Physical Sciences and Mathematics Commons^™

Candida Albicans Hyphal Mannan Is Structurally Distinct From Yeast Mannan, Francis Kwofie

Electronic Theses and Dissertations

C. albicans is a polymorphic fungal pathogen which has the ability to shift from yeast to hyphae. C. albicans cell wall is composed of glucan, chitin, mannoprotein and mannan. It is not possible, using standard extraction methods, to isolate mannan from C. albicans hyphae. To isolate hyphal mannan, we developed a simplified alkali extraction method. Using this method it was determined that hyphal mannan has a much lower molecular weight, a smaller polymer distribution and altered conformation structure when compared to yeast mannan. The hyphal mannan was found to contain little to no acid-labile portion with only α-Man-PO₄ groups …

Combination Treatment With Conjugated Linoleic Acid And Nitrite Protects Against Myocaridal Infarction., Natia Qipshidze Kelm

Electronic Theses and Dissertations

According to the CDC, the most common type of heart disease is coronary artery disease, which frequently leads to myocardial infarction (MI). Therapeutic approaches to lessen the resulting cardiovascular injury associated with MI are limited. Recently, the management paradigm for cardiac injury has entered the molecular era and microRNAs (miRNAs) have been shown to act as negative regulators of gene expression by inhibiting mRNA translation and/or stimulating mRNA degradation. A single miRNA can modulate physiological or disease phenotypes by regulating whole functional systems. Importantly, miRNAs can regulate cardiac function, thereby modulating heart muscle contraction, heart growth and morphogenesis. MicroRNA-499 (miRNA-499) …

Optical And Sensing Properties Of Various Shaped Gold Nanoplates And Highly Controlled Asymmetric Gold Nanoplate/Nanosphere Coupled Assemblies., Aiqin Fang

Electronic Theses and Dissertations

With the development of a strategy to correlate the dark-field light scattering spectra of individual nanostructures with scanning electron microscopy (SEM) and atomic force microscopy (AFM) images of the same nanostructures, we were able to investigate several interesting optical properties of Au nanoplates (NPs) and asymmetrically-coupled Au nanospheres (NSs) attached to Au NPs with a high level of control. The light scattering spectra of the NP/NS coupled structures depend strongly on the location of NS attachment on the NP. Attachment of multiple NSs at the edge/vertex sites leads to a unique synergistic effect. In contrast to the uniform distribution of …

Parental Involvement, Students' Self-Engagement, And Academic Achievement: A Structural Equation Model, Cuirong Wu

Electronic Theses and Dissertations

In the ever-evolving landscape of China's education system, the gap between the rural and urban was always an important issue, not only the multifaceted interplay of parental involvement, student self-engagement, and academic performance. This study utilized a comprehensive national survey dataset through a thorough understanding of cultural nuances and educational intricacies specific to the Chinese context. Its aim was not only to decipher the underlying constructs of parental involvement and student self-engagement but also to investigate how these factors impacted academic achievement among students. The research unfolded in several stages using a representative sample of 10750 students from 112 schools …

Dihedral-Like Constructions Of Automorphic Loops, Mouna Ramadan Aboras

Electronic Theses and Dissertations

In this dissertation we study dihedral-like constructions of automorphic loops. Automorphic loops are loops in which all inner mappings are automorphisms. We start by describing a generalization of the dihedral construction for groups. Namely, if (G , +) is an abelian group, m > 1 and α ∈2 Aut(G ), let Dih(m, G, α) on Z_m × G be defined by

(i, u )(j, v ) = (i + j , ((-1)^j u + v )α^ij ).

We prove that the resulting loop is automorphic if and only if m = 2 …

Spectropolarimetry Of Epsilon Aurigae: Probing Stellar And Disk Atmospheres, Kathleen M. Geise

Electronic Theses and Dissertations

The bright eclipsing binary system epsilon Aurigae offers a unique opportunity to uncover physical mechanisms contributing to disk formation and evolution and to explore the relationship between photospheric anisotropies, stellar pulsation and mass loss. This research contributes to our understanding of stellar evolution in the context of binary stars. The research also offers the opportunity to investigate disk formation and evolution significant to our understanding of protoplanetary disks now seen in many star systems. Lastly, the project considers radiative transfer of polarized light that contributes to diverse fields such as atmospheric studies of exoplanets.

My objective is to understand the …

Novel Applications Of And Extensions To Linear Regression Methods For The Biomedical And Materials Sciences., Joe Bible

Electronic Theses and Dissertations

In this work we present three topics, each of which centered on either the application or modification of various linear regression methods. Our work with respect to the “Materials Genome” project while undermined by oversimplification and data integrity issues in its early stages, provides a sound platform from which the project can proceed successfully. Building upon a growing body of knowledge around the use of Weighted Generalized Estimating Equations (WGEE), our second investigation proposes an extension to that framework intended to address the inherent bias present in the analysis of clustered longitudinal data with potentially informative cluster sizes and temporal …

Sulfur-Oxidation Enhances Nitrile Hydration In Bioinspired Ruthenium Complexes : Catalytic, Kinetic, And Dft Investigations., Davinder Kumar

Electronic Theses and Dissertations

The current dissertation focuses on the effects of sulfur‒oxidation on nitrile hydration activity of a series of ruthenium(II) complexes inspired by nitrile hydratase (NHase). NHase catalytically hydrates nitriles to amides at a low spin non‒heme Fe(III) or non‒corrin Co(III) metal center. The active‒site employs a N2S3X type donor set using two carboxamido nitrogens and three cofacial cysteinic sulfurs that are present in distinct oxidation states as sulfinate (RSO2¯), sulfenate (RSO¯), and thiolate (RS¯). The apical site is occupied by substrate or solvent. A lack of sulfur‒oxidation renders the enzyme inactive. Three precatalysts, LnRuPPh3 (n = 1‒3), are employed to assess …

Distance-2 Domatic Numbers Of Graphs, Derek Kiser

Electronic Theses and Dissertations

The distance d(u, v) between two vertices u and v in a graph G equals the length of a shortest path from u to v. A set S of vertices is called a distance-2 dominating set if every vertex in V \S is within distance-2 of at least one vertex in S. The distance-2 domatic number is the maximum number of sets in a partition of the vertices of G into distance-2 dominating sets. We give bounds on the distance-2 domatic number of a graph and determine the distance-2 domatic number of selected classes of graphs.

Using Geometric Morphometrics To Differentiate Lower First Molars Of Microtus Species: A Review Of The Clark’S Cave Bone Deposit, Va, Mark Shelleman

Electronic Theses and Dissertations

Clark’s Cave contains a large collection of late Pleistocene mammal material. In particular, it contains a sizable amount of Microtus spp. which can be valuable paleoclimate indicators. Identification techniques traditionally used to classify these species have been shown to be unreliable. Recent studies have shown that using geometric morphometric techniques on lower first molars can be more successful. By placing landmarks and running a discriminate analysis on new and previously collected material from the cave, significant differences in Microtus species proportions were found. Specifically, showing the deposit has a larger proportion of M. xanthognathus than previously reported; resulting in a …

Ontogenesis In The Cranium Of Alligator Mississippiensis Based On Disarticulated Cranial Elements, William Henry Harris

Electronic Theses and Dissertations

The American alligator, Alligator mississippiensis, is a large extant archosaur and member of the Order Crocodilia. Crocodilian ontogeny has been studied in great detail, the skull being of particular interest. One aspect of the skull left unstudied is how individual cranial elements change through ontogeny independent of one another. This study observed morphological change in a growth series of 34 specimens of A. mississippiensis from ETSU Vertebrate Paleontology Lab collections. The premaxilla, maxilla, nasal, jugal, frontal, and parietal were analyzed using landmark morphometrics. The frontal, jugal, and parietal showed more allometric growth with the orbits reducing in size posteriorly. …

Discovering Rock Features With Geophysical Exploration And Archaeological Testing At The Mississippian Pile Mound Site, Upper Cumberland Plateau, Tennessee, Jeremy G. Menzer

Electronic Theses and Dissertations

The Pile Mound survey includes magnetometry paired with targeted ground-penetrating radar (GPR) and electromagnetic induction (EMI) surveys of the mound and testing of associated features over the ca. 6.5 ha site. The GPR survey discovered six rock features (five large rock features within the mound and one marking the outside of the mound). Knowledge of mounds in the Upper Cumberland Plateau (UCP) is lacking—the closest other studied sites are at the Corbin Site, Croley-Evans, Bell Site, and Beasley Mounds, approximately 75 – 100 km away. However, the most similar mound construction is found at Corbin and Cherokee sites, some 175 …

A Hierarchical Graph For Nucleotide Binding Domain 2, Samuel Kakraba

Electronic Theses and Dissertations

One of the most prevalent inherited diseases is cystic fibrosis. This disease is caused by a mutation in a membrane protein, the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is known to function as a chloride channel that regulates the viscosity of mucus that lines the ducts of a number of organs. Generally, most of the prevalent mutations of CFTR are located in one of two nucleotide binding domains, namely, the nucleotide binding domain 1 (NBD1). However, some mutations in nucleotide binding domain 2 (NBD2) can equally cause cystic fibrosis. In this work, a hierarchical graph is built for NBD2. …

Additional Research And Taxonomic Resolution Of Salamanders (Amphibia: Caudata) From The Mio-Pliocene Gray Fossil Site, Tn, Hannah E. Darcy

Electronic Theses and Dissertations

The Gray Fossil Site (GFS), a Mio-Pliocene (4.5–7 Ma) locality in the southern Appalachians, boasts the most diverse pre-Pleistocene salamander fauna in North America: Desmognathus sp., Plethodon sp., Notophthalmus sp., a Spelerpinae-type plethodontid, and Ambystoma sp. Because greater taxonomic resolution can result in more precise paleobiological interpretations, additional specimens were studied here. ETMNH 8045, a nearly complete articulated ambystomatid, appears most like Ambystoma maculatum in dentition and vertebral proportions. ETMNH 18219, an isolated vomer, is consistent with modern Pseudotriton and Gyrinophilus in possessing a postdentigerous process and a similar dentigerous row morphology. If these taxa, or species …

Effects Of Select Vitamin E Isoforms On The Production Of Polyunsaturated Fatty Acid Metabolites In Colorectal Cancer, Martha A. Borketey

Electronic Theses and Dissertations

Vitamin E exhibits anti-tumor activity by regulating pathways in cancer cells, potentially the lipoxygenase (LOX) pathway. We studied the effects of alpha tocopherol (AT), gamma tocopherol (GT), gamma tocotrienol (GT3), and an alpha-gamma tocopherol mixture (ATGT) on the production of the LOX metabolites 13-hydroxyoctadecaenoic acid (HODE), 15-hydroxyeicosatetraenoic acid (HETE), 12-HETE, and 5-HETE in colorectal cancer. These metabolites were examined in the HCT-116 cell line after 24 h treatment with select vitamin E isoforms and quantified by LC/MS/MS. Under physiological conditions, we find that treatment with varying vitamin E isoforms have different effects on the production of 13-HODE, 15-HETE, 12-HETE, and …

Synthesis And Characterization Of Five New Tetrakis(N-Phenylacetamidato) Dirhodium(Ii) Amine Complexes And One Molybdenum Cofactor Described Crystallographically, Cragin K. Harris

Electronic Theses and Dissertations

Six new crystal structures were determined using a Rigaku Mercurcy 375/MCCD(XtaLab mini) diffractometer. The structure of a molybdenum cofactor was solved resulting in an R1 (R1 = Σ ||Fo| - |Fc|| / Σ |Fo|) of 3.61% despite the presence of a disordered DMSO molecule. New Tetrakis(N-phenylacetamidato) Dirhodium(II) complexes were synthesized and characterized. Two 2,2-cis-[Rh2(NPhCOCH3)4]•(C3H4N2)x where x= 1 or 2 were successfully crystallized and solved with R1 values below 5%. Additional studies were conducted via NMR to observe formation of both products. Three potential catalysts were synthesized starting with 3,1-[Rh₂(NPhCOCH₃)₄]. The resulting compounds were a …

A Study Of The Catalytic And Chemical Properties Of Palladium And Palladium-Silver Monolayer Protected Clusters., Luther Benjamin Hutcherson

Electronic Theses and Dissertations

This thesis describes a study of the ligand chemistry of Pd Monolayer-Protected Clusters (MPCs) synthesized in the presence of octylamine. MPCs consists of a metal core surrounded by various organic stabilizers. Pd MPCs coated with alkanethiols, alkylamine, dendrimers, surfactants, and polymers have recently been explored for catalysis applications.Pd MPCs coated with alkylamines are more catalytically active than alkanethiols for hydrogentation/isomerization of allyl alcohol, but at shorter chain lengths are unstable in H2. We explored Pd MPCs synthesized in the presence of octylamines and discovered that the ligands attach to Pd as octanenitrile. This amine to nitrile conversion is catalyzed by …

Improved Self-Consistency For Sced-Lcao., Lyle C. Smith

Electronic Theses and Dissertations

In this document I describe a novel implementation of the generalized bisection method for finding roots of highly non-linear functions of several variables. Several techniques were optimized to reduce computation time. The implementation of the bisection method allows for the calculation of heterogeneous systems with SCED-LCAO, since derivative-based methods often fail for these systems. Systems composed of Gallium and Nitrogen are currently receiving much interest due to their behavior as semi-conductors and their ability to form nano-wires. The methods developed here were employed to create a set of SCED-LCAO parameters for homogeneous Gallium and heterogeneous Gallium Nitride systems. These parameters …

Exploring Ways Of Identifying Outliers In Spatial Point Patterns, Jie Liu

Electronic Theses and Dissertations

This work discusses alternative methods to detect outliers in spatial point patterns.

Outliers are defined based on location only and also with respect to associated variables. Throughout the thesis we discuss five case studies, three of them come from experiments with spiders and bees, and the other two are data from earthquakes in a certain region. One of the main conclusions is that when detecting outliers from the point of view of location we need to take into consideration both the degree of clustering of the events and the context of the study. When detecting outliers from the point of …

Using Kriging, Cokriging, And Gis To Visualize Fe And Mn In Groundwater, Crystal Johnson

Electronic Theses and Dissertations

For aesthetic, economic, and health-related reasons, allowable concentrations of iron (Fe) and manganese (Mn) found present in drinking water are 0.3 mg/L and 0.05 mg/L, respectively. Water samples taken from private drinking wells in the rural communities within Buncombe County, North Carolina contain amounts of these metals in concentrations higher than the suggested limits. This study focused on bedrock geology, elevation, saprolite thickness, and well depth to determine factors affecting Fe and Mn. Using ArcGIS 10.2, spatial trends in Fe and Mn concentrations ranges were visualized, and estimates of the metal concentrations were interpolated to unmonitored areas. Results from this …

Optimized Acid/Base Extraction And Structural Characterization Of Β-Glucan From Saccharomyces Cerevisiae, Shardrack O. Asare

Electronic Theses and Dissertations

β-glucan is a major component of the fungal cell wall consisting of (1→3)-β linked glucose polymers with (1→6)-β linked side chains. The published classical isolation procedure of β-glucan from Saccharomyces cerevisiae is expensive and time-consuming. Thus, the aim of this research was to develop an effective procedure for the extraction of glucans. We have developed a new method for glucan extraction that will be cost effective and will maintain the native structure of the glucan. The method that we developed is 80% faster and utilizes ¹/₃ of the reagents compared to the published classical method. Further, the method …

High-Precision Time-Series Photometry For The Discovery And Characterization Of Transiting Exoplanets., Karen Alicia Collins 1962-

Electronic Theses and Dissertations

The discovery of more than a thousand planets orbiting stars other than the Sun (i.e. exoplanets) over the past 20 years has shown that planetary systems are commonplace in the Milky Way galaxy. However, these discoveries are only a starting point in the quest to answer one of the most compelling questions posed by mankind for centuries – "are we alone in the universe?". This research aims to help build the foundation needed to search for that answer by optimizing data reduction techniques, determining and refining fundamental properties of known exoplanets, and searching for new exoplanets. Many exoplanets have been …

Optcluster : An R Package For Determining The Optimal Clustering Algorithm And Optimal Number Of Clusters., Michael N. Sekula

Electronic Theses and Dissertations

Determining the best clustering algorithm and ideal number of clusters for a particular dataset is a fundamental difficulty in unsupervised clustering analysis. In biological research, data generated from Next Generation Sequencing technology and microarray gene expression data are becoming more and more common, so new tools and resources are needed to group such high dimensional data using clustering analysis. Different clustering algorithms can group data very differently. Therefore, there is a need to determine the best groupings in a given dataset using the most suitable clustering algorithm for that data. This paper presents the R package optCluster as an efficient …

Summary Of Survival Analysis With Sas Procedures., Derek Duane Childers 1990-

Electronic Theses and Dissertations

The research conducted for this thesis was performed to summarize some of the most commonly used survival analysis techniques as well as to create one macro that will provide the solutions for these techniques. Some of the techniques that this thesis focuses on are survival and hazard functions, mean and median survival times, life table, log rank test, proportional hazards/model building, and competing risk. To further analyze these survival analysis techniques I will use the Bone Marrow Transplantation for Leukemia dataset. This trial consists of either acute myelocytic leukemia (AML 99 patients) or acute lymphoblastic leukemia (ALL 38 patients). There …

The Apprentices' Tower Of Hanoi, Cory Bh Ball

Electronic Theses and Dissertations

The Apprentices' Tower of Hanoi is introduced in this thesis. Several bounds are found in regards to optimal algorithms which solve the puzzle. Graph theoretic properties of the associated state graphs are explored. A brief summary of other Tower of Hanoi variants is also presented.

Revised Model For Antibiotic Resistance In A Hospital, Ruhang Pei

Electronic Theses and Dissertations

In this thesis we modify an existing model for the spread of resistant bacteria in a hospital. The existing model does not account for some of the trends seen in the data found in literature. The new model takes some of these trends into account. For the new model, we examine issues relating to identifiability, sensitivity analysis, parameter estimation, uncertainty analysis, and equilibrium stability.

Analysis Of Anacardic Acid On The Surface Of Pelargonium × Hortorum Using Matrix Assisted Laser Desorptoin Ionization Mass Spectrometry Imaging., Bryan Edward Wessel 1989-

Electronic Theses and Dissertations

The biosynthetic pathway leading to the production of anacardic acids (AnAcs) in glandular trichomes on the surface of geranium plants remains unclear. Anacardic acids are salicylic acid derivatives known to possess various medicinal benefits. Gene silencing techniques, such as RNA interference, could be useful in determining specific enzymes involved in the synthesis of AnAcs. However, it is first necessary to develop methodologies to image AnAcs. In this project, both fluorescence (FL) and mass spectral imaging (MSI) were tested. Our results indicate that cellular imaging of AnAcs, via FL, is not suitable because the spectral ranges of absorption and emission of …

Fail-Safe Test Generation Of Safety Critical Systems, Salwa Elakeili

Electronic Theses and Dissertations

This dissertation introduces a technique for testing proper failure mitigation in safety critical systems. Unlike other approaches which integrate behavioral and failure models, and then generate tests from the integrated model, we build safety mitigation tests from an existing behavioral test suite, using an explicit mitigation model for which we generate mitigation paths which are then woven at selected failure points into the original test suite to create failure-mitigation tests (safety mitigation test).

Improving Fairness, Throughput And Blocking Performance For Long Haul And Short Reach Optical Networks, Sana Tariq

Electronic Theses and Dissertations

Innovations in optical communication are expected to transform the landscape of global communications, internet and datacenter networks. This dissertation investigates several important issues in optical communication such as fairness, throughput, blocking probability and differentiated quality of service (QoS). Novel algorithms and new approaches have been presented to improve the performance of optical circuit switching (OCS) and optical burst switching (OBS) for long haul, and datacenter networks. Extensive simulations tests have been conducted to evaluate the effectiveness of the proposed algorithms. These simulation tests were performed over a number of network topologies such as ring, mesh and U.S. Long-Haul, some high …

Chemical Analysis, Databasing, And Statistical Analysis Of Smokeless Powders For Forensic Application, Dana-Marie Dennis

Electronic Theses and Dissertations

Smokeless powders are a set of energetic materials, known as low explosives, which are typically utilized for reloading ammunition. There are three types which differ in their primary energetic materials; where single base powders contain nitrocellulose as their primary energetic material, double and triple base powders contain nitroglycerin in addition to nitrocellulose, and triple base powders also contain nitroguanidine. Additional organic compounds, while not proprietary to specific manufacturers, are added to the powders in varied ratios during the manufacturing process to optimize the ballistic performance of the powders. The additional compounds function as stabilizers, plasticizers, flash suppressants, deterrents, and opacifiers. …