbury, Prashanth Yelisetti, Jelai Wang, Prinal Trivedi, David B. Allison

Mathematics and Statistics Faculty Research & Creative Works

Microarrays permit biologists to simultaneously measure the mRNA abundance of thousands of genes. An important issue facing investigators planning microarray experiments is how to estimate the sample size required for good statistical power. What is the projected sample size or number of replicate chips needed to address the multiple hypotheses with acceptable accuracy? Statistical methods exist for calculating power based upon a single hypothesis, using estimates of the variability in data from pilot studies. There is, however, a need for methods to estimate power and/or required sample sizes in situations where multiple hypotheses are being tested, such as in microarray …

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The Emergence Of Large-Scale Coherent Structure Under Small-Scale Random Bombardments, Andrew Majda, Xiaoming Wang Jan 2006

The Emergence Of Large-Scale Coherent Structure Under Small-Scale Random Bombardments, Andrew Majda, Xiaoming Wang

Mathematics and Statistics Faculty Research & Creative Works

We provide mathematical justification of the emergence of large-scale coherent structure in a two-dimensional fluid system under small-scale random bombardments with small forcing and appropriate scaling assumptions. the analysis shows that the large-scale structure emerging out of the small-scale random forcing is not the one predicted by equilibrium statistical mechanics. But the error is very small, which explains earlier successful prediction of the large-scale structure based on equilibrium statistical mechanics. © 2005 Wiley Periodicals, Inc.

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Dynamics Of Rotating Bose-Einstein Condensates And Its Efficient And Accurate Numerical Computation, Weizhu Bao, Qiang Du, Yanzhi Zhang Jan 2006

Dynamics Of Rotating Bose-Einstein Condensates And Its Efficient And Accurate Numerical Computation, Weizhu Bao, Qiang Du, Yanzhi Zhang

Mathematics and Statistics Faculty Research & Creative Works

In this paper, we study the dynamics of rotating Bose--Einstein condensates (BEC) based on the Gross--Pitaevskii equation (GPE) with an angular momentum rotation term and present an efficient and accurate algorithm for numerical simulations. We examine the conservation of the angular momentum expectation and the condensate width and analyze the dynamics of a stationary state with a shift in its center. By formulating the equation in either the two-dimensional polar coordinate system or the three-dimensional cylindrical coordinate system, the angular momentum rotation term becomes a term with constant coefficients. This allows us to develop an efficient time-splitting method which is …

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The Hurwitz Zeta Function As A Convergent Series, Roman Dwilewicz, Jan Minac Jan 2006

The Hurwitz Zeta Function As A Convergent Series, Roman Dwilewicz, Jan Minac

Mathematics and Statistics Faculty Research & Creative Works

New series for the Hurwitz zeta function which converge on the whole plane, except s = 1, are developed. This is applied to obtain a remarkably simple evaluation of some special values of the function.

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A Peano-Akô Type Theorem For Variational Inequalities, Vy Khoi Le Jan 2006

A Peano-Akô Type Theorem For Variational Inequalities, Vy Khoi Le

Mathematics and Statistics Faculty Research & Creative Works

We consider in this paper a Peano-Akô property of solution sets in some quasilinear elliptic variational inequalities. As consequences, variants of that property and a partial Hukuhara-Kneser theorem for inequalities are derived.

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Sponges Of Navassa, Janie L. Wulff, Timothy D. Swain Jan 2006

Sponges Of Navassa, Janie L. Wulff, Timothy D. Swain

Marine & Environmental Sciences Faculty Reports

This photographic guide was compiled from data collected during the 2004 NOAA survey of the coral reefs of Navassa and does not represent a comprehensive list of all Porifera in Navassa. Specifically missing are taxa that inhabit caves, overhangs, vertical walls; species that live in the interstices of the reef framework; and species found at depths greater than 50 meters. Specimens were identified by Janie Wulff and Timothy Swain of Florida State University using a combination of digital photography, field observations, and microscopic examination of siliceous spicules. Genera are organized into higher taxa according to Systema Porifera, Hooper & van …

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An Experimental Histopathological Rating Scale Of Sedimentation Stress In The Caribbean Coral Montastraea Cavernosa, Bernardo Vargas-Ángel, Bernhard Riegl, David S. Gilliam, Richard E. Dodge Jan 2006

An Experimental Histopathological Rating Scale Of Sedimentation Stress In The Caribbean Coral Montastraea Cavernosa, Bernardo Vargas-Ángel, Bernhard Riegl, David S. Gilliam, Richard E. Dodge

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Colonies of Montastraea cavernosa were exposed to daily applications of approx. 200–225 mg cm– 2 sediments, during a four-week period, in order to investigate coral responses to increased sedimentation. Effects were assessed based on the histopathological condition of specimen tissues. Mild stress responses were noted as early as week one, including tissue swelling and polyp retraction, as well as changes in size and appearance of mucous secretory cells. As stress progressed, further inflammation of the mucous secretory cells was observed. Severe effects were observed in weeks 3 and 4, including atrophy of the outer epidermis, increased granularity and debris in …

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Visual_Hea: Habitat Equivalency Analysis Software To Calculate Compensatory Restoration Following Natural Resource Injury, Kevin E. Kohler, Richard E. Dodge Jan 2006

Visual_Hea: Habitat Equivalency Analysis Software To Calculate Compensatory Restoration Following Natural Resource Injury, Kevin E. Kohler, Richard E. Dodge

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Habitat Equivalency Analysis (HEA) is a means to determine the amount of compensatory restoration required to provide services that are equivalent to the interim loss of natural resource services following an injury. HEA includes a discounting procedure to account for asset valuation in that the total asset value is equal to the discounted value of the future stream of all services from the natural resource or the compensatory resource. Discounting is used to include the relative valuation of loss and gain of ecological services of the resources over time. Visual_HEA is a computer program developed to calculate the amount of …

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Growth And Survivorship Of Scleractinian Coral Transplants And The Effectiveness Of Plugging Core Holes In Transplant Donor Colonies, Elizabeth Glynn Fahy, Richard E. Dodge, Daniel P. Fahy, T. Patrick Quinn, David S. Gilliam, Richard E. Spieler Jan 2006

Growth And Survivorship Of Scleractinian Coral Transplants And The Effectiveness Of Plugging Core Holes In Transplant Donor Colonies, Elizabeth Glynn Fahy, Richard E. Dodge, Daniel P. Fahy, T. Patrick Quinn, David S. Gilliam, Richard E. Spieler

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Replicate scleractinian coral transplants were obtained from the species Meandrina meandrites and Montastrea cavernosa on a natural reef, off Dania Beach, Florida, using a hydraulic drill fitted with a 4 in. (~10 cm) core barrel. The transplants were fixed to Reef Ball™ substrates using an adhesive marine epoxy. Drill holes in the donor corals (core holes) were filled with concrete plugs. Control corals, of comparable size to both donor colonies and transplant corals, were monitored for comparison. Transplant corals, donor corals, and controls on the natural reef were monitored for growth and survivorship. Core holes were monitored for tissue regrowth …

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Spatial Patterns In Arabian Gulf Coral Assemblages (Jebel Ali, Dubai, U.A.E.) In Response To Temperature-Forcing, Bernhard Riegl, Samuel J. Purkis, Kevin E. Kohler, Richard E. Dodge Jan 2006

Spatial Patterns In Arabian Gulf Coral Assemblages (Jebel Ali, Dubai, U.A.E.) In Response To Temperature-Forcing, Bernhard Riegl, Samuel J. Purkis, Kevin E. Kohler, Richard E. Dodge

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

We evaluated spatial and temporal patterns using maps from Ikonos satellite imagery in combination with 8 years of line transects and photosquares and the HadISST1 sea-surface temperature data set to explain why coral assemblages in the southern Arabian Gulf (Dubai) are impoverished and mostly do not build framework reefs. Analysis of archive sea surface temperature (SST) data confirms that the area is subjected to recurrent temperature anomalies. Frequencies of anomalies might suggest at least a partial link to the El Niño Southern Oscillation possibly via the Indian Ocean Zonal Mode. The dominant driver of local temperature was oscillations in the …

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Fractal Patterns Of Coral Communities: Evidence From Remote Sensing (Arabian Gulf, Dubai, U.A.E.), Samuel J. Purkis, Bernhard Riegl, Richard E. Dodge (Editor) Jan 2006

Fractal Patterns Of Coral Communities: Evidence From Remote Sensing (Arabian Gulf, Dubai, U.A.E.), Samuel J. Purkis, Bernhard Riegl, Richard E. Dodge (Editor)

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

In this study, the spatial character of benthic communities is investigated in an Arabian Gulf shallow subtidal carbonate ramp setting, using IKONOS satellite imagery. The patchy distribution of three assemblages of live and dead corals on extensive (but also fragmented) hardground pavements was investigated using a variety of spatial statistics. It was found that the spatial expression of the benthic groups display characteristics that approximate to power-law distributions over several orders of magnitude to an extent that suggests fractal behaviour. Pronounced anisotropy was observed between the spatial patterns in the near-shore and off-shore region which is attributed to different mechanisms …

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Implications Of Natural Variation Of Fish Assemblages To Coral Reef Management, Lance K. B. Jordan, Richard E. Spieler Jan 2006

Implications Of Natural Variation Of Fish Assemblages To Coral Reef Management, Lance K. B. Jordan, Richard E. Spieler

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

In order to evaluate the impact of a planned beach renourishment project we obtained baseline data on hardbottom coral reef fish assemblages adjacent to the beach. During the summers of 2001 and 2003 we conducted 92 and 89 visual transect-counts, respectively, each 30x2x1m. Fish were recorded by species, abundance, and size class. Fish exhibited a 62.8% decrease in abundance between the 2001 and 2003 surveys. Juvenile Haemulon spp. (<5cm total length, TL) alone decreased 72.8% in abundance and, due to their predominance, primarily drove the decrease seen for total abundance. The second most abundant species Halichoeres bivittatus, also primarily juveniles (<5cm TL), contributed 8% of the total abundance and also showed a significant decrease between the 2001 and 2003 surveys. Even after removal of the two most abundant taxa, total abundance remained significantly lower the second survey year, suggesting the possibility of a community-wide decrease in abundance. However, the decrease between years was mainly due to a decrease in juveniles. When fish less than 5cm TL were removed from the dataset, no significant difference in abundance between years was detected. Species richness also declined significantly with fewer species noted in 2003 and eight fewer total species between years. Nonetheless, multivariate examination of assemblage structure did not indicate a difference between years. These results have important implications for determining potential anthropogenic change in fish assemblages (e.g. caused by beach renourishment).

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Impacts Of A Manure Composting Program On Stream Water Quality, A. Bekele, A. M.S. Mcfarland, A. J. Whisenant Jan 2006

Impacts Of A Manure Composting Program On Stream Water Quality, A. Bekele, A. M.S. Mcfarland, A. J. Whisenant

Faculty Publications

In February 2001, the Texas Commission on Environmental Quality (TCEQ) adopted a Total Maximum Daily Load (TMDL) for soluble reactive phosphorus (SRP) along the North Bosque River. Within this TMDL, dairy waste application fields were identified as the major nonpoint-source contribution of nutrients. In September 2000, a manure composting program was initiated that resulted in about 500,000 metric tons of dairy manure being hauled to composting facilities and exported from the watershed through December 2004. To evaluate the impact of the manure composting program on stream water quality, storm event mean concentrations of nutrients and total suspended solids were compared …

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Structure And Vibrational Spectra Of Mononitrated Benzo [A] Pyrenes., Kefa Karimu Onchoke, Christopher M. Hadad, Prabir K. Dutta Jan 2006

Structure And Vibrational Spectra Of Mononitrated Benzo [A] Pyrenes., Kefa Karimu Onchoke, Christopher M. Hadad, Prabir K. Dutta

Faculty Publications

The molecules benzo[a]pyrene (BaP) and 1-, 3-, and 6-nitrobenzo[a]pyrene (1-NBaP, 3-NBaP, 6-NBaP) are currently of significant interest due to their presence in respirable combustion exhaust particulates and their mutagenic and carcinogenic properties. Structure−function correlations as well as spectroscopic signatures for trace analysis are necessary for these benzo[a]pyrene derivatives. In this paper, detailed infrared and Raman spectroscopic data of BaP and its three mononitrated isomers are provided for the first time. By utilizing density functional theory (DFT, B3LYP method with 6-311+G** basis set), the molecular geometries and the vibrational spectra are calculated. Good agreement is …

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Spatio-Temporal Characteristics Of Point And Field Sources In Wireless Sensor Networks, Mehmet C. Vuran, Ӧzgür B. Akan Jan 2006

Spatio-Temporal Characteristics Of Point And Field Sources In Wireless Sensor Networks, Mehmet C. Vuran, Ӧzgür B. Akan

CSE Conference and Workshop Papers

Wireless Sensor Networks (WSN) are comprised of densely deployed sensor nodes collaboratively observing and communicating extracted information about a physical phenomenon. Dense deployment of sensor nodes makes the sensor observations highly correlated in the space domain. In addition, consecutive samples obtained by a sensor node are also temporally correlated for the applications involving the observation of the variation of a physical phenomenon. Based on the physical characteristics and dispersion pattern over the area, the phenomenon to be observed can be modeled as point source or field source. Clearly, understanding the spatio-temporal correlation characteristics of the point and field sources brings …

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Identifying Potential Conflict Associated With Oil And Gas Exploration In Texas State Coastal Waters: A Multi-Criteria Spatial Analysis, Samuel Brody, Himanshu Grover, Sarah Bernhardt, Zhenghong Tang, Bianca Whitaker, Colin Spence Jan 2006

Identifying Potential Conflict Associated With Oil And Gas Exploration In Texas State Coastal Waters: A Multi-Criteria Spatial Analysis, Samuel Brody, Himanshu Grover, Sarah Bernhardt, Zhenghong Tang, Bianca Whitaker, Colin Spence

Community and Regional Planning Program: Faculty Scholarly and Creative Activity

Recent interest in expanding offshore oil production within waters of the United States has been met with opposition by groups concerned with recreational, environmental, and aesthetic values associated with the coastal zone. Although the proposition of new oil platforms off the coast has generated conflict over how coastal resources should be utilized, little research has been conducted on where these user conflicts might be most intense and which sites might be most suitable for locating oil production facilities in light of the multiple, and often times, competing interests. In this article, we develop a multiple-criteria spatial decision support tool that …

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Truly Non Invasive Glucose Optical Sensor Based On Metal Nanoparticles Generation, Marisol Garcia Jan 2006

Truly Non Invasive Glucose Optical Sensor Based On Metal Nanoparticles Generation, Marisol Garcia

Electronic Theses and Dissertations

Diabetes is a disease that causes many complications in human normal function. This disease represents the sixth-leading cause of death in USA. Prevention of diabetes-related complications can be accomplished through tight control of glucose levels in blood. In the last decades many different glucose sensors have been developed, however, none of them are really non invasive. Herein, we present the study of the application of gold and silver nanoparticles with different shapes and aspect ratios to detect glucose traces in human fluids such as tears and sweat. This is to our knowledge the first truly non invasive glucose optical sensor, …

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A Preliminary Review Of Neotropical Primates In The Subsistence And Symbolism Of Indigenous Lowland South American Peoples, Loretta Cormier Jan 2006

A Preliminary Review Of Neotropical Primates In The Subsistence And Symbolism Of Indigenous Lowland South American Peoples, Loretta Cormier

Ecological and Environmental Anthropology (University of Georgia)

This article provides a review of selected literature of nonhuman primates in the subsistence and symbolism of indigenous lowland South American groups. While few works have focused specifically on the relationship between human and nonhuman primates in Amazonia and the surrounding areas, a number of ethnographic works do incorporate information about the roles of monkeys in varied groups. The section on subsistence focuses on the use of primates as food, including preferences, avoidances, and taboos. The section on symbolism focuses on the role of monkeys in myths, folklore, and in delineating the humanity/animality divide.

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Cluster-Assembled Nanocomposites, Yinfan Xu, M.L. Yan, David J. Sellmyer Jan 2006

Cluster-Assembled Nanocomposites, Yinfan Xu, M.L. Yan, David J. Sellmyer

David Sellmyer Publications

This chapter focuses on a gas-aggregation technique to prepare magnetic nanoclusters with controllable cluster sizes and size distributions. The review includes current research on nanoclusters, such as uncoated and oxide-coated Fe, Co and Fe clusters and clusters made from alloys, but special emphasis is on highly anisotropic L10-ordered FePt clusters, which are of potential interest for magnetic recording with ultrahigh areal densities of more than 1 Tera bit/in2. In particular, we discuss magnetic and structural properties of FePt nanoclusters and thin films. Another approach discussed in the chapter is to create cluster nanocomposites by multilayering …

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Search For R-Parity Violating Supersymmetry Via The Llē Couplings Λ121, Λ122 Or Λ133 In Pp Collisions At √ S = 1.96 Tev, V. M. Abazov, Kenneth A. Bloom, Gregory Snow, Do Collaboration Jan 2006

Search For R-Parity Violating Supersymmetry Via The Llē Couplings Λ121, Λ122 Or Λ133 In Pp Collisions At √ S = 1.96 Tev, V. M. Abazov, Kenneth A. Bloom, Gregory Snow, Do Collaboration

Kenneth Bloom Publications

A search for gaugino pair production with a trilepton signature in the framework of R-parity violating supersymmetry via the couplings λ121, λ122, or λ133 is presented. The data, corresponding to an integrated luminosity of L ≈ 360 pb−1, were collected from April 2002 to August 2004 with the D∅ detector at the Fermilab Tevatron Collider, at a center-of-mass energy of √s = 1.96 TeV. This analysis considers final states with three charged leptons with the flavor combinations eel, μμl, and eeτ (l = e or μ). No evidence for supersymmetry is …

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Refuge Update – January/February 2006, Volume 3, Number 1 Jan 2006

Refuge Update – January/February 2006, Volume 3, Number 1

RefugeUpdate (USFWS-NWRS)

Table of Contents:

Three New Species in Alaska
Conserving Wildlife State by State
Focus on…Incident Command
Mushing to Your New Job

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Sequence-Enabled Reassembly (Seer) Peptides For The Detection Of Dna Sequences, Aik T. Ooi, Cliff I. Stains, Jason R. Porter, Indraneel Ghosh, David J. Segal Jan 2006

Sequence-Enabled Reassembly (Seer) Peptides For The Detection Of Dna Sequences, Aik T. Ooi, Cliff I. Stains, Jason R. Porter, Indraneel Ghosh, David J. Segal

Cliff Stains Publications

By combining custom zinc finger (ZF) DNA-binding technology [1,2] with protein fragment complementation [3], we have developed a technology, designated SEER (Sequence-Enabled Reassembly), that has the potential to “see” or detect genetic information within a living cell (Figure 1). These agents consist of two inactive parts of signal-generating peptides that have the ability to recognize specific DNA sequences. The two parts bind near each other in the presence of a user-defined DNA target site and generate a fluorescent signal. Two prototype SEER systems have been constructed, based on the reassembly of green fluorescent protein (SEERGFP) [4] and the enzyme β-lactamase …

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