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Articles 15181 - 15210 of 302419

Full-Text Articles in Physical Sciences and Mathematics

R Text Analysis For Adam Smith Cie Selected Works, Charlotte Grahame Apr 2023

R Text Analysis For Adam Smith Cie Selected Works, Charlotte Grahame

Mathematics and Computer Science Presentations

Text mining and text analysis is a way of understanding text documents using r coding that is more frequently used for numbered data. It helps with understanding portions of the text and drawing conclusions from there. This research looks specifically at the Adam Smith required documents that are used in the CIE course designated for freshmen. It looks at sentiments of the documents, including word sentiment, sentence sentiment, page and overall document sentiment as well. It provides visuals of word clouds to portray word frequency, tf-idf (which is explained in the presentation) and bigram analysis.


Cie Text Analysis: Narrative Of The Life Of Frederick Douglass, The Declaration Of Independence, And The Declaration Of Sentiments, Arianna Knipe Apr 2023

Cie Text Analysis: Narrative Of The Life Of Frederick Douglass, The Declaration Of Independence, And The Declaration Of Sentiments, Arianna Knipe

Mathematics and Computer Science Presentations

Our STAT-451 class has worked with analyzing the words from CIE texts and assigning them to a sentiment or feeling and comparing them with one another using RStudio. This project analyzes texts from three sources: The Narrative of the Life of Frederick Douglass, The Declaration of Independence and the Declaration of Sentiments.


Applying Bioactive Glass Beads For Long-Term Drug Delivery, Marie O. Sykes, Rashi Sharma Apr 2023

Applying Bioactive Glass Beads For Long-Term Drug Delivery, Marie O. Sykes, Rashi Sharma

Physics and Astronomy Presentations

Bioactive glass beads have been used to distribute medicine over extended periods of time in limited uses. They can be engineered to have a porous and hollow structure, and using Mo-Sci's OL-GL 1756b (1756b), the fundamental notion of dosage distribution can be tested in preparation to design the optimal glass for medicine distribution. A few key factors in this is how long does it take the liquid to release from the beads, how do the beads decay, and how well the glass can bond to human tissue. This research demonstrates the capacities of the 1756b type as a promising start …


Translation Of: Sur Des Familles D’Hypersurfaces Isoparamétriques Des Espaces Sphériques À 5 Et À 9 Dimensions, Revista Univ. Tucuman, Serie A, 1 (1940), 5–22, By Elie Cartan, Thomas E. Cecil Apr 2023

Translation Of: Sur Des Familles D’Hypersurfaces Isoparamétriques Des Espaces Sphériques À 5 Et À 9 Dimensions, Revista Univ. Tucuman, Serie A, 1 (1940), 5–22, By Elie Cartan, Thomas E. Cecil

Mathematics and Computer Science Department Faculty Scholarship

English title: On Families of Isoparametric Hypersurfaces in Spherical Spaces of 5 and 9 Dimensions

This is an English translation of the article "Sur des familles d'hypersurfaces isoparamétriques des espaces sphériques à 5 et à 9 dimensions" which was originally published in Revista Univ. Tucuman, Serie A, 1, pp. 5-22 (1940), by Élie Cartan.

A note from Thomas E. Cecil, translator: This is an unofficial translation of the original paper which was written in French. All references should be made to the original paper.

Mathematics Subject Classification Numbers: 53B25, 53C40, 53C42


Counterventions: A Reparative Reflection On Interventionist Hci, Rua Mae Williams, Louanne E. Boyd, Juan E. Gilbert Apr 2023

Counterventions: A Reparative Reflection On Interventionist Hci, Rua Mae Williams, Louanne E. Boyd, Juan E. Gilbert

Engineering Faculty Articles and Research

Research in HCI applied to clinical interventions relies on normative assumptions about which bodies and minds are healthy, valuable, and desirable. To disrupt this normalizing drive in HCI, we define a “counterventional approach” to intervention technology design informed by critical scholarship and community perspectives. This approach is meant to unsettle normative assumptions of intervention as urgent, necessary, and curative. We begin with a historical overview of intervention in HCI and its critics. Then, through reparative readings of past HCI projects in autism intervention, we illustrate the emergent principles of a counterventional approach and how it may manifest research outcomes that …


Cooperative Heterometallic Platforms Enabling Selective C–H Bond Activation And Functionalization Of Pyridines, Manar M. Shoshani Apr 2023

Cooperative Heterometallic Platforms Enabling Selective C–H Bond Activation And Functionalization Of Pyridines, Manar M. Shoshani

Chemistry Faculty Publications and Presentations

The development of methodologies for regioselective C–H functionalization of N-heterocycles has seen sustained growth over the last two decades. Cooperative reactivity stemming from the addition of Lewis-acidic metals to late-transition-metal complexes has enabled regioselective catalytic transformations. Progress in the design of ligands and Lewis-acidic metal precursors has led to development of in situ cooperative catalysts able to facilitate C–H functionalization of pyridine at C-2, C-3, and C-4 positions. Very recently, focused efforts toward well-defined heterometallic complexes facilitating C-H bond catalysis have been targeted. Transformations showcasing regioselective C-2 transformations have been achieved. This perspective examines select examples delineating the evolution …


Building Credibility, Trust, And Safety On Video-Sharing Platforms, Shuo Niu, Zhicong Lu, Amy X. Zhang, Jie Cai, Carla F. Griggio, Hendrick Heuer Apr 2023

Building Credibility, Trust, And Safety On Video-Sharing Platforms, Shuo Niu, Zhicong Lu, Amy X. Zhang, Jie Cai, Carla F. Griggio, Hendrick Heuer

Computer Science

Video-sharing platforms (VSPs) such as YouTube, TikTok, and Twitch attract millions of users and have become influential information sources, especially among the young generation. Video creators and live streamers make videos to engage viewers and form online communities. VSP celebrities obtain monetary benefits through monetization programs and affiliated markets. However, there is a growing concern that user-generated videos are becoming a vehicle for spreading misinformation and controversial content. Creators may make inappropriate content for attention and financial benefits. Some other creators also face harassment and attack. This workshop seeks to bring together a group of HCI scholars to brainstorm technical …


Loss Scaling And Step Size In Deep Learning Optimizatio, Nora Alosily Apr 2023

Loss Scaling And Step Size In Deep Learning Optimizatio, Nora Alosily

Dissertations

Deep learning training consumes ever-increasing time and resources, and that is
due to the complexity of the model, the number of updates taken to reach good
results, and both the amount and dimensionality of the data. In this dissertation,
we will focus on making the process of training more efficient by focusing on the
step size to reduce the number of computations for parameters in each update.
We achieved our objective in two new ways: we use loss scaling as a proxy for
the learning rate, and we use learnable layer-wise optimizers. Although our work
is perhaps not the first …


Ecomves: Enhancing Comves Using Data Piggybacking For Resource Discovery At The Network Edge, Sanzida Hoque Apr 2023

Ecomves: Enhancing Comves Using Data Piggybacking For Resource Discovery At The Network Edge, Sanzida Hoque

Theses

Over the past few years, Augmented Reality (AR) and Virtual Reality (VR) have emerged as highly popular technologies that demand rapid and efficient processing of data with low latency and high bandwidth, in order to enable seamless real-time interaction between users and the virtual environment. This presents challenges for network infrastructure design, which can be addressed through edge computing. However, edge computing also presents challenges, such as selecting the appropriate edge server for computing tasks in dynamic networks with rapidly changing resource availability. Named Data Networking (NDN) is a potential future Internet architecture that could provide a balanced distribution of …


A Large-Scale Survey Of Brown Dwarf Atmospheres, Savanah Kay Turner Apr 2023

A Large-Scale Survey Of Brown Dwarf Atmospheres, Savanah Kay Turner

Theses and Dissertations

Brown dwarfs are substellar objects that fall in-between the smallest stars and largest planets in size and temperature. Due to their relatively cool temperatures, the atmospheres of these 'failed stars' have been shown to exhibit interesting properties such as iron, silicate, and salt clouds. Theoretical atmospheric models based on known physics and chemistry can be used as tools to interpret and understand our observations of brown dwarfs. I have fit archival and new infrared spectra of over 300 brown dwarfs with atmospheric models. Using the parameters of the best-fit models as estimates for the physical properties of the brown dwarfs …


Evaluation Of Genetic Diversity In White Clover (Trifolium Repens L.) Through Measurement Of Simple Sequence Repeat (Ssr) Polymorphism, J. George, E. Van Zijll De Jong, T. C. Wilkinson, M. P. Dobrowolski, N. O. I. Cogan, K. F. Smith, J. W. Forster Apr 2023

Evaluation Of Genetic Diversity In White Clover (Trifolium Repens L.) Through Measurement Of Simple Sequence Repeat (Ssr) Polymorphism, J. George, E. Van Zijll De Jong, T. C. Wilkinson, M. P. Dobrowolski, N. O. I. Cogan, K. F. Smith, J. W. Forster

IGC Proceedings (1993-2023)

White clover (Trifolium repens L.) is a key important temperate pasture legume. Due to the obligate outbreeding nature of white clover, individual genotypes within cultivars are highly genetically heterogeneous. Genetic diversity has been assessed within and between 16 elite cultivars derived from Europe, North and South America, New Zealand and Australia.


Genetic Diversity Among Alfalfa Cultivars Using Ssr Markers, S. Flajoulot, J. Ronfort, P. Baudouin, T. Huguet, P. Barre, Christian Huyghe, B. Julier Apr 2023

Genetic Diversity Among Alfalfa Cultivars Using Ssr Markers, S. Flajoulot, J. Ronfort, P. Baudouin, T. Huguet, P. Barre, Christian Huyghe, B. Julier

IGC Proceedings (1993-2023)

Alfalfa (Medicago sativa) is an autotetraploid, allogamous and heterozygous species. Cultivated varieties are synthetic cultivars, usually obtained through 3 or 4 generations of panmictic reproduction of a set of various numbers of parents. The parents can be clones, half-sib or full-sib families. The breeders apply selection pressure for some agronomic traits, to induce changes in the genetic background. The objective of this study was to investigate the differentiation level among seven cultivars originating from one breeding program, and between these cultivars and the breeding pool, with eight SSR markers.


Random Amplified Polymorphic Dna Analysis In Section Pnigma Of The Genus Bromus L., M. Tuna, O. Barzani, K. P. Vogel, Avi Golan-Goldhirsh Apr 2023

Random Amplified Polymorphic Dna Analysis In Section Pnigma Of The Genus Bromus L., M. Tuna, O. Barzani, K. P. Vogel, Avi Golan-Goldhirsh

IGC Proceedings (1993-2023)

The section Pnigma consists of about 60 species. It was shown that Eurasian species of the section with the same ploidy level have similar nuclear DNA contents (Tuna et al., 2001). A linear correlation between nuclear DNA content and ploidy level was also found (Tuna et al., 2001). Furthermore, karyotypes of Eurasian species are similar (Tuna et al., 2004a) but differ from the North American species (Tuna et al., 2004b). Yet, the genetic relationship among species within the section is poorly known (Armstrong, 1991). The objective of this study was to assess the phylogenetic relationships among …


Utilization Of Ssr To Distinguish Alfalfa Cultivars, G. R. Bauchan, C. He, Z-L. Xia Apr 2023

Utilization Of Ssr To Distinguish Alfalfa Cultivars, G. R. Bauchan, C. He, Z-L. Xia

IGC Proceedings (1993-2023)

Simple sequence repeat (SSR) or microsatellite markers are co-dominant, abundant and hyper-variable molecular markers from eukaryotic genomes that are being widely used in genetic mapping and phylogenetic studies. Currently, the number of available SSR markers is still very limited for use in alfalfa (Medicago sativa). Thus, this study was conducted to develop SSR from alfalfa genomic libraries and EST and BAC sequence data from M. truncatula for use in distinguishing the nine historically recognized U.S. germplasm sources and eleven fall dormancy check cultivars of alfalfa.


Genetic Diversity In Zoysiagrass Ecotypes Based On Morphological Characteristics And Ssr Markers, M. Hashiguchi, S. Tsuruta, T. Matsuo, M. Ebina, M. Kobayashi, H. Akamine, Ryo Akashi Apr 2023

Genetic Diversity In Zoysiagrass Ecotypes Based On Morphological Characteristics And Ssr Markers, M. Hashiguchi, S. Tsuruta, T. Matsuo, M. Ebina, M. Kobayashi, H. Akamine, Ryo Akashi

IGC Proceedings (1993-2023)

Zoysiagrass consists of a number of interfertile species, some of which are important grasses for turfgrass and grazing pasture in Japan. Recently, we developed simple sequence repeats (SSRs) markers from Zoysia japonica “Asagake” genomic DNA by enriched genomic library method (Yamamoto et al., 2002). Here we identify genetic diversity in 38 ecotypes of zoysiagrass (Z. matrella and Z. tenuifolia) from a group of southwest islands of Japan based on morphological characteristics and SSR markers.


Genetic Diversity In Colonial Bentgrass (Agrostis Capillaris L.) Revealed By Ecori/Msei And Psti/Msei Aflp Markers, H. Zhao, S. Bughrara Apr 2023

Genetic Diversity In Colonial Bentgrass (Agrostis Capillaris L.) Revealed By Ecori/Msei And Psti/Msei Aflp Markers, H. Zhao, S. Bughrara

IGC Proceedings (1993-2023)

Colonial bentgrass species (Agrostis capillaris L.) is a potential genetic resource for the improvement of other bentgrass species (Agrostis spp.) with regard to resistance to environmental stresses and diseases. Transferring resistance from colonial to other bentgrass species is a promising goal in turfgrass breeding programs (Belanger, 2003). Assessment of genetic diversity among accessions of colonial bentgrass species will contribute to eliminate undesirable duplications in the germplasm collection and increase the efficiency of research efforts. It will allow researcher to select diverse resistance genes from different sources to incorporate and pyramid these resistance genes into creeping or other bentgrass …


Databases For Managing Genetic Resources Collections And Mapping Populations Of Forage And Related Species, I. Thomas, H. J. Ougham, D. Peltier Apr 2023

Databases For Managing Genetic Resources Collections And Mapping Populations Of Forage And Related Species, I. Thomas, H. J. Ougham, D. Peltier

IGC Proceedings (1993-2023)

Effective management of plant material used in crop improvement and underpinning research is greatly facilitated by a properly designed data structure accessible by all those working with the material. At IGER we have developed the Aberystwyth Genetic Resources Information System, AGRIS, for managing genetic resources acquired through collecting trips, seed exchange, breeding and transgenic programmes. Recently this has been complemented by MaPIS, a Mapping Populations Information System, which links with AGRIS and allows for storage and documentation of information about plant mapping populations, including pedigrees, status and physical locations of accessions and individual genotypes. IGER also maintains the European Central …


The Importance Of Exotic Forage Germplasm In Feeding New Zealand's Livestock, J. Lancashire Apr 2023

The Importance Of Exotic Forage Germplasm In Feeding New Zealand's Livestock, J. Lancashire

IGC Proceedings (1993-2023)

A number of recent reports in New Zealand have expressed the view that restricted access to new plant genetic material from overseas is a major risk to the future growth of the primary sector (MAF, 2002; Douglas, 2003). The restrictions outlined in this paper are the result of the Hazardous Substances and New Organisms (HSNO) Act and regulations administered by MAF and the Environmental Risk Management Authority (ERMA) since July 1998. This paper reviews the historical role of exotic forage germplasm in plant improvement in New Zealand, and quantifies the current contribution of recently imported plant material to exports from …


Growth, Nitrogen And Phosphorus Economy In Two Lotus Glaber Mill. Cytotypes Grown Under Contrasting P-Availability, D. H. Cogliatti, L. A. Lett, M. S. Barufaldi, P. Segura, J. A. Cardozo Apr 2023

Growth, Nitrogen And Phosphorus Economy In Two Lotus Glaber Mill. Cytotypes Grown Under Contrasting P-Availability, D. H. Cogliatti, L. A. Lett, M. S. Barufaldi, P. Segura, J. A. Cardozo

IGC Proceedings (1993-2023)

Lotus glaber Mill. (lotus) is a forage legume with its origin in Europe which has shown an excellent adaptation to the Depressed Pampas of the Province of Buenos Aires, Argentina. The soils colonized by lotus usually have poor drainage, moderate sodium and low extractable P concentrations. An experiment was performed with the aim of comparing the early growth and economy of phosphorus (P) and nitrogen (N) within two L. glaber cytotypes differing in their ploidy level, a commercial diploid versus an induced autotetraploid population (Barufaldi et al., 2001).


Application Of Molecular Diversity In A Forage Grass Breeding Program, Andrew A. Hopkins, M. C. Saha Apr 2023

Application Of Molecular Diversity In A Forage Grass Breeding Program, Andrew A. Hopkins, M. C. Saha

IGC Proceedings (1993-2023)

Little or no genotypic information is available for many forage grass populations. The degree of genetic similarity within and among populations greatly influences the choice of breeding strategies and germplasm for developing improved cultivars. Molecular markers have proven effective in classifying genetic diversity of a number of perennial grasses (e.g. Fu et al., 2004; Kubik et al., 2001). We present here an overview of our efforts to integrate molecular diversity data into our breeding program.


Use Of Cellular Automata Modelling Approaches To Understand Potential Impacts Of Gm Grasses On Grassland Communities, R. Colasanti, R. Hunt, L. S. Watrud Apr 2023

Use Of Cellular Automata Modelling Approaches To Understand Potential Impacts Of Gm Grasses On Grassland Communities, R. Colasanti, R. Hunt, L. S. Watrud

IGC Proceedings (1993-2023)

In order to predict the potential unintended ecological impacts of genetically modified (GM) grasses, we must understand how the engineered traits, in this case herbicide resistance, are expressed in an ecological context. It would be a daunting task to experimentally evaluate the full multiplicity of potential pair-wise interactions between GM plants and native plants under a broad variety of actual environmental conditions. We have employed the modelling methodology of cellular automata (CA), where a plant's distribution within a two-dimensional environmental grid is determined by rules relating to phenomena such as seed dispersal, clonal expansion and interactions with adjacent plants. We …


Shutting The Stable Door After The Horse Has Bolted? Risk Assessment And Regulation For Transgenic Forages, Chris J. Pollock Apr 2023

Shutting The Stable Door After The Horse Has Bolted? Risk Assessment And Regulation For Transgenic Forages, Chris J. Pollock

IGC Proceedings (1993-2023)

The regulation of GM agriculture in Europe is a matter of considerable public interest. The development of a regulatory framework has imposed significant additional costs without generating broad public acceptance. However, the current risk assessment and management framework does provide a basis for considering how the broader issues surrounding the introduction of any novel agricultural technology might be approached.


Development And Field Evaluation Of Transgenic Ryegrass (Lolium Spp.) With Down-Regulation Of Main Pollen Allergens, N. Petrovska, A. Mouradov, Z. Y. Wang, K. F. Smith, G. C. Spangenberg Apr 2023

Development And Field Evaluation Of Transgenic Ryegrass (Lolium Spp.) With Down-Regulation Of Main Pollen Allergens, N. Petrovska, A. Mouradov, Z. Y. Wang, K. F. Smith, G. C. Spangenberg

IGC Proceedings (1993-2023)

Ryegrass (Lolium spp.) pollen is a widespread source of airborne allergens and is a major cause of hayfever and seasonal allergic asthma, which affect approximately 25% of the population in cool temperate climates. The main allergens of ryegrass pollen are the proteins Lol p 1 and Lol p 2. These proteins belong to two major classes of grass pollen allergens to which over 90% of pollen-allergic patients are sensitive. The functional role in planta of these pollen allergen proteins remains largely unknown. The generation, analysis and field evaluation of transgenic plants with reduced levels of the main ryegrass pollen allergens, …


Discovery, Isolation And Characterisation Of Promoters From Perennial Ryegrass (Lolium Perenne), A. Lidgett, N. Petrovska, J. Chalmers, N. Cummings, G. C. Spangenberg Apr 2023

Discovery, Isolation And Characterisation Of Promoters From Perennial Ryegrass (Lolium Perenne), A. Lidgett, N. Petrovska, J. Chalmers, N. Cummings, G. C. Spangenberg

IGC Proceedings (1993-2023)

The availability of a suite of promoters with a range of spatial, temporal and inducible expression patterns is of significant importance to enable targeted expression of genes of interest for molecular breeding of forage species. A range of resources and tools have been developed for promoter isolation and characterisation in perennial ryegrass (Lolium perenne L.) including genomic lambda and BAC libraries and a 15 K unigene microarray.


Improving Forage Quality Of Tall Fescue (Festuca Arundinacea) By Genetic Manipulation Of Lignin Biosynthesis, Z. -Y. Wang, L. Chen, C. -K. Auh, A. Hopkins, P. Dowling Apr 2023

Improving Forage Quality Of Tall Fescue (Festuca Arundinacea) By Genetic Manipulation Of Lignin Biosynthesis, Z. -Y. Wang, L. Chen, C. -K. Auh, A. Hopkins, P. Dowling

IGC Proceedings (1993-2023)

Lignification of plant cell walls is a major factor limiting forage digestibility and concomitantly animal productivity. Improvement in forage grass cell wall digestibility has become an important goal of many plant-ruminant animal research programs. Lignins are complex phenolic heteropolymers associated with the polysaccharidic components of the wall in specific plant cells. Lignin in forage grasses comprises guaiacyl (G) units derived from coniferyl alcohol, syringyl (S) units derived from sinapyl alcohol, and p-hydroxyphenyl (H) units derived from p-coumaryl alcohol. Cinnamyl alcohol dehydrogenase (CAD) and caffeic acid O- methyltransferase (COMT) are key enzymes involved in lignin biosynthesis. Tall fescue …


Manipulating The Phenolic Acid Content And Digestibility Of Forage Grasses By Targeted Expression Of Fungal Cell Wall Degrading Enzymes, M. M. De O. Buanafina, P. Morris, T. Langdon, S. J. Dalton, B. Hauck, H. Porter Apr 2023

Manipulating The Phenolic Acid Content And Digestibility Of Forage Grasses By Targeted Expression Of Fungal Cell Wall Degrading Enzymes, M. M. De O. Buanafina, P. Morris, T. Langdon, S. J. Dalton, B. Hauck, H. Porter

IGC Proceedings (1993-2023)

Grass cell walls constitute 30-80% of forage dry matter, representing a major source of energy for ruminants. Ferulic acid (4-hydroxy-3-methoxy-cinnamic acid) and other hydroxycinnamic acids are ester linked to arabinosyl residues in arabinoxylans of grass cell walls and undergo oxidative coupling reactions resulting in the formation of a variety of dehydrodiferulate dimers which cross-link cell wall polymers. Although such cross-links have a number of important roles in the cell wall, they also hinder the rate and extent of cell wall degradation by ruminant microbial and fungal enzymes. We have shown previously the expression of a ferulic acid esterase gene from …


Agrobacterium Tumefaciens-Mediated Transformation Of Perennial Ryegrass (Lolium Perenne L.), H. Sato, M. Fujimori, Y. Mano, T. Kiyoshi, T. Takamizo Apr 2023

Agrobacterium Tumefaciens-Mediated Transformation Of Perennial Ryegrass (Lolium Perenne L.), H. Sato, M. Fujimori, Y. Mano, T. Kiyoshi, T. Takamizo

IGC Proceedings (1993-2023)

An Agrobacterium tumefaciens-mediated transformation method has several advantages. However, this method has no example of success in perennial ryegrass (Lolium perenne L.). Since Lolium species are outcrossing, one cultivar consists of many genotypes. Each genotype can show a different ability for callus formation and plant regeneration (Takahashi et al., 2004). Thus, it is important to select a good genotype for efficient and stable transformation. If the plant is maintained in vitro, we can perform transformation using calli induced from shoot tips of the same genotype at any time. Our objective is to confirm an A. tumefaciens …


A Comparison Of Hygromycin And Paromomycin Selection Strategies In The Genetic Transformation Of Seven Lolium, Festuca, Poa, And Agrostis Species, S. J. Dalton, P. Robson, M. Buanafina, A. J. E. Bettany, E. Timms, P. Morris Apr 2023

A Comparison Of Hygromycin And Paromomycin Selection Strategies In The Genetic Transformation Of Seven Lolium, Festuca, Poa, And Agrostis Species, S. J. Dalton, P. Robson, M. Buanafina, A. J. E. Bettany, E. Timms, P. Morris

IGC Proceedings (1993-2023)

Hygromycin selection for the hpt gene, expressed from the CaMV-35S promoter, has been successful in transgenesis of a limited number of grass species. As an alternative to hpt selection Altpeter et al., (2000) reported successful transformation using paromomycin selection for the nptII gene expressed by the maize ubiquitin promoter. We have tested the utility of a number of selection cassettes using previously sporadically transformable species which nevertheless had very good tissue culture and regeneration protocols.


Nylon Mesh As An Improved Support For Bombarded Calli Or Cell Suspensions, S. J. Dalton, P. Robson, M. Buanafina, A. J. E. Bettany, E. Timms, D. Wiffen, P. Morris Apr 2023

Nylon Mesh As An Improved Support For Bombarded Calli Or Cell Suspensions, S. J. Dalton, P. Robson, M. Buanafina, A. J. E. Bettany, E. Timms, D. Wiffen, P. Morris

IGC Proceedings (1993-2023)

Using cell suspensions to transform some grass species by particle bombardment has a number of disadvantages including increased somoclonal variation in liquid cell culture and poor performance due to polysaccharide production. The use of calli avoids these problems, but the manipulation of calli through numerous media changes is laborious and time-consuming. We investigated a possible mechanism to facilitate the use of calli in transformation by immobilising calli on mesh.


A Novel Genotype Independent Protocol For In Vitro Plant Regeneration From Mature Seed Derived Callus Of Tall Fescue (Festuca Arundinacea Schreb.), S. Chennareddy, R. V. Sairam, S. L. Goldman Apr 2023

A Novel Genotype Independent Protocol For In Vitro Plant Regeneration From Mature Seed Derived Callus Of Tall Fescue (Festuca Arundinacea Schreb.), S. Chennareddy, R. V. Sairam, S. L. Goldman

IGC Proceedings (1993-2023)

Tall fescues (Festuca arundinacea Schreb.) are cool season forage and turf grasses of significant agricultural importance in different grassland countries. Genetic improvement of tall fescues by conventional selection procedures is slow, since these are predominantly, cross-pollinated, hexaploid and generally infertile (Jauhar, 1993). Genetic Engineering approaches for incorporation of agronomically useful traits may contribute to the development of improved tall fescue cultivars (Spangenberg et al., 1998). However for any genetic engineering studies, it is essential to develop a genotype-independent, reproducible and efficient in vitro plant regeneration protocol. In the present study, we analyzed the effects of different sterilization procedures …