Social and Behavioral Sciences Commons^™

Beta-2 Glycoprotein1: Function In Health And Disease, Spyridon Miyakis, Bill Giannakopoulos, S A. Krilis

Faculty of Science, Medicine and Health - Papers: part A

Beta-2 glycoprotein I (β2GPI) is the principal target of autoantibodies in the antiphospholipid syndrome (APS). It is abundant in human plasma and shares high homology between different mammalian species. Although the exact physiological function of β2GPI has not been fully elucidated, several interactions have been described with other proteins and with negatively charged surfaces, such as anionic phospholipids, dextran and heparin. β2GPI is involved in the coagulation pathway, exerting both procoagulant and anticoagulant activities. Plasma from β2GPI-deficient mice exhibits impaired thrombin generation in vitro. Recently, it has been demonstrated that β2GPI binds factor (F) XI in vitro at concentrations lower …

Oxaliplatin, Fluorouracil, And Leucovorin As Adjuvant Treatment For Colon Cancer, Thierry Andre, Corrado Boni, Lamia Mounedji-Boudiaf, Matilde Navarro, Josep Tabernero, Tamas Hickish, Clare Topham, Marta Zaninelli, Phillip Clingan, John Bridgewater, Isabelle Tabah-Fisch, Aimery De Gramont

Faculty of Science, Medicine and Health - Papers: part A

BACKGROUND: The standard adjuvant treatment of colon cancer is fluorouracil plus leucovorin (FL). Oxaliplatin improves the efficacy of this combination in patients with metastatic colorectal cancer. We evaluated the efficacy of treatment with FL plus oxaliplatin in the postoperative adjuvant setting. METHODS: We randomly assigned 2246 patients who had undergone curative resection for stage II or III colon cancer to receive FL alone or with oxatiplatin for six months. The primary end point was disease-free survival. RESULTS: A total of 1123 patients were randomly assigned to each group. After a median follow-up of 37.9 months, 237 patients in the group …

Editorial Essay, Jennifer Cypher, Catherine Phillips

Faculty of Science, Medicine and Health - Papers: part A

As we put this issue together the last days of 2003 swirled around us, with all the artifice and artifacts that this time of year seems to generate. The steamy-windowed café where we planned the final week of tasks was full of holiday greenery, all artificial. Part of our conversation revolved around the artifacts still to be purchased or made for friends and family. The reality of our intimate connections with artifacts and artifice seems heightened at this time of year. We buy and bake and celebrate, undeniably caught up in webs of things and crafts and greenery (artificial or …

Determining Rotational Temperatures From The Oh(8-3) Band, And A Comparison With (Oh96-2) Rotational Temperatures At Davis, Antarctica, Frances Phillips, G B. Burns, W J R French, P F B Williams, A R. Klekociuk, R P. Lowe

Faculty of Science, Medicine and Health - Papers: part A

Rotational temperatures derived from the OH(8–3) band may vary by ~18K depending on the choice of transition probabilities. This is of concern when absolute temperatures or trends determined in combination with measurements of other hydroxyl bands are important. In this paper, measurements of the OH(8–3) temperature-insensitive Q/P and R/P line intensity ratios are used to select the most appropriate transition probabilities for use with this band. Aurora, airglow and solar and telluric absorption in the OH(8–3) band are also investigated. Water vapour absorption of P₁(4), airglow or auroral contamination of P₁(2) and solar absorption in the …

Geomagnetic Activity Forcing Of The Northern Annular Mode Via The Stratosphere, Daniel Palamara, Edward Bryant

Faculty of Science, Medicine and Health - Papers: part A

We consider various aspects of the link between solar-modulated geomagnetic activity and the Northern Annular Mode (NAM). Our results indicate that the geomagnetic forcing of atmospheric circulation in the Northern Hemisphere is temporally and seasonally restricted, modulated by the Quasi-Biennial Oscillation (QBO), and reliant on stratosphere-troposphere coupling. When the data are restricted to January values after 1965, for years in which the January QBO is eastwards, the correlation coefficient between the geomagnetic AA index and the NAM is 0.85. These results can account for many of the enigmatic features of Northern Hemisphere circulation.

Serotypes And Virulence Gene Profiles Of Shiga Toxin-Producing Escherichia Coli Strains Isolated From Feces Of Pasture-Fed And Lot-Fed Sheep, Steven Djordjevic, Vidiya Ramachandran, Karl A Bettelheim, Barbara A. Vanselow, Peter Holst, Graham Bailey, Michael A. Hornitsky

Faculty of Science, Medicine and Health - Papers: part A

Shiga toxin-producing Escherichia coli (STEC) strains possessing genes for enterohemolysin (ehxA) and/or intimin (eae), referred to here as complex STEC (cSTEC), are more commonly recovered from the feces of humans with hemolytic uremic syndrome and hemorrhagic colitis than STEC strains that do not possess these accessory virulence genes. Ruminants, particularly cattle and sheep, are recognized reservoirs of STEC populations that may contaminate foods destined for human consumption. We isolated cSTEC strains from the feces of longitudinally sampled pasture-fed sheep, lot-fed sheep maintained on diets comprising various combinations of silage and grain, and sheep simultaneously grazing pastures …

Fish Recolonization In Temperate Australian Rockpools: A Quantitative Experimental Approach, Shane Griffiths, Ronald West, Andrew R. Davis, Ken Russell

Faculty of Science, Medicine and Health - Papers: part A

Understanding recolonization processes of intertidal fish assemblages is integral for predicting the consequences of significant natural or anthropogenic impacts on the intertidal zone. Recolonization of experimentally defaunated intertidal rockpools by fishes at Bass Point, New South Wales (NSW), Australia, was assessed quantitatively by using one long-term and two short-term studies. Rockpools of similar size and position at four sites within the intertidal zone were repeatedly defaunated of their fish fauna after one week, one month, and three months during two short-term studies in spring and autumn (5 months each), and every six months for the long-term study (12 months). Fish …

Plant Functional Traits In Relation To Fire In Crown-Fire Ecosystems, Juli G. Pausus, Ross Bradstock, David A. Keith, Jon E. Keeley

Faculty of Science, Medicine and Health - Papers: part A

Disturbance is a dominant factor in many ecosystems, and the disturbance regime is likely to change over the next decades in response to land-use changes and global warming. We assume that predictions of vegetation dynamics can be made on the basis of a set of life-history traits that characterize the response of a species to disturbance. For crown-fire ecosystems, the main plant traits related to postfire persistence are the ability to resprout (persistence of individuals) and the ability to retain a persistent seed bank (persistence of populations). In this context, we asked (1) to what extent do different life-history traits …

Immune Responses Of A Liposome/Iscom Vaccine Adjuvant Against Streptococcal Fibronectin Binding Protein 1 (Sfb1) In Mice, Jason D. Mcarthur, K Schulze, James Chin, B J. Currie, K S. Sriprakash, S R. Talay, G S. Chhatwal, C A. Guzman, Mark J. Walker

Faculty of Science, Medicine and Health - Papers: part A

BACKGROUND & OBJECTIVES: The fibronectin binding protein Sfb1 of Streptococcus pyogenes is a well characterised antigen which induces protection against lethal challenge with group A streptococcus (GAS) when adjuvanted with cholera toxin B-subunit (CTB). As an alternative to CTB adjuvanted intranasal immunisations we investigated the immune responses generated in mice using Sfb1 incorporated in to the skin and mucosal adjuvant SAMA4. METHODS: Mice (BALB/c) were vaccinated intradermally with 100 microl of either SAMA4 (adjuvant only group) or SAMA4/Sfb1 and were boosted 7 days later. Mice vaccinated with CTB based vaccines were immunised by intranasal inoculation with a mixture containing 30 …

Fibronectin-Binding Protein Gene Recombination And Horizontal Transfer Between Group A And G Streptococci, Rebecca J. Towers, Daniel Gal, David Mcmillan, Kadaba S. Sriprakash, Bart J. Currie, Mark J. Walker, Gursharan S. Chhatwal, Peter K. Fagan

Faculty of Science, Medicine and Health - Papers: part A

We report evidence of interspecies gene transfer between the important virulence factor genes sfbI and gfbA. Because the identified group G streptococcus gfbA types possess DNA cassettes that can be identified in a number of group A streptococcus strains, it appears that homologous recombination is occurring between these species.

Effects Of Ultraviolet Radiation And Visible Light On The Development Of Encapsulated Molluscan Embryos, Rachel Przeslawski, Andrew R. Davis, Kirsten Benkendorff

Faculty of Science, Medicine and Health - Papers: part A

Benthic egg masses laid in intertidal habitats are exposed to numerous environmental stresses including potentially damaging ultraviolet radiation (UVR). We sought to determine the developmental effects of UVR and visible light on molluscan embryos within egg masses from habitats with differential UVR exposure. Capsular and gelatinous egg masses from 23 marine gastropod species were collected from 3 intertidal habitats: (1) full sun, (2) partial shade, and (3) full shade. Egg masses were then divided among 4 spectral treatments: full spectrum, no UV-B, no UV, and dark. An ANOVA confirmed that a significant interaction between original habitat and spectral treatment affected …

Where Do Clonal Coral Larvae Go? Adult Genotypic Diversity Conflicts With Reproductive Effort In The Brooding Coral Pocillopora Damicornis, David J. Ayre, Karen Miller

Faculty of Science, Medicine and Health - Papers: part A

Earlier studies of the coral Pocillopora damicornis provide a conflicting picture of its use of sexual and asexual reproduction for population maintenance. In Western Australia, colonies are asexually viviparous, and populations appear to be maintained by localised asexual recruitment but founded by genotypically diverse colonists. However, on Australia¹s Great Barrier Reef (GBR), as in many other regions, populations display little or no evidence of any asexual recruitment. We used allozyme electrophoresis to test for asexual input into local populations of P. damicornis at One Tree Island on the southern GBR. Contrary to expectation we found that all of 136 planulae …

Effects Of Dexamethasone On Posterior Capsule Opacification-Like Changes In A Rat Lens Explant Model, Kylie Mansfield, Anna Cerra, Coral Chamberlain

Faculty of Science, Medicine and Health - Papers: part A

Purpose: Many patients whose sight is initially restored by cataract surgery eventually suffer secondary loss of vision because of posterior capsule opacification (PCO; after-cataract), a condition in which lens epithelial cells left behind at surgery become aberrant and migrate into the light path. The aim of this study was to determine whether dexamethasone (DEX), an anti-inflammatory agent widely used before and after cataract surgery, influences the behavior of lens cells under conditions relevant to PCO development.

Methods: An established rat PCO model was used in which explanted epithelial cells attached to the lens capsule are exposed sequentially to TGFβ2 and …

Fgf-2 Counteracts Loss Of Tgfβ Affected Cells From Rat Lens Explants: Implications For Pco (After Cataract), Kylie Mansfield, Anna Cerra, Coral Chamberlain

Faculty of Science, Medicine and Health - Papers: part A

Purpose: While cataract surgery initially benefits most patients, many suffer secondary loss of vision because of posterior capsule opacification (PCO). Lens epithelial cells left behind at surgery become aberrant and migrate into the light path. TGF-beta (TGFβ) appears to play a key role in this process by inducing the cells to undergo an epithelial-mesenchymal transition. Paradoxically, it also typically induces them to undergo apoptotic death. The present study was undertaken to investigate the hypothesis that FGF plays a role in PCO formation by promoting the survival of abnormal cells with PCO-like characteristics.

Methods: Rat lens epithelial explants were cultured for …

High Prevalence Of Hepatozoon Spp. (Apicomplexa, Hepatozoidae) Infection In Water Pythons (Liasis Fuscus) From Tropical Australia, Beata Ujvari, Thomas Madsen, Mats Olsson

Faculty of Science, Medicine and Health - Papers: part A

Molecular methods were used to identify blood parasites frequently observed in blood smears of water pythons (Liasis fuscus) captured in our study area in the Northern Territory of Australia. A nested polymerase chain reaction (PCR) using primers amplifying the 18s ribosomal RNA (rRNA) nuclear gene resulted in a short PCR product (180 bp) matching this region in the genus Hepatozoon. However, because of the short sequence obtained. 2 new primers were designed based on 18s rRNA sequences of 3 Hepatozoon taxa available in GenBank. Using these primers, approximately 600 bp of the parasite's 18s rRNA gene was amplified successfully and …

Sibudu Cave, Kwazulu-Natal: Background To The Excavations Of Middle Stone Age And Iron Age Occupations, Lyn Wadley, Zenobia Jacobs

Faculty of Science, Medicine and Health - Papers: part A

Sibudu Cave excavations have yielded an Iron Age occupation directly overlying a long sequence of final Middle Stone Age (MSA) layers dating c. 61 000-26 000 years ago. Older, undated layers contain a Howiesons Poort Industry. A diverse animal population is represented in the final MSA. Proxy environmental data suggest cooler, drier conditions with a larger savanna component to the vegetation than at present.

Gay And Lesbian Public History In Australia, Andrew Gorman-Murray

Faculty of Science, Medicine and Health - Papers: part A

Although scholarly interest in gay and lesbian history broadly defined is relatively young, research has increased significantly since the early 1990s. This was largely stimulated by the gay liberation movements of the 1970s and 1980s which both encouraged collective self-awareness amongst gays and sought acceptance from the broader straight society. During the 1990s gays became a significant 'consumer' group targeted by various companies and non-commercial organisations as a niche market. One need only scan through gay community periodicals such as SX or Sydney Star Observer to appreciate the number of businesses and non-commercial groups servicing the gay community. ln light …

Estimated Intake Of Milk Fat Is Negatively Associated With Cardiovascular Risk Factors And Does Not Increase The Risk Of A First Acute Myocardial Infarction. A Prospective Case-Control Study, Eva Warensjo, Jan-Hakan Jansson, Lars Berglund, Kurt Boman, Bo Ahren, Lars Weinehall, Bernt Lindahl, Goran Hallmans, Bengt Vessby

Faculty of Science, Medicine and Health - Papers: part A

Milk fat is high in saturated fatty acids (SFA) and high intakes of SFA are associated with cardiovascular diseases. The aim of the present study was to prospectively evaluate the potential risk of a first-ever acute myocardial infarction (AMI) in relation to the estimated milk-fat intake, reflected as the proportions of pentadecanoic acid (15:0) and heptadecanoic acid (17:0) in serum lipid esters. This was evaluated in a study population selected within the Västerbotten Intervention Program and the northern Sweden ‘Monitoring of Trends and Determinants in Cardiovascular disease’ survey populations. A prospective case–control design was used. The proportions of the biomarkers …

Minimum Energy As The General Form Of Critical Flow And Maximum Flow Efficiency And For Explaining Variations In River Channel Pattern, He Qing Huang, Howard H. Chang, Gerald Nanson

Faculty of Science, Medicine and Health - Papers: part A

Although the Bélanger-Böss theorem of critical flow has been widely applied in open channel hydraulics, it was derived from the laws governing ideal frictionless flow. This study explores a more general expression of this theorem and examines its applicability to flow with friction and sediment transport. It demonstrates that the theorem can be more generally presented as the principle of minimum energy (PME), with maximum efficiency of energy use and minimum friction or minimum energy dissipation as its equivalents. Critical flow depth under frictionless conditions, the best hydraulic section where friction is introduced, and the most efficient alluvial channel geometry …

Mapping Tropical Cyclone Disturbance Of The Great Barrier Reef, Marji Puotinen

Faculty of Science, Medicine and Health - Papers: part A

Tropical cyclones periodically cross the Great Barrier Reef (GBR). The large waves they generate can damage coral reefs. This paper will describe the challenges of mapping cyclone disturbance across such a vast geographic region, and how cartographic visualisation can help us understand the implications of these challenges on the quality of the results.

Compartmentalization Of Prion Isoforms Within The Reproductive Tract Of The Ram, Heath W. Ecroyd, Pierre Sarradin, Jean-Louis Dacheux, Jean-Luc Gatti

Faculty of Science - Papers (Archive)

Cellular prion protein (Prp(C)) is a glycoprotein usually associated with membranes via its glycosylphosphatidylinositol (GPI) anchor. The trans-conformational form of this protein (Prp(SC)) is the suggested agent responsible for transmissible neurodegenerative spongiform encephalopathies. This protein has been shown on sperm and in the reproductive fluids of males. Antibodies directed against the C-terminal sequence near the GPI-anchor site, an N-terminal sequence, and against the whole protein showed that the Prp isoforms were compartmentalized within the reproductive tract of the ram. Immunoblotting with the three antibodies showed that the complete protein and both N- and C-terminally truncated and glycosylated isoforms are present …

The Development Of Signal Transduction Pathways During Epididymal Maturation Is Calcium Dependent, Heath W. Ecroyd, Kelly Asquith, Russell C. Jones, Robert J. Aitken

Faculty of Science - Papers (Archive)

Capacitation has been correlated with the activation of a cAMP-PKA-dependent signaling pathway leading to protein tyrosine phosphorylation. The ability to exhibit this response to cAMP matures during epididymal maturation in concert with the ability of the sperrnatozoa to capacitate. In this study, we have addressed the mechanisms by which spermatozoa gain the potential to activate this signaling pathway during epididymal maturation. In a modified Tyrode's medium containing 1.7 mM calcium, caput spermatozoa had significantly higher [Ca2+](i) than caudal cells and could not tyrosine phosphorylate in response to cAMP. However, in calcium-depleted medium both caput and caudal cells could exhibit a …

Negotiating Nature: Collaboration And Conflict Between Aboriginal And Conservation Interests In New South Wales, Australia, Michael Adams

Faculty of Science - Papers (Archive)

Faced with the paradox of a large global increase in conservation reserves and a simultaneous global decrease in actual effective protection for biodiversity, conservation scientists and policymakers are questioning established conservation theory and practice. I argue that the fundamental premises, the foundational myths, for Western-style conservation also need to be questioned. The statistics on Indigenous land claims, and conservation reserves, in Australia and more specifically the state of New South Wales (NSW), reveal a landscape of policy failure in both arenas. Focusing on Australia, I use spatial analysis and policy histories to demonstrate converging trajectories of land use priorities for …

Land-Based Activities And Impacts On Coral Reefs And The Marine Environment Of The Pacific Islands, Robert John Morrison, N. S. Tuivavalagi

Faculty of Science - Papers (Archive)

This paper recognizes the fact some of the problems encountered in the coral reefs and marine environment of the Pacific are actually due to land-based activities. This message needs to be clearly brought to the attention of farmers and others who work the land - as well as to fishermen and others whose activities focus on coral reefs and the marine environment.

The paper discusses the Pacific environment and some relevant key issues. The paper goes on to discuss land-based activities that harm the coral reef environment - with some emphasis on agricultural activities and soil erosion; however, the impacts …

Charge-On-Spring Polarizable Water Models Revisited: From Water Clusters To Liquid Water To Ice, Haibo Yu, Wilfred Van Gunsteren

Faculty of Science - Papers (Archive)

The properties of two improved versions of charge-on-spring �COS� polarizable water models (COS/G2 and COS/G3) that explicitly include nonadditive polarization effects are reported. In COS models, the polarization is represented via a self-consistently induced dipole moment consisting of a pair of separated charges. A previous polarizable water model (COS/B2), upon which the improved versions are based, was developed by Yu, Hansson, and van Gunsteren �J. Chem. Phys. 118, 221 �2003��. To improve the COS/B2 model, which overestimated the dielectric permittivity, one additional virtual atomic site was used to reproduce the water monomer quadrupole moments besides the water monomer dipole moment …

Molecular Dynamics Simulations Of Peptides Containing An Unnatural Amino Acid: Dimerization, Folding, And Protein Binding, Haibo Yu, Xavier Daura, Wilfred Van Gunsteren

Faculty of Science - Papers (Archive)

We have performed molecular dynamics (MD) simulations to study the dimerization, folding, and binding to a protein of peptides containing an unnatural amino acid. NMR studies have shown that the substitution of one residue in a tripeptide β-strand by the unnatural amino acid Hao (5-HO2CCONH-2-MeO-C6H3-CO-NHNH2) modifies the conformational flexibility of the β-strand and the hydrogen-bonding properties of its two edges: The number of hydrogen-bond donors and acceptors increases at one edge, whereas at the other, they are sterically hindered. In simulations in chloroform, the Hao-containing peptide 9 (i-PrCO-Phe-Hao-Val-NHBu) forms a β-sheet–like hydrogen-bonded dimer, in good agreement with the available experimental …

Western Pacific Coral Delta18o Records Of Anomalous Holocene Variability In El Nino-Southern Oscillation, Helen V. Mcgregor, M Gagan

Faculty of Science - Papers (Archive)

No abstract provided.

Processes Controlling Metal Transport And Retention As Metal-Contaminated Groundwaters Efflux Through Estuarine Sediments, Stuart L. Simpson, Edward J. Maher, Dianne F. Jolley

Faculty of Science - Papers (Archive)

Factors affecting the transport and retention of Cd, Cr, Cu, Ni, Pb and Zn in acidic groundwaters as they pass through estuarine sediments were investigated using column experiments. Acidic groundwaters caused the rapid dissolution of iron sulfide (AVS) and other iron and manganese phases from sediments that are important for metal binding and buffering. Metal breakthrough to overlying water occurred in the order of Ni>Zn>Cd>>Cu>>Cr/Pb. Metal transport increased as the sediment permeability increased, reflecting the low resistance to flow caused by larger sand-sized particles and the decreased abundance of metal adsorption sites on these materials. Metal …

Selenium Accumulation In The Cockle Anadara Trapezia, Dianne F. Jolley, William A. Maher, Jennelle Kyd

Faculty of Science - Papers (Archive)

An extensive study on Se accumulation in a population of Anadara trapezia from a marine lake is reported. The effects of organism mass, gender, reproductive cycle, and season on Se accumulation and tissue distribution were investigated. Analyses showed that gender and reproductive cycle had no significant effect on Se accumulation. A. trapezia showed a strong positive correlation between Se burden and tissue mass. Constant Se concentrations were observed within individual populations but varied spatially with sediment Se concentrations. Se concentrations in tissues decreased from gills>gonad/intestineOmantleO muscleOfoot, which remained constant over 12 months, however, significantly lower concentrations were observed in …

Photosynthesis In Silico. Overcoming The Challenges Of Photosynthesis Education Using A Multimedia Cd-Rom, A. W. Russell, G. M.A. Netherwood, Sharon A. Robinson

Faculty of Science - Papers (Archive)

Photosynthesis is a central topic in biology education. It remains one of the most challenging, largely because of a) its conceptual difficulty, leading to lack of interest and misconceptions among students; b) the difficulties students have in visualising the process, or relating it to things they can see, especially when the topic is presented purely as a molecular process; and c) limitations to the practical demonstration of photosynthesis because equipment is either cheap, unreliable and antiquated or prohibitively expensive. In response, we have combined expertise in photosynthesis research and education, and in graphic design, to produce an interactive, multimedia package, …