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Articles 2821 - 2850 of 12081

Full-Text Articles in Physical Sciences and Mathematics

An In Silico Dna Sequence Comparison Of The Perennial Ryegrass And Rice Genomes, Marty J. Faville Apr 2023

An In Silico Dna Sequence Comparison Of The Perennial Ryegrass And Rice Genomes, Marty J. Faville

IGC Proceedings (1993-2023)

Comparative mapping studies in the family Poaceae, which includes rice (a model species for this family) and perennial ryegrass (PRG) have indicated macro-colinearity of genes is generally conserved across different genomes. Genome mapping of simple sequence repeat markers derived from expressed sequence tags (EST-SSRs) for PRG (Faville et al., 2004) provides a vehicle for DNA sequence-based matching of mapped PRG genes to orthologous positions in the rice genome, which can be used to establish comparative relationships between these species’ genomes. We have initiated such an analysis using an EST-SSR-based PRG genome map. Our objective was to assess this in …

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The Feasibility Of Autoclave-Assisted Water Soluble Carbohydrate Extraction To Distinguish Annual Ryegrass Genotypes At The Seedling Stage, L. P. Passos, F. B. De Sousa, A. Mittelmann, M. C. Vidigal, I. G. Perry, L. O. Cruz, J. A. Magalhães Apr 2023

The Feasibility Of Autoclave-Assisted Water Soluble Carbohydrate Extraction To Distinguish Annual Ryegrass Genotypes At The Seedling Stage, L. P. Passos, F. B. De Sousa, A. Mittelmann, M. C. Vidigal, I. G. Perry, L. O. Cruz, J. A. Magalhães

IGC Proceedings (1993-2023)

Annual ryegrass (Lolium multiflorum) swards are being used increasingly in Southern Brazil as animal forage (Carvalho et al., 2001). As observed elsewhere, reduced forage availability and quality during dry or cold seasons often limits the efficacy of pasture utilisation. As reported by Meissner et al. (1992), this demands breeding efforts to improve forage nutritive value. However, lack of concurrent examination of large number of samples for important nutritional parameters, like water-soluble carbohydrate (WSC) levels, which could lead to more precise selection strategies, is a common constraint of those programs. This study aimed to verify the suitability …

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A Quantitative Trait Locus Analysis Of Root Distribution In Perennial Ryegrass (Lolium Perenne L.), Marty J. Faville, James R. Crush, H. S. Easton Apr 2023

A Quantitative Trait Locus Analysis Of Root Distribution In Perennial Ryegrass (Lolium Perenne L.), Marty J. Faville, James R. Crush, H. S. Easton

IGC Proceedings (1993-2023)

Root system architecture impacts perennial ryegrass performance, with deeper roots potentially contributing to drought tolerance, nutrient interception, and anchoring of plants. Root mass in a perennial ryegrass sward is typically shallow, concentrated in the top 10 cm of soil (Troughton 1957). Phenotypic selection for deeper root systems in breeding programmes is limited by the inaccessibility of underground plant components. We aim to use quantitative trait locus (QTL) analysis to discover genetic factors influencing root architecture traits, including vertical root distribution, in perennial ryegrass. Ultimately, markers linked to root architecture QTL may be used in a marker-assisted selection strategy that would …

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Variability And Correlations Of Some Investigated Traits Of Perennial Ryegrass Populations, D. Sokolovic, S. Ignjatovic, Z. Tomic Apr 2023

Variability And Correlations Of Some Investigated Traits Of Perennial Ryegrass Populations, D. Sokolovic, S. Ignjatovic, Z. Tomic

IGC Proceedings (1993-2023)

Perennial ryegrass (Lolium perenne) is one of the most important perennial forage grasses for temperate climates. It is a highly productive grass with the highest nutritive value (Sokolovi et al., 2002). In Serbia, breeders have developed perennial ryegrass cultivars with high stabile yield and quality with different times of maturity and resistance to drought and frost. The initial breeding material were usually wild populations (Charmet et. al., 1996) with high variability and adaptability. These characteristics lend themselves for selection of superior genotypes. But breeding for some important agronomic traits may influence others. This relationship between traits …

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Cattle Production From Native Pastures In The Semi-Humid Grasslands Of Uganda, S. Okello, Elly N. Sabiiti Apr 2023

Cattle Production From Native Pastures In The Semi-Humid Grasslands Of Uganda, S. Okello, Elly N. Sabiiti

IGC Proceedings (1993-2023)

The cattle population of Uganda, estimated at 6 million, consists of more than 95% indigenous stock, raised on the semi-arid and semi-humid grasslands that make up 48% of the total land area and which supply over 85% of the marketed milk and meat. Native grassland pastures, which vary seasonal in quantity and quality due to rainfall and temperature variations, are the sole feed resource for cattle (Mbuza et al., 1992). This study examined the effects of seasonal herbage mass (HM), dietary crude protein (CPd), detergent lignin (ADLd) and digestibility (DiG) on body condition scores of …

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The Seasonal Dry Matter Production And Carrying Capacity Of Kikuyu Oversown With Ryegrass And Clover, Philip R. Botha, Robin Meeske, Hennie A. Snyman Apr 2023

The Seasonal Dry Matter Production And Carrying Capacity Of Kikuyu Oversown With Ryegrass And Clover, Philip R. Botha, Robin Meeske, Hennie A. Snyman

IGC Proceedings (1993-2023)

In the main milk producing areas of the Southern Cape, kikuyu (Pennisetum clandestinum) is considered an important summer and autumn pasture which is climatologically well adapted. The main problem experienced with kikuyu is that winter and spring production is low and the exclusion of legumes make it dependent of nitrogen fertiliser and that increases the input cost. The aim of the study was to quantify the seasonal dry matter (DM) yield and carrying capacity of kikuyu (K) through treatments involving kikuyu oversown with annual ryegrass (Lolium multiflorum spp.) (KR), kikuyu oversown with a mixture of perennial ryegrass …

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The Seasonal Botanical Composition, Calcium And Phosphorus Content Of Kikuyu Oversown With Ryegrass And Clover, Philip R. Botha, Robin Meeske, Hennie A. Snyman Apr 2023

The Seasonal Botanical Composition, Calcium And Phosphorus Content Of Kikuyu Oversown With Ryegrass And Clover, Philip R. Botha, Robin Meeske, Hennie A. Snyman

IGC Proceedings (1993-2023)

Kikuyu (Pennisetum clandestinum) is one of the major grasses used for summer and autumn grazing in the Southern Cape coast area of South Africa. Annual ryegrass (Lolium multiflorum spp.), perennial white (Trifolium repens) and red clover (Trifolium pratense) can be incorporated into an existing kikuyu stand to improve pasture quality and spring production. The aim of this study was to determine the persistence of these species and effects on the calcium and phosphorus content of kikuyu (K), kikuyu oversown with annual ryegrass (KR), kikuyu oversown with a mixture of perennial ryegrass (L. …

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Varietal Differences In Perennial Ryegrass For Fructan Metabolism And Their Relationship To Grazing Tolerance, B. Lasseur, J. Lothier, A. Morvan-Bertrand, M. O. Humphreys, M. P. Prud'homme Apr 2023

Varietal Differences In Perennial Ryegrass For Fructan Metabolism And Their Relationship To Grazing Tolerance, B. Lasseur, J. Lothier, A. Morvan-Bertrand, M. O. Humphreys, M. P. Prud'homme

IGC Proceedings (1993-2023)

Perennial ryegrass (Lolium perenne L.) is the most important grass in Europe; it often is defoliated. The link between fructan metabolism and defoliation tolerance has been studied in 2 Lolium perenne varieties, Aurora (high sugar perennial) and Perma (low-to-normal sugar perennial) (Turner et al., 2002).

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Variability In Quantity And Composition Of Water Soluble Carbohydrates Among Irish Accessions And European Varieties Of Perennial Ryegrass, S. Mcgrath, S. Barth, A. Frohlich, M. Francioso, S. A. Lamorte, T. R. Hodkinson Apr 2023

Variability In Quantity And Composition Of Water Soluble Carbohydrates Among Irish Accessions And European Varieties Of Perennial Ryegrass, S. Mcgrath, S. Barth, A. Frohlich, M. Francioso, S. A. Lamorte, T. R. Hodkinson

IGC Proceedings (1993-2023)

The objective of this study was to identify perennial ryegrass accessions displaying high fructose and glucose contents and an improved ratio between fructose and glucose fractions across different time points throughout the year. Fructose and glucose are the main constituents of the water soluble carbohydrate (WSC) fraction in perennial ryegrass. For animal nutrition the amount of WSC is crucial as it is the primary energy source available to metabolise the intake of plant protein. The ratio between fructose and glucose fractions is important since fructosan chains, which are an excellent energy source for ruminants, are built from fructose. Furthermore the …

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Breeding Of Cms-F1-Hybrids In Lolium Perenne With Improved Nitrogen Use Efficiency, W. Luesink, B. Ingwersen, L. Wolters, J. De Riek Apr 2023

Breeding Of Cms-F1-Hybrids In Lolium Perenne With Improved Nitrogen Use Efficiency, W. Luesink, B. Ingwersen, L. Wolters, J. De Riek

IGC Proceedings (1993-2023)

The environmental pollution by nitrogen losses from dairy farms can be reduced by improving the nitrogen use efficiency (NUE) of grass varieties. The main goal is to develop varieties with a better nitrogen utilisation. These low input varieties can produce acceptable yields at a low level of N-fertilisation. High input varieties express their high yield potential only at high N-supply. These varieties are less preferable, because N- losses increase at higher levels of nitrogen application. The breeding of CMS-F1-Hybrids can be a successful strategy for developing varieties with a higher NUE. In F1-Hybrid varieties higher heterosis effects can be achieved …

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A Survey Of European Regional Adaptation In Italian Ryegrass Varieties, T. J. Gilliland, A. J. P. Van Wijk Apr 2023

A Survey Of European Regional Adaptation In Italian Ryegrass Varieties, T. J. Gilliland, A. J. P. Van Wijk

IGC Proceedings (1993-2023)

Ryegrass is widely adapted to cool temperate eco-zones and breeders often submit individual varieties for testing in a number of EU countries. National testing programmes often combine data from several trial sites that may differ climatically, but not from sites in other member states, despite the possibility of high ecological similarity. Given increasing interest in animal value characters (soluble sugars, lipids, sward geometry), additional testing for these would be valuable but is prohibited by capped or declining funding. Data sharing between EU national authorities could be advantageous but is inhibited by the lack of statistically valid data on the sensitivity …

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Milk Production Potential Of Different Dairy Pasture Types In Southern Australia, J. Tharmaraj, D. F. Chapman, Z. N. Nie, A. P. Lane Apr 2023

Milk Production Potential Of Different Dairy Pasture Types In Southern Australia, J. Tharmaraj, D. F. Chapman, Z. N. Nie, A. P. Lane

IGC Proceedings (1993-2023)

The growth rate of traditional perennial ryegrass-based pastures commonly fails to meet herd feed requirements through winter and summer in non-irrigated dairy systems in southern Australia. Alternative pasture species can improve seasonal feed supply in this region (Tharmaraj & Chapman, 2005). However, the feeding value and milk production of these pastures must at least match perennial ryegrass if they are to be adopted successfully on dairy farms. This paper reports results of a comparison of the milk production potential of pasture types similar to those investigated by Tharmaraj & Chapman (2005) for their agronomic performance.

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Effect Of Strain Of Holstein-Friesian Cow And Feed System On Reproductive Performance In Seasonal-Calving Milk Production Systems Over Four Years, B. Horan, J. F. Mee, Myles Rath, P. O'Connor, P. Dillon Apr 2023

Effect Of Strain Of Holstein-Friesian Cow And Feed System On Reproductive Performance In Seasonal-Calving Milk Production Systems Over Four Years, B. Horan, J. F. Mee, Myles Rath, P. O'Connor, P. Dillon

IGC Proceedings (1993-2023)

In Ireland most dairy farms operate seasonal calving grass-based milk production systems. Feed demand and supply are matched by having calving highly concentrated in spring. This requires high pregnancy rates within a short time following the start of mating in late April or early May, but has become increasingly difficult to achieve due to declining fertility in Irish dairy herds (Mee, 2004). In New Zealand, cows of North American Holstein-Friesian origin have poorer fertility than New Zealand Holstein-Friesians on pasture-based seasonal calving systems (Harris & Kolver, 2001). The present study sought to determine the effect of strain of Holstein-Friesian (HF) …

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Phenotype X Herbage Allowance Interactions In Reproduction Of First Calf Heifers Grazing Semiarid Rangeland, J. W. Holloway, B. G. Warrington, M. K. Owens Apr 2023

Phenotype X Herbage Allowance Interactions In Reproduction Of First Calf Heifers Grazing Semiarid Rangeland, J. W. Holloway, B. G. Warrington, M. K. Owens

IGC Proceedings (1993-2023)

Cattle are differentially adapted to nutritional environments. The most sensitive measure of adaptation is reproduction of first-calf heifers. We studied the role of maturation rate and milk production on reproductive performance of first-calf heifers allowed different levels of herbage in semiarid rangeland.

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The Impact Of The Level Of Feed-On-Offer Available To Merino Ewes During Winter-Spring On The Wool Production Of Their Progeny As Adults, Chris Oldham, M. Ferguson, B. Paganoni, A. Thompson, G. Kearney, M. A. Van Burgel Apr 2023

The Impact Of The Level Of Feed-On-Offer Available To Merino Ewes During Winter-Spring On The Wool Production Of Their Progeny As Adults, Chris Oldham, M. Ferguson, B. Paganoni, A. Thompson, G. Kearney, M. A. Van Burgel

IGC Proceedings (1993-2023)

New opportunities for developing optimum ewe management systems, based on achieving liveweight and body condition score (CS) targets at critical stages of the reproductive cycle, have emerged from the acceptance that nutrition during pregnancy can have substantial impacts on the lifetime wool performance of the progeny (Kelly et al,. 1996). However, most studies of the impacts of nutrition on foetal growth and development tended to focus on late pregnancy and have also only considered extreme nutritional regimes often outside the boundaries of commercial reality. Hence, the Lifetime Wool team (Thompson & Oldham, 2004) conducted dose-response experiments to determine the …

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Effects Of Forage Chicory (Cichorium Intybus) On Farmed Deer Growth And Internal Parasitism, S. O. Hoskin, P. R. Wilson, W. E. Pomroy, T. N. Barry Apr 2023

Effects Of Forage Chicory (Cichorium Intybus) On Farmed Deer Growth And Internal Parasitism, S. O. Hoskin, P. R. Wilson, W. E. Pomroy, T. N. Barry

IGC Proceedings (1993-2023)

Internal parasitism (particularly lungworm - Dictyocaulus sp) significantly limits post-weaning growth of deer. Endoparasite control using anthelmintics may be unsustainable, due to the increasing risk of anthelmintic resistance and the risk or perception of chemical residues in animal products. Chicory has a high feeding value and contains sesquiterpene lactones and low levels of condensed tannins, both with anti-parasitic activity (Molan et al., 2003). Grazing chicory during autumn may reduce the requirement for anthelmintic treatment of young deer compared with grazing ryegrass-based pasture (Hoskin et al., 1999). The objective of this study was to investigate the effect of withholding …

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Gene Discovery And Molecular Dissection Of Lignin Biosynthesis In Perennial Ryegrass (Lolium Perenne), A. Lidgett, M. Emmerling, R. Heath, R. Mcinnes, D. Lynch, A. Bartkowski, K. Fulgueras, T. Sawbridge, E. K. Ong, K. F. Smith, A. Mouradov, G. C. Spangenberg Apr 2023

Gene Discovery And Molecular Dissection Of Lignin Biosynthesis In Perennial Ryegrass (Lolium Perenne), A. Lidgett, M. Emmerling, R. Heath, R. Mcinnes, D. Lynch, A. Bartkowski, K. Fulgueras, T. Sawbridge, E. K. Ong, K. F. Smith, A. Mouradov, G. C. Spangenberg

IGC Proceedings (1993-2023)

Lignification of plant cell walls has been identified as a major factor limiting forage digestibility. It limits the amount of digestible energy available to livestock, resulting in an incomplete utilisation of cellulose and hemicellulose by ruminant animals. Modification of the lignin profile of ryegrasses (Lolium spp.) and fescues (Festuca spp.) is undertaken through modulating the expression of genes encoding enzymes involved in the biosynthesis of monolignols.

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Identification Of Putative AtTt2 R2r3-Myb Transcription Factor Orthologues In Tanniferous Tissues Of L. Corniculatus Var. Japonicus Cv Gifu, D. N. Bryant, P. Bailey, P. Morris, Mark P. Robbins, C. Martin, T. Wang Apr 2023

Identification Of Putative AtTt2 R2r3-Myb Transcription Factor Orthologues In Tanniferous Tissues Of L. Corniculatus Var. Japonicus Cv Gifu, D. N. Bryant, P. Bailey, P. Morris, Mark P. Robbins, C. Martin, T. Wang

IGC Proceedings (1993-2023)

R2R3-MYB plant transcription factors are sequence–specific DNA-binding proteins, which regulate the expression of specific gene(s) following the R2R3 DNA-binding domain interacting with the corresponding promoter sequence(s). The biosynthetic pathway leading to the production of anthocyanins has been demonstrated to be under MYB transcriptional regulatory control (Cone et al., 1986), while the accumulation of proanthocyanidins (PAs) in Arabidopsis seed coats is determined by the R2R3-MYB AtTT2 (Nesi et al., 2001). Using an informatics approach, partial sequences of putative AtTT2 orthologues have been identified and cloned from the forage legume Lotus corniculatus var. japonicus cv Gifu.

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Foliar Expression Of Candidate Genes Involved In Condensed Tannin Biosynthesis In White Clover (Trifolium Repens), Stephen N. Panter, J. Simmonds, A. Winkworth, A. Mouradov, G. C. Spangenberg Mar 2023

Foliar Expression Of Candidate Genes Involved In Condensed Tannin Biosynthesis In White Clover (Trifolium Repens), Stephen N. Panter, J. Simmonds, A. Winkworth, A. Mouradov, G. C. Spangenberg

IGC Proceedings (1993-2023)

Bloat disease in cattle and sheep is caused by the rapid microbial degradation of protein-rich fodder in the rumen. This leads to the production of protein foams that trap gases, causing bloat, a condition that is often fatal to livestock and costly to farmers. Condensed tannins (CTs) are phenolic polymers produced by the phenylpropanoid pathway of plants (Figure 1). CTs bind to proteins under acidic to neutral conditions, such as those present in the rumen, slowing their breakdown. A diet with a CT content of between 2% and 4% by dry weight, which is provided by some pasture legumes (e.g. …

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Discovery, Isolation And Characterisation Of Promoters In White Clover (Trifolium Repens), C. M. Labandera, Y. H. Lin, E. Ludlow, M. Emmerling, U. John, P. W. Sale, C. Pallaghy, G. C. Spangenberg Mar 2023

Discovery, Isolation And Characterisation Of Promoters In White Clover (Trifolium Repens), C. M. Labandera, Y. H. Lin, E. Ludlow, M. Emmerling, U. John, P. W. Sale, C. Pallaghy, G. C. Spangenberg

IGC Proceedings (1993-2023)

The availability of a suite of promoters with a range of spatial, temporal and inducible expression patterns is of significant importance to control targeted expression of genes for molecular breeding in forage species. A range of resources and tools have been developed for promoter isolation and characterisation in white clover (Trifolium repens L.), including a comprehensive BAC library and a 15K unigene microarray.

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Novel Genotypes Of The Subtropical Grass Eragrostis Curvula For The Analysis Of Apomixis (Diplospory), S. Cardone, P. Polci, J. P. Selva, M. Mecchia, S. C. Pessino, P. Voigt, G. C. Spangenberg, V. Echenique Mar 2023

Novel Genotypes Of The Subtropical Grass Eragrostis Curvula For The Analysis Of Apomixis (Diplospory), S. Cardone, P. Polci, J. P. Selva, M. Mecchia, S. C. Pessino, P. Voigt, G. C. Spangenberg, V. Echenique

IGC Proceedings (1993-2023)

Eragrostis curvula (Schrad.) Nees is a variable grass native to Southern Africa. Its several forms, known as lovegrasses, were introduced to Australia, USA and Argentina as forage perennial grasses. Apomixis is a common trait in the genus Eragrostis, with diplospory being the most frequent type. Sexual reproduction also occurs in Eragrostis, although not frequently. Since most tetraploid Eragrostis lines are apomictic, the generation of a sexual tetraploid strain is a requirement for linkage analysis of the gene(s) governing the apomictic character. Furthermore, isogenic lines of the same ploidy, reproducing alternatively by sexuality or apomixes, represent an ideal system …

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Isolation And Characterisation Of Genes Encoding Malate Synthesis And Transport Determinants In The Aluminum-Tolerant Australian Weeping-Grass (Microlaena Stipoides), R. M. Polotnianka, E. Ribarev, L. Mackin, K. A. Sivakumaran, G. D. Nugent, U. P. John, G. C. Spangenberg Mar 2023

Isolation And Characterisation Of Genes Encoding Malate Synthesis And Transport Determinants In The Aluminum-Tolerant Australian Weeping-Grass (Microlaena Stipoides), R. M. Polotnianka, E. Ribarev, L. Mackin, K. A. Sivakumaran, G. D. Nugent, U. P. John, G. C. Spangenberg

IGC Proceedings (1993-2023)

Acid soils cover some 40% of the Earth’s arable land where they represent a major limitation to plant production. Plant growth on acid soils is primarily limited due to aluminium (Al) solubilized by acidity into toxic Al3+ cations which will inhibit root growth resulting in poor uptake of water and nutrients. Many important pasture species lack sufficient Al tolerance within their germplasm to allow effective breeding for this character.

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Development And Use Of A Tool For Automated Alignments Of Genes In The Rice Bac's Genbank Card Against Other Species, P. Barre, G. Darrieutort, J. Auzanneau, B. Julier Mar 2023

Development And Use Of A Tool For Automated Alignments Of Genes In The Rice Bac's Genbank Card Against Other Species, P. Barre, G. Darrieutort, J. Auzanneau, B. Julier

IGC Proceedings (1993-2023)

In many cases, the analysis of the genetic bases of any trait requires molecular markers and if possible co-dominant PCR-based ones. In perennial fodder species, the number of publicly available markers (microsatellites and Sequence Tagged Site (STS)) is limited. Our goal is to use sequences from model grass species, i.e. rice, wheat, maize, barley, in L. perenne in order to develop STS markers in interesting regions such as under a QTL (Quantitative Trait Loci) or around a candidate gene,. As the genome sequence of rice is now available, the objective was to use the sequences of genes included in the …

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Screening Of Perennial Grasses And A Mutant Maize Collection By Fourier-Transformed Infrared (Ftir) Spectroscopy For Improved Biofuel Traits, S. C. Thain, P. Morris, S. Hawkins, C. Morris, I. S. Donnison Mar 2023

Screening Of Perennial Grasses And A Mutant Maize Collection By Fourier-Transformed Infrared (Ftir) Spectroscopy For Improved Biofuel Traits, S. C. Thain, P. Morris, S. Hawkins, C. Morris, I. S. Donnison

IGC Proceedings (1993-2023)

Currently the potential of biomass crops, including grasses, is limited because most species have not been bred for this purpose. However traits such as lignification, phenolic cross-linking and carbohydrate accessibility, which are also important for nutritive quality in forage grasses, can affect potential biofuel quality in applications such as combustion, fast-pyrolysis or fermentation. A collection of Lolium and Festuca species known to exhibit a range of lignin, cell wall phenolic and carbohydrate concentrations have been used to test optimum characteristics for biofuel processing. This collection formed a “calibration” set for subsequent high through-put FTIR chemical screening of additional plant lines: …

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Application Of Molecular Markers Derived From Medicago Truncatula In White Clover (Trifolium Repens L.), C. Jones, M. T. Abberton Mar 2023

Application Of Molecular Markers Derived From Medicago Truncatula In White Clover (Trifolium Repens L.), C. Jones, M. T. Abberton

IGC Proceedings (1993-2023)

White clover is the major forage legume of temperate areas. Genome maps have been produced recently (Jones et al., 2003; Barrett et al., 2004) and the location of QTL for important agricultural traits reported (Abberton et al., 2004). White clover is closely related to the model legume Medicago truncatula and there is likely to be considerable benefit in applying genomic resources from model to crop. However, the extent of synteny between the species must be established. Here we present preliminary results detailing progress towards this goal.

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A Proposal For An International Transcriptome Initiative For Forage And Turf: Microarray Tools For Expression Profiling In Ryegrass, Clover And Grass Endophytes, T. Webster, N. Nguyen, C. Rhodes, S. A. Felitti, R. Chapman, D. Edwards, G. C. Spangenberg Mar 2023

A Proposal For An International Transcriptome Initiative For Forage And Turf: Microarray Tools For Expression Profiling In Ryegrass, Clover And Grass Endophytes, T. Webster, N. Nguyen, C. Rhodes, S. A. Felitti, R. Chapman, D. Edwards, G. C. Spangenberg

IGC Proceedings (1993-2023)

Knowledge of the expression pattern of genes provides a valuable insight into gene function and role in determining the observed heritable phenotype. High–density cDNA and oligonucleotide microarrays represent powerful tools for transcriptome analysis to gain an understanding of gene expression patterns for thousands of genes. Internationally coordinated efforts in transcriptome analyses and sharing of microarray resources will benefit the advancement of our understanding of gene function in forage and turf species.

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Screening Genes For Association With Loci For Nitrogen-Use Efficiency In Perennial Ryegrass By PyrosequencingTm, O. Dolstra, D. Dees, J. -D. Driesprong, E. N. Van Loo Mar 2023

Screening Genes For Association With Loci For Nitrogen-Use Efficiency In Perennial Ryegrass By PyrosequencingTm, O. Dolstra, D. Dees, J. -D. Driesprong, E. N. Van Loo

IGC Proceedings (1993-2023)

The application of marker-assisted selection to improve quantitative traits in perennial ryegrass (Lolium perenne) is cumbersome. It requires a priori knowledge on the association of markers and genes. The knowledge on the chromosomal location of major genes for quantitative traits as well as on gene sequences is rapidly growing. However, determination of the genetic constitution of parents prior to their use in breeding still is impractical. More realistic is to collect association data along with the testing activities needed for breeding new varieties. This study uses changes in allele frequency due to selection as a criterion for gene-trait …

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Gene-Associated Single Nucleotide Polymorphism (Snp) Discovery In Perennial Ryegrass (Lolium Perenne L.), J. W. Forster, N. O. I. Cogan, A. C. Vecchies, R. C. Ponting, M. C. Drayton, J. George, J. L. Dumsday, G. C. Spangenberg, K. F. Smith Mar 2023

Gene-Associated Single Nucleotide Polymorphism (Snp) Discovery In Perennial Ryegrass (Lolium Perenne L.), J. W. Forster, N. O. I. Cogan, A. C. Vecchies, R. C. Ponting, M. C. Drayton, J. George, J. L. Dumsday, G. C. Spangenberg, K. F. Smith

IGC Proceedings (1993-2023)

Perennial ryegrass (Lolium perenne L.) is the most important grass species for temperate pasture systems world-wide. Varietal improvement programs for this obligate outbreeding species are based on polycrossing of multiple parents to produce heterogeneous synthetic populations. The complexity of breeding systems creates challenges and opportunities for molecular marker technology development and implementation. Previous research has led to: the generation of a comprehensive suite of simple sequence repeat (SSR) markers, reference genetic map construction, comparative genetic studies, QTL identification, and population structure analysis. Emphasis has now shifted from the use of anonymous genetic markers linked to trait-specific genes to the …

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Isolation Of Candidate Genes Involved In Cold Temperatures Response In Festuca Pratensis Huds., Using Suppression Subtractive Hybridisation And Microarray Approaches, H. Rudi, V. Alm, L. Opseth, A. Larsen, O. A. Rognli Mar 2023

Isolation Of Candidate Genes Involved In Cold Temperatures Response In Festuca Pratensis Huds., Using Suppression Subtractive Hybridisation And Microarray Approaches, H. Rudi, V. Alm, L. Opseth, A. Larsen, O. A. Rognli

IGC Proceedings (1993-2023)

The objective of this work was to isolate candidate genes which are differentially expressed following cold-acclimation and develop SNPs to test for associations between candidate genes and frost tolerance. The ability to develop sufficient levels of tolerance against freezing temperatures through cold-acclimation (hardening) is crucial for survival of grasses and winter cereals in temperate climate. Meadow fescue (Festauca pratensis Huds.) is one of the most important forage grass species in Northern Europe. The preference of Festuca instead of Lolium in Norway is due to its superior combination of winter hardiness and forage quality.

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Positive Effect Of Increased Aflp Diversity Among Parental Plants On Yield Of Polycross Progenies In Perennial Ryegrass (Lolium Perenne L.), R. Kölliker, B. Boller, F. Widmer Mar 2023

Positive Effect Of Increased Aflp Diversity Among Parental Plants On Yield Of Polycross Progenies In Perennial Ryegrass (Lolium Perenne L.), R. Kölliker, B. Boller, F. Widmer

IGC Proceedings (1993-2023)

In outbreeding forage crops such as perennial ryegrass (Lolium perenne L.), genetic diversity among parental plants may greatly influence the success of a cultivar through mechanisms such as heterosis and inbreeding depression. The aim of this study was to evaluate the use of amplified fragment length polymorphism (AFLP) markers to select polycross parents with contrasting levels of genetic diversity and to analyse genetic diversity and agronomic performance of first and second generation synthetic progenies (Syn1, Syn2).

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