Physical Sciences and Mathematics Commons^™

Reconstitution Of A Minimal Dna Replicase From Pseudomonas Aeruginosa And Stimulation By Non-Cognate Auxiliary Factors, Thale C. Jarvis, Amber A. Beaudry, James M. Bullard, Nebojsa Janjic, Charles S. Mchenry

Chemistry Faculty Publications and Presentations

DNA polymerase III holoenzyme is responsible for chromosomal replication in bacteria. The components and functions of Escherichia coli DNA polymerase III holoenzyme have been studied extensively. Here, we report the reconstitution of replicase activity by essential components of DNA polymerase holoenzyme from the pathogen Pseudomonas aeruginosa. We have expressed and purified the processivity factor (β), single-stranded DNA-binding protein, a complex containing the polymerase (α) and exonuclease (ϵ) subunits, and the essential components of the DnaX complex (τ₃δδ′). Efficient primer elongation requires the presence of αϵ, β, and τ₃δδ′. Pseudomonas aeruginosa αϵ can substitute completely for …

Thor: The Hybrid Online Repository, Timothy W. Van Der Horst

Theses and Dissertations

Digital credentials enable users to perform secure interactions by proving either their identity or that they posses certain attributes. Special care is taken to protect these credentials and their associated private keys during transaction time. However, protection of these items outside of the transaction is often delegated to a secure credential repository. A mobile environment creates significant challenges for secure repositories. We examine these challenges with respect to existing repository practices and produce a set of requirements that a repository must meet in order to cope with the harshness of a mobile environment. We also present the design and implementation …

The Virginia Wetlands Report Vol. 20, No. 1, Virginia Institute Of Marine Science

Virginia Wetlands Reports

• Clapper Rail (Rallus longirostris). Julie Bradshaw
• Tidal Wetlands Seminar Draws Crowd
• Annual Summary of Permitted Tidal Wetland Impacts - 2004. Karen Duhring
• Take a Wetland to Lunch…. Or Take your Lunch to a Wetland Pam Mason
• A Level I Protocol for Assessing Wetland Condition by Hydrologic Unit within the Coastal Plain. Kirk Havens

The Impact Of California's Changing Environmental Regulations On Timber Harvest Planning Costs, Richard P. Thompson, Christopher Dicus

Natural Resources Management and Environmental Sciences

The primary purpose of this study is to establish basic and supportable information on the impact of environmental regulations on California’s forest products industry. More specifically, the study focused on the effects of changing forest practice regulations on timber harvest planning and preparation costs. A survey of wood-processing and forestry consulting firms was conducted in the Summer and early Fall, 2004 seeking data on Timber Harvest Plan (THP) preparation costs, a major component of the transactions cost in California’s timber market. Despite the short data collection period, 607 sample observations were obtained. Analysis of the sample data clearly indicate significant …

Adaptive Decision Support For Planning Under Hard And Soft Constraints, Alex Dekhtyar, Raphael Finkel, Judy Goldsmith, Beth Goldstein, Cynthia Isenhour

Computer Science and Software Engineering

We describe the “Welfare to Work” scenario, and the software we are designing to support case managers’ planning for their clients.

Coral Reef Electronic Chart Initiative, Protecting Corals, Saving Ships, Lee Alexander, Kathryn L. Ries

Center for Coastal and Ocean Mapping

The Office of Coast Survey, NOAA is conducting a pilot project in the Florida Keys National Marine Sanctuary to convert existing coral, marine protected areas (MPA) and other marine GIS information into a format suitable for use with shipboard Electronic Chart Display and Information Systems (ECDIS). Specifically, existing data will be converted into Marine Information Objects (MIOs) conforming to IHO S-57 data standards that become a supplemental information layer to be used with Electronic Navigational Charts (ENC) in ECDIS. The project goal is to strengthen marine resource conservation by bringing critical coral, MPA, and other environmental protection-related information to the …

The Norm Of A Composition Operator With Linear Symbol Acting On The Dirichlet Space, Christopher Hammond

Mathematics Faculty Publications

We obtain a representation for the norm of a composition operator on the Dirichlet space induced by a map of the form φ(z)=az+b. We compare this result to an upper bound for ‖Cφ‖ that is valid whenever φ is univalent. Our work relies heavily on an adjoint formula recently discovered by Gallardo-Gutiérrez and Montes-Rodríguez.

Who Counts Your Votes?, Halina Kaminski, Lila Kari, Mark Perry

Computer Science Publications

Open and fair elections are paramount to modern democracy. Although some people claim that the penciland- paper systems used in countries such as Canada and UK are still the best method of avoiding vote rigging, recent election problems have sparked great interest in managing the election process through the use of electronic voting systems. It is a goal of this paper to describe a voting system that is secret and secure as well as verifiable and useable over an existing computer network. We have designed and implemented an electronic voting system – Verifiable E-Voting (VEV) – with an underlying protocol …

Wright State University College Of Engineering And Computer Science Bits And Pcs Newsletter, Volume 21, Number 6, March 2005, College Of Engineering And Computer Science, Wright State University

BITs and PCs Newsletter

An eight page newsletter created by the Wright State University College of Engineering and Computer Science that addresses the current affairs of the college.

What Does Height Really Mean? Part Ii: Physics And Gravity, Thomas H. Meyer, Daniel R. Roman, David B. Zilkoski

Thomas H. Meyer's Peer-reviewed Articles

This is the second paper in a four-part series considering the fundamental question, “what does the word height really mean?” The first paper in this series explained that a change in National Geodetic Survey’s policy, coupled with the modern realities of GPS surveying, have essentially forced practicing surveyors to come to grips with the myriad of height definitions that previously were the sole concern of geodesists. The distinctions between local and equipotential ellipsoids were considered, along with an introduction to mean sea level. This paper brings these ideas forward by explaining mean sea level and, more importantly, the geoid. The …

A Sustainable Future For Recreational Fishing In The Cocos (Keeling) Islands. Proposal For Discussion., Department Of Fisheries.

Fisheries management papers

Fishing activity on the Cocos is essentially unmanaged at present and there has been some condern by the local community that a number of species, such as coral trout, have declined in numbers over the last decade. This draft strategy was developed by the Western Australian Department of Fisheries who now has responsibility for managing recreational fishing on behalf of the Commonwealth Department of Transport and Regional Services.

Acute Toxicity Testing Without Animals: More Scientific And Less Of A Gamble, Gillian R. Langley

Application of Alternative Methods Collection

In this report, we argue specifically that acute toxicity data should not be sought from animal tests. The underlying principle of such tests on rats and mice is that the results can be effectively extrapolated to humans. In fact, after nearly 80 years of use of these tests, the predictivity of rodent data for human acute toxic effects has been disputed but never proven.

Evaluation Of Attractants For Live-Trapping Nine-Banded Armadillos, Daniel J. Gammons, Michael T. Mengak, L. Mike Conner

Other Publications in Wildlife Management

In the past 50 years, the range of the nine-banded armadillo (Dasypus novemcinctus) in the south has been rapidly expanding. As their range expands, armadillos increasingly come into conflict with suburban landowners. When foraging, armadillos often uproot ornamental plants. Their rooting also destroys gardens, lawns, and flower beds. Their burrowing can damage tree roots and building foundations. Most armadillo damage is a result of their feeding habits. Armadillos dig shallow holes, 1-3 inches deep and 3-5 inches long, as they search for soil invertebrates. A recent survey of Georgia county extension agents by scientists at the University of …

Serum Proteomics Profiling: A Young Technology Begins To Mature, Kevin R. Coombes, Jeffrey S. Morris, Jianhua Hu, Sarah R. Edmondson, Keith A. Baggerly

Jeffrey S. Morris

No abstract provided.

A Statistical Framework For The Analysis Of Microarray Probe-Level Data, Zhijin Wu, Rafael A. Irizarry

Johns Hopkins University, Dept. of Biostatistics Working Papers

Microarrays are an example of the powerful high through-put genomics tools that are revolutionizing the measurement of biological systems. In this and other technologies, a number of critical steps are required to convert the raw measures into the data relied upon by biologists and clinicians. These data manipulations, referred to as preprocessing, have enormous influence on the quality of the ultimate measurements and studies that rely upon them. Many researchers have previously demonstrated that the use of modern statistical methodology can substantially improve accuracy and precision of gene expression measurements, relative to ad-hoc procedures introduced by designers and manufacturers of …

Size-Driven Domain Reorientation In Hydrothermally Derived Lead Titanate Nanoparticles, Zhiyuan Ye, Elliot B. Slamovich, Alexander H. King

Alexander H. King

High-resolution transmission electron microscopy studies of hydrothermally derived platelike lead titanate nanoparticles reveal that below a critical size of approximately 70 nm, the single ferroelectric domain polarization axis reorients from perpendicular to parallel to the plate. We suggest that during particle growth, ions in the hydrothermal processing medium compensate for the ferroelectric depolarization energy. When the processing medium is removed by washing and drying, single domain nanoparticles minimize their depolarization energy by c-axis flipping.

The Connections Between Qso Absorption Systems And Galaxies: Low-Redshift Observations, Todd M. Tripp, David V. Bowen

Todd M. Tripp

Quasar absorption lines have long been recognised to be a sensitive probe of the abundances, physical conditions, and kinematics of gas in a wide variety of environments including low-density intergalactic regions that probably cannot be studied by any other means. While some pre-Hubble Space Telescope (HST) observations indicated that Mg II absorption lines arise in gaseous galactic halos with a large covering factor, many early QSO absorber studies were hampered by a lack of information about the context of the absorbers and their connections with galaxies. By providing access to crucial ultraviolet resonance lines at low redshifts, deployment of HST …

Low Regularity Solutions To A Gently Stochastic Nonlinear Wave Equation In Nonequilibrium Statistical Mechanics, L Rey-Bellet, Le Thomas

Luc Rey-Bellet

We consider a system of stochastic partial differential equations modeling heat conduction in a non-linear medium. We show global existence of solutions for the system in Sobolev spaces of low regularity, including spaces with norm beneath the energy norm. For the special case of thermal equilibrium, we also show the existence of an invariant measure (Gibbs state).

Cesu Agreements: Beyond Traditional Science Applications, Margaret N. Rees

Presentations (PLI)

Partnering with four federal land-management agencies

• Bureau of Land Management
• National Park Service
• U.S. Forest Service
• U.S. Fish & Wildlife Service

Relative Equilibria For The Generalized Rigid Body, Antonio Hernandez-Garduno, Jeffrey K. Lawson, Jerrold E. Marsden

Mathematics Faculty Research

This paper gives necessary and sufficient conditions for the (n-dimensional) generalized free rigid body to be in a state of relative equilibrium. The conditions generalize those for the case of the three-dimensional free rigid body, namely that the body is in relative equilibrium if and only if its angular velocity and angular momentum align, that is, if the body rotates about one of its principal axes. For the n-dimensional rigid body in the Manakov formulation, these conditions have a similar interpretation. We use this result to state and prove a generalized Saari’s Conjecture (usually stated for the …

Prevailing Weather Conditions During Summer Seasons Around Gangotri Glacier, Pratap Singh, Umesh K. Haritashya, K. S. Ramasastri, Naresh Kumar

Geology Faculty Publications

Meteorological data collected near the snout of the Gangotri Glacier suggest that the study area receives less rainfall. The average seasonal rainfall is observed to be about 260 mm. The rainfall distribution does not show any monsoon impact. Amount of seasonal rainfall is highly variable (131.4-368.8 mm) from year to year, but, in general, August had the maximum rainfall. A verage daily maximum and minimum temperatures were 14.7 and 4.1°C respectively, whereas average mean temperature was 9.4°C. July was recorded as the warmest month. During daytime, wind speed was four times higher than that at night-time. The average daytime and …

Graphical Models Of Residue Coupling In Protein Families, John Thomas, Naren Ramakrishnan, Chris Bailey-Kellogg

Computer Science Technical Reports

Identifying residue coupling relationships within a protein family can provide important insights into intrinsic molecular processes, and has significant applications in modeling structure and dynamics, understanding function, and designing new or modified proteins. We present the first algorithm to infer an undirected graphical model representing residue coupling in protein families. Such a model serves as a compact description of the joint amino acid distribution, and can be used for predictive (will this newly designed protein be folded and functional?), diagnostic (why is this protein not stable or functional?), and abductive reasoning (what if I attempt to graft features of one …

Simulations Of Proton Order And Disorder In Ice Ih, Steven W. Rick

Chemistry Faculty Publications

Computer simulations of ice Ih with different proton orientations are presented. Simulations of proton disordered ice are carried out using a Monte Carlo method which samples over proton degree of freedom, allowing for the calculation of the dielectric constant and for the examination of the degree of proton disorder. Simulations are also presented for two proton ordered structures of ice Ih, the ferroelectric Cmc2₁ structure or ice XI and the antiferroelectric Pna2₁ structure. These simulations indicate that a transition to a proton ordered phase occurs at low temperatures (below 80 K). The …

Magnetic Characterization Of Nanocrystalline Nickel Ferrite Films Processed By A Spin-Spraying Method, Gabriel Caruntu, I Dumitru, Bush Gg, Daniela Caruntu, Charles J. O'Connor

Chemistry Faculty Publications

Highly crystalline nickel ferrite films with different chemical compositions were processed via the spin-spraying method and their morphological, structural and magnetic properties were subsequently investigated. Regardless of the chemical composition, films with variable thicknesses are constructed by 200–400 nm spherical grains grown in the direction normal to the substrate surface. Magnetization measurements show that the spinel ferrite films present a hysteretic behaviour at room temperature with a randomly oriented in-plane easy axis and an anisotropy constant K1 ≈ −2.5 × 104 erg cm−3 Furthermore, the absence of an angular dependence of the coercivity for the in-plane measurement of …

Chromospherically Active Stars. Xxiv. The Giant, Single-Lined Binaries Hd 37824, Hd 181809, And Hd 217188, Francis C. Fekel, Gregory W. Henry

Information Systems and Engineering Management Research Publications

We have obtained spectroscopy and photometry of three chromospherically active, single-lined spectroscopic binaries, HD 37824 (V1149 Ori), HD 181809 (V4138 Sgr), and HD 217188 (AZ Psc). HD 37824 has a circular orbit with a period of 53.57 days. Its primary is a K0 III star, while the secondary is likely a G or K dwarf. HD 181809 has an orbit with a period of 13.04667 days and a low eccentricity of 0.040. The primary has a spectral type of K0 III-IV, and its secondary is probably an M dwarf. The orbit of HD 217188 has a period of 47.1209 days …

Panoramic Images For Situational Awareness In A 3d Chart-Of-The-Future Display, Matthew D. Plumlee, Colin Ware, Roland J. Arsenault, Rick Brennan

Center for Coastal and Ocean Mapping

Many early charts featured sketches of the coastline, providing a good picture of what the shore looked like from the bridge of a ship. These helped the mariner to distinguish one port from another during an approach and establish their rough position within that approach. More recent experimental 3D chart interfaces have incorporated 3D models of land topography and man-made structures to perform the same function. However, topography is typically captured from the air, by means of stereophotogrammetry or lidar and fails to present a good representation of what is seen from a vessel’s bridge. We have been conducting an …

Multi-Dimensional, Multi-National, Multi-Faceted Hydrographic Training: The Nippon Foundation Gebco Training Program At The University Of New Hampshire, Dave Monahan, Clive Angwenyi, Hugo Montoro, Taisei Morishita, Abubakar Mustapha, Walter Reynoso Peralta, Shereen Sharma, Srinivas Karlapati

Center for Coastal and Ocean Mapping

Hydrographic training entered a new era when students arrived at the University of New Hampshire in August of 2004 to form the first class of the Nippon Foundation GEBCO (General Bathymetric Chart of the Oceans) training program. Born out of the need to replenish GEBCO’s aging human material, and of the desire to spread deep ocean mapping capabilities more widely throughout the world, the program attracted applications from 57 students in over thirty countries. The seven selected each had post graduate training and several years experience, but differed in that three were hydrographers, two geologists and two oceanographers. Classes planned …

Microchipping People: The Rise Of The Electrophorus, Katina Michael, M G. Michael

Faculty of Informatics - Papers (Archive)

Automatic identification (auto-ID) is the process of identifying a living or nonliving thing without direct human intervention. Before auto-ID only manual identification techniques existed, such as tattoos and fingerprints, which did not allow for the automatic capture of data. Many researchers credit the vision of a cashless society to the capabilities of auto-ID. Since the 1960s automatic identification has proliferated especially for mass-market applications such as electronic banking and citizen ID. Together with increases in computer processing power, storage equipment and networking capabilities, miniaturization and mobility have heightened the significance of auto-ID to e-business, especially mobile commerce. Citizens are now …

Efficient Join Processing Over Uncertain Data Technical Report, Reynold Cheng, Yuni Xia, Sunil Prabhakar, Rahul Shah, Jeffrey S. Vitter

Department of Computer Science Technical Reports

No abstract provided.

High Throughput Routing In Hybrid Cellular And Ad Hoc Networks, Ioannis Ioannidis, Bogdan Carbunar, Cristina Nita-Rotaru

Department of Computer Science Technical Reports

No abstract provided.